Cytosolic phosphofructokinases are important for sugar homeostasis in leaves of Arabidopsis thaliana
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Cytosolic phosphofructokinases are important for sugar homeostasis in leaves of Arabidopsis thaliana. / Perby, Laura Kathrine; Richter, Simon; Weber, Konrad; Hieber, Alina Johanna; Hess, Natalia; Crocoll, Christoph; Mogensen, Helle Kildal; Pribil, Mathias; Burow, Meike; Nielsen, Tom Hamborg; Mustroph, Angelika.
I: Annals of Botany, Bind 129, Nr. 1, 2022, s. 37-52.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Cytosolic phosphofructokinases are important for sugar homeostasis in leaves of Arabidopsis thaliana
AU - Perby, Laura Kathrine
AU - Richter, Simon
AU - Weber, Konrad
AU - Hieber, Alina Johanna
AU - Hess, Natalia
AU - Crocoll, Christoph
AU - Mogensen, Helle Kildal
AU - Pribil, Mathias
AU - Burow, Meike
AU - Nielsen, Tom Hamborg
AU - Mustroph, Angelika
N1 - © The Author(s) 2021. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
PY - 2022
Y1 - 2022
N2 - BACKGROUND AND AIMS: ATP-dependent phosphofructokinases (PFKs) catalyse phosphorylation of the carbon-1 position of fructose-6-phosphate, to form fructose-1,6-bisphosphate. In the cytosol, this is considered a key step in channelling carbon into glycolysis. Arabidopsis thaliana has seven genes encoding PFK isoforms, two chloroplastic and five cytosolic. This study focusses on the four major cytosolic isoforms of PFK in vegetative tissues of A. thaliana.METHODS: We have isolated homozygous knock-out individual mutants (pfk1, pfk3, pfk6, pfk7) and two double mutants (pfk1/7 and pfk3/6) and characterized their growth and metabolic phenotypes.KEY RESULTS: In contrast to single mutants and the double mutant pfk3/6 for the hypoxia-responsive isoforms, the double mutant pfk1/7 had reduced PFK activity and shows a clear visual and metabolic phenotype with reduced shoot growth, early flowering, and elevated hexose levels. This mutant also has an altered ratio of short/long aliphatic glucosinolates and an altered root-shoot distribution. Surprisingly, this mutant does not show any major changes in short-term carbon flux and in levels of hexose-phosphates.CONCLUSIONS: We conclude that the two isoforms PFK1 and PFK7 are important for sugar homeostasis in leaf metabolism and apparently source/sink relations in Arabidopsis, while PFK3 and PFK6 only play a minor role under normal growth conditions.
AB - BACKGROUND AND AIMS: ATP-dependent phosphofructokinases (PFKs) catalyse phosphorylation of the carbon-1 position of fructose-6-phosphate, to form fructose-1,6-bisphosphate. In the cytosol, this is considered a key step in channelling carbon into glycolysis. Arabidopsis thaliana has seven genes encoding PFK isoforms, two chloroplastic and five cytosolic. This study focusses on the four major cytosolic isoforms of PFK in vegetative tissues of A. thaliana.METHODS: We have isolated homozygous knock-out individual mutants (pfk1, pfk3, pfk6, pfk7) and two double mutants (pfk1/7 and pfk3/6) and characterized their growth and metabolic phenotypes.KEY RESULTS: In contrast to single mutants and the double mutant pfk3/6 for the hypoxia-responsive isoforms, the double mutant pfk1/7 had reduced PFK activity and shows a clear visual and metabolic phenotype with reduced shoot growth, early flowering, and elevated hexose levels. This mutant also has an altered ratio of short/long aliphatic glucosinolates and an altered root-shoot distribution. Surprisingly, this mutant does not show any major changes in short-term carbon flux and in levels of hexose-phosphates.CONCLUSIONS: We conclude that the two isoforms PFK1 and PFK7 are important for sugar homeostasis in leaf metabolism and apparently source/sink relations in Arabidopsis, while PFK3 and PFK6 only play a minor role under normal growth conditions.
U2 - 10.1093/aob/mcab122
DO - 10.1093/aob/mcab122
M3 - Journal article
C2 - 34549262
VL - 129
SP - 37
EP - 52
JO - Annals of Botany
JF - Annals of Botany
SN - 0305-7364
IS - 1
ER -
ID: 283149950