Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase

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Standard

Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase. / Ignea, Codruta; Trikka, Fotini A.; Nikolaidis, Alexandros K.; Georgantea, Panagiota; Ioannou, Efstathia; Loupassaki, Sofia; Kefalas, Panagiotis; Kanellis, Angelos K.; Roussis, Vassilios; Makris, Antonios M.; Kampranis, Sotirios C.

I: Metabolic Engineering, Bind 27, 2015, s. 65-75.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ignea, C, Trikka, FA, Nikolaidis, AK, Georgantea, P, Ioannou, E, Loupassaki, S, Kefalas, P, Kanellis, AK, Roussis, V, Makris, AM & Kampranis, SC 2015, 'Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase', Metabolic Engineering, bind 27, s. 65-75. https://doi.org/10.1016/j.ymben.2014.10.008

APA

Ignea, C., Trikka, F. A., Nikolaidis, A. K., Georgantea, P., Ioannou, E., Loupassaki, S., Kefalas, P., Kanellis, A. K., Roussis, V., Makris, A. M., & Kampranis, S. C. (2015). Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase. Metabolic Engineering, 27, 65-75. https://doi.org/10.1016/j.ymben.2014.10.008

Vancouver

Ignea C, Trikka FA, Nikolaidis AK, Georgantea P, Ioannou E, Loupassaki S o.a. Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase. Metabolic Engineering. 2015;27:65-75. https://doi.org/10.1016/j.ymben.2014.10.008

Author

Ignea, Codruta ; Trikka, Fotini A. ; Nikolaidis, Alexandros K. ; Georgantea, Panagiota ; Ioannou, Efstathia ; Loupassaki, Sofia ; Kefalas, Panagiotis ; Kanellis, Angelos K. ; Roussis, Vassilios ; Makris, Antonios M. ; Kampranis, Sotirios C. / Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase. I: Metabolic Engineering. 2015 ; Bind 27. s. 65-75.

Bibtex

@article{2d7a9db9229544549d089c415456dc65,

title = "Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase",

abstract = "Terpenes have numerous applications, ranging from pharmaceuticals to fragrances and biofuels. With increasing interest in producing terpenes sustainably and economically, there has been significant progress in recent years in developing methods for their production in microorganisms. In Saccharomyces cerevisiae, production of the 20-carbon diterpenes has so far proven to be significantly less efficient than production of their 15-carbon sesquiterpene counterparts. In this report, we identify the modular structure of geranylgeranyl diphosphate synthesis in yeast to be a major limitation in diterpene yields, and we engineer the yeast farnesyl diphosphate synthase Erg20p to produce geranylgeranyl diphosphate. Using a combination of protein and genetic engineering, we achieve significant improvements in the production of sclareol and several other isoprenoids, including cis-abienol, abietadiene and β-carotene. We also report the development of yeast strains carrying the engineered Erg20p, which support efficient isoprenoid production and can be used as a dedicated chassis for diterpene production or biosynthetic pathway elucidation. The design developed here can be applied to the production of any GGPP-derived isoprenoid and is compatible with other yeast terpene production platforms.",

keywords = "Diterpenes, Geranyltranstransferase, Metabolic Engineering, Polyisoprenyl Phosphates, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins",

author = "Codruta Ignea and Trikka, {Fotini A.} and Nikolaidis, {Alexandros K.} and Panagiota Georgantea and Efstathia Ioannou and Sofia Loupassaki and Panagiotis Kefalas and Kanellis, {Angelos K.} and Vassilios Roussis and Makris, {Antonios M.} and Kampranis, {Sotirios C.}",

year = "2015",

doi = "10.1016/j.ymben.2014.10.008",

language = "English",

volume = "27",

pages = "65--75",

journal = "Metabolic Engineering",

issn = "1096-7176",

publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase

AU - Ignea, Codruta

AU - Trikka, Fotini A.

AU - Nikolaidis, Alexandros K.

AU - Georgantea, Panagiota

AU - Ioannou, Efstathia

AU - Loupassaki, Sofia

AU - Kefalas, Panagiotis

AU - Kanellis, Angelos K.

AU - Roussis, Vassilios

AU - Makris, Antonios M.

AU - Kampranis, Sotirios C.

PY - 2015

Y1 - 2015

N2 - Terpenes have numerous applications, ranging from pharmaceuticals to fragrances and biofuels. With increasing interest in producing terpenes sustainably and economically, there has been significant progress in recent years in developing methods for their production in microorganisms. In Saccharomyces cerevisiae, production of the 20-carbon diterpenes has so far proven to be significantly less efficient than production of their 15-carbon sesquiterpene counterparts. In this report, we identify the modular structure of geranylgeranyl diphosphate synthesis in yeast to be a major limitation in diterpene yields, and we engineer the yeast farnesyl diphosphate synthase Erg20p to produce geranylgeranyl diphosphate. Using a combination of protein and genetic engineering, we achieve significant improvements in the production of sclareol and several other isoprenoids, including cis-abienol, abietadiene and β-carotene. We also report the development of yeast strains carrying the engineered Erg20p, which support efficient isoprenoid production and can be used as a dedicated chassis for diterpene production or biosynthetic pathway elucidation. The design developed here can be applied to the production of any GGPP-derived isoprenoid and is compatible with other yeast terpene production platforms.

AB - Terpenes have numerous applications, ranging from pharmaceuticals to fragrances and biofuels. With increasing interest in producing terpenes sustainably and economically, there has been significant progress in recent years in developing methods for their production in microorganisms. In Saccharomyces cerevisiae, production of the 20-carbon diterpenes has so far proven to be significantly less efficient than production of their 15-carbon sesquiterpene counterparts. In this report, we identify the modular structure of geranylgeranyl diphosphate synthesis in yeast to be a major limitation in diterpene yields, and we engineer the yeast farnesyl diphosphate synthase Erg20p to produce geranylgeranyl diphosphate. Using a combination of protein and genetic engineering, we achieve significant improvements in the production of sclareol and several other isoprenoids, including cis-abienol, abietadiene and β-carotene. We also report the development of yeast strains carrying the engineered Erg20p, which support efficient isoprenoid production and can be used as a dedicated chassis for diterpene production or biosynthetic pathway elucidation. The design developed here can be applied to the production of any GGPP-derived isoprenoid and is compatible with other yeast terpene production platforms.

KW - Diterpenes

KW - Geranyltranstransferase

KW - Metabolic Engineering

KW - Polyisoprenyl Phosphates

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

U2 - 10.1016/j.ymben.2014.10.008

DO - 10.1016/j.ymben.2014.10.008

M3 - Journal article

C2 - 25446975

VL - 27

SP - 65

EP - 75

JO - Metabolic Engineering

JF - Metabolic Engineering

SN - 1096-7176

ER -

ID: 159084677