Conidia-based fluorescence quantification of Streptomyces

Institut for Plante- og Miljøvidenskab

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Conidia-based fluorescence quantification of Streptomyces. / Podduturi, Raju; Jørgensen, Niels O. G.

I: Journal of Microbiological Methods, Bind 153, 2018, s. 104-107.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Podduturi, R & Jørgensen, NOG 2018, 'Conidia-based fluorescence quantification of Streptomyces', Journal of Microbiological Methods, bind 153, s. 104-107. https://doi.org/10.1016/j.mimet.2018.09.010

APA

Podduturi, R., & Jørgensen, N. O. G. (2018). Conidia-based fluorescence quantification of Streptomyces. Journal of Microbiological Methods, 153, 104-107. https://doi.org/10.1016/j.mimet.2018.09.010

Vancouver

Podduturi R, Jørgensen NOG. Conidia-based fluorescence quantification of Streptomyces. Journal of Microbiological Methods. 2018;153:104-107. https://doi.org/10.1016/j.mimet.2018.09.010

Author

Podduturi, Raju ; Jørgensen, Niels O. G. / Conidia-based fluorescence quantification of Streptomyces. I: Journal of Microbiological Methods. 2018 ; Bind 153. s. 104-107.

Bibtex

@article{1363b857d3514752a6a15f3dac496311,

title = "Conidia-based fluorescence quantification of Streptomyces",

abstract = "Determination of cell numbers in filamentous bacteria, such as Streptomyces, is challenging due to the tangled and twisted structure of the filaments and formation of cell clumps in liquid cultures. Here, we developed a conidia-based approach, in which fluorescence of conidia, after staining with the DNA-binding stain SYBR Green 1, was related to SYBR Green 1 fluorescence of DNA in Streptomyces. When cell number in Streptomyces filaments, determined by the conidia assay, was compared to number obtained by a qPCR assay, 34 to 62% of cells in the Streptomyces filaments were recovered. The difference in numbers probably reflects an insufficient extraction of DNA from the Gram-positive bacteria, rather than underestimation of the actual cell number by the conidia-based determination. The conidia-based approach appears to be a fast and reliable procedure for counting cell numbers in Streptomyces filaments but it can also be used for other filamentous bacteria, if proper standard curves can be made.",

author = "Raju Podduturi and J{\o}rgensen, {Niels O. G.}",

year = "2018",

doi = "10.1016/j.mimet.2018.09.010",

language = "English",

volume = "153",

pages = "104--107",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Conidia-based fluorescence quantification of Streptomyces

AU - Podduturi, Raju

AU - Jørgensen, Niels O. G.

PY - 2018

Y1 - 2018

N2 - Determination of cell numbers in filamentous bacteria, such as Streptomyces, is challenging due to the tangled and twisted structure of the filaments and formation of cell clumps in liquid cultures. Here, we developed a conidia-based approach, in which fluorescence of conidia, after staining with the DNA-binding stain SYBR Green 1, was related to SYBR Green 1 fluorescence of DNA in Streptomyces. When cell number in Streptomyces filaments, determined by the conidia assay, was compared to number obtained by a qPCR assay, 34 to 62% of cells in the Streptomyces filaments were recovered. The difference in numbers probably reflects an insufficient extraction of DNA from the Gram-positive bacteria, rather than underestimation of the actual cell number by the conidia-based determination. The conidia-based approach appears to be a fast and reliable procedure for counting cell numbers in Streptomyces filaments but it can also be used for other filamentous bacteria, if proper standard curves can be made.

AB - Determination of cell numbers in filamentous bacteria, such as Streptomyces, is challenging due to the tangled and twisted structure of the filaments and formation of cell clumps in liquid cultures. Here, we developed a conidia-based approach, in which fluorescence of conidia, after staining with the DNA-binding stain SYBR Green 1, was related to SYBR Green 1 fluorescence of DNA in Streptomyces. When cell number in Streptomyces filaments, determined by the conidia assay, was compared to number obtained by a qPCR assay, 34 to 62% of cells in the Streptomyces filaments were recovered. The difference in numbers probably reflects an insufficient extraction of DNA from the Gram-positive bacteria, rather than underestimation of the actual cell number by the conidia-based determination. The conidia-based approach appears to be a fast and reliable procedure for counting cell numbers in Streptomyces filaments but it can also be used for other filamentous bacteria, if proper standard curves can be made.

U2 - 10.1016/j.mimet.2018.09.010

DO - 10.1016/j.mimet.2018.09.010

M3 - Journal article

C2 - 30244124

VL - 153

SP - 104

EP - 107

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

ER -

ID: 204473955