High throughput screening of starch structures using carbohydrate microarrays

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Standard

High throughput screening of starch structures using carbohydrate microarrays. / Tanackovic, Vanja; Rydahl, Maja Gro; Pedersen, Henriette Lodberg; Motawie, Mohammed Saddik; Shaik, Shahnoor Sultana; Mikkelsen, Maria Dalgaard; Jensen, Susanne Langgård; Fangel, Jonatan Ulrik; Willats, William George Tycho; Blennow, Andreas.

I: Scientific Reports, Bind 6, 30551, 2016.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Tanackovic, V, Rydahl, MG, Pedersen, HL, Motawie, MS, Shaik, SS, Mikkelsen, MD, Jensen, SL, Fangel, JU, Willats, WGT & Blennow, A 2016, 'High throughput screening of starch structures using carbohydrate microarrays', Scientific Reports, bind 6, 30551. https://doi.org/10.1038/srep30551

APA

Tanackovic, V., Rydahl, M. G., Pedersen, H. L., Motawie, M. S., Shaik, S. S., Mikkelsen, M. D., Jensen, S. L., Fangel, J. U., Willats, W. G. T., & Blennow, A. (2016). High throughput screening of starch structures using carbohydrate microarrays. Scientific Reports, 6, [30551]. https://doi.org/10.1038/srep30551

Vancouver

Tanackovic V, Rydahl MG, Pedersen HL, Motawie MS, Shaik SS, Mikkelsen MD o.a. High throughput screening of starch structures using carbohydrate microarrays. Scientific Reports. 2016;6. 30551. https://doi.org/10.1038/srep30551

Author

Tanackovic, Vanja ; Rydahl, Maja Gro ; Pedersen, Henriette Lodberg ; Motawie, Mohammed Saddik ; Shaik, Shahnoor Sultana ; Mikkelsen, Maria Dalgaard ; Jensen, Susanne Langgård ; Fangel, Jonatan Ulrik ; Willats, William George Tycho ; Blennow, Andreas. / High throughput screening of starch structures using carbohydrate microarrays. I: Scientific Reports. 2016 ; Bind 6.

Bibtex

@article{539062e621c744de865fe373b97bdc15,
title = "High throughput screening of starch structures using carbohydrate microarrays",
abstract = "In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.",
keywords = "Journal Article",
author = "Vanja Tanackovic and Rydahl, {Maja Gro} and Pedersen, {Henriette Lodberg} and Motawie, {Mohammed Saddik} and Shaik, {Shahnoor Sultana} and Mikkelsen, {Maria Dalgaard} and Jensen, {Susanne Langg{\aa}rd} and Fangel, {Jonatan Ulrik} and Willats, {William George Tycho} and Andreas Blennow",
year = "2016",
doi = "10.1038/srep30551",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - High throughput screening of starch structures using carbohydrate microarrays

AU - Tanackovic, Vanja

AU - Rydahl, Maja Gro

AU - Pedersen, Henriette Lodberg

AU - Motawie, Mohammed Saddik

AU - Shaik, Shahnoor Sultana

AU - Mikkelsen, Maria Dalgaard

AU - Jensen, Susanne Langgård

AU - Fangel, Jonatan Ulrik

AU - Willats, William George Tycho

AU - Blennow, Andreas

PY - 2016

Y1 - 2016

N2 - In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.

AB - In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.

KW - Journal Article

U2 - 10.1038/srep30551

DO - 10.1038/srep30551

M3 - Journal article

C2 - 27468930

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 30551

ER -

ID: 169103211