This protocol describes how to order and directly assemble uracil-containing non-clonal DNA
fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of
making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is
highly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained from
any synthesizing company. The protocol drastically reduces time and handling between receiving the
synthesized DNA fragments and transforming with vector and DNA fragment(s).