The hybrid origin of two cultivars of crocus (Iridaceae) analysed by molecular cytogenetics including genomic southern and in situ hybridization

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The hybrid origin of two cultivars of crocus (Iridaceae) analysed by molecular cytogenetics including genomic southern and in situ hybridization. / Ørgaard, M.; Jacobsen, N.; Heslop-Harrison, J. S.

I: Annals of Botany, Bind 76, Nr. 3, 09.1995, s. 253-262.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ørgaard, M, Jacobsen, N & Heslop-Harrison, JS 1995, 'The hybrid origin of two cultivars of crocus (Iridaceae) analysed by molecular cytogenetics including genomic southern and in situ hybridization', Annals of Botany, bind 76, nr. 3, s. 253-262. https://doi.org/10.1006/anbo.1995.1094

APA

Ørgaard, M., Jacobsen, N., & Heslop-Harrison, J. S. (1995). The hybrid origin of two cultivars of crocus (Iridaceae) analysed by molecular cytogenetics including genomic southern and in situ hybridization. Annals of Botany, 76(3), 253-262. https://doi.org/10.1006/anbo.1995.1094

Vancouver

Ørgaard M, Jacobsen N, Heslop-Harrison JS. The hybrid origin of two cultivars of crocus (Iridaceae) analysed by molecular cytogenetics including genomic southern and in situ hybridization. Annals of Botany. 1995 sep.;76(3):253-262. https://doi.org/10.1006/anbo.1995.1094

Author

Ørgaard, M. ; Jacobsen, N. ; Heslop-Harrison, J. S. / The hybrid origin of two cultivars of crocus (Iridaceae) analysed by molecular cytogenetics including genomic southern and in situ hybridization. I: Annals of Botany. 1995 ; Bind 76, Nr. 3. s. 253-262.

Bibtex

@article{3dde2761225f46a98f9f77dada57b003,
title = "The hybrid origin of two cultivars of crocus (Iridaceae) analysed by molecular cytogenetics including genomic southern and in situ hybridization",
abstract = "The origin of the two common cultivars of Crocus, C. {\textquoteleft}Stellaris{\textquoteright} (2n = 2x = 10) and C. {\textquoteleft}Golden Yellow{\textquoteright} (2n = 3x = 14) was investigated by fluorescent in situ hybridization using both total genomic DNA and cloned DNA sequences as probes. The clear differentiation between the chromosomes after genomic in situ hybridization supports the proposals of a hybrid origin of the cultivars and shows that they have the same parental genomes originating from C. flavus (2n = 8) and C. angustifolius (2n = 12). C. {\textquoteleft}Stellaris{\textquoteright} has four chromosomes of C. flavus origin and six chromosomes of C. angustifolius origin. C. {\textquoteleft}Golden Yellow{\textquoteright} has eight chromosomes of C. flavus origin and six chromosomes of C. angustifolius origin. The number and location of 18S-5·8S-26S rRNA genes on the chromosomes of the hybrids and of the parental species agree with the results from the genomic probings. Hybridization to Southern membranes also supports the hybrid origin of C. {\textquoteleft}Golden Yellow{\textquoteright}.",
keywords = "Crocus, Cytology, In situ hybridization, RDNA sites, Southern hybridization, Taxonomy",
author = "M. {\O}rgaard and N. Jacobsen and Heslop-Harrison, {J. S.}",
year = "1995",
month = sep,
doi = "10.1006/anbo.1995.1094",
language = "English",
volume = "76",
pages = "253--262",
journal = "Annals of Botany",
issn = "0305-7364",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - The hybrid origin of two cultivars of crocus (Iridaceae) analysed by molecular cytogenetics including genomic southern and in situ hybridization

AU - Ørgaard, M.

AU - Jacobsen, N.

AU - Heslop-Harrison, J. S.

PY - 1995/9

Y1 - 1995/9

N2 - The origin of the two common cultivars of Crocus, C. ‘Stellaris’ (2n = 2x = 10) and C. ‘Golden Yellow’ (2n = 3x = 14) was investigated by fluorescent in situ hybridization using both total genomic DNA and cloned DNA sequences as probes. The clear differentiation between the chromosomes after genomic in situ hybridization supports the proposals of a hybrid origin of the cultivars and shows that they have the same parental genomes originating from C. flavus (2n = 8) and C. angustifolius (2n = 12). C. ‘Stellaris’ has four chromosomes of C. flavus origin and six chromosomes of C. angustifolius origin. C. ‘Golden Yellow’ has eight chromosomes of C. flavus origin and six chromosomes of C. angustifolius origin. The number and location of 18S-5·8S-26S rRNA genes on the chromosomes of the hybrids and of the parental species agree with the results from the genomic probings. Hybridization to Southern membranes also supports the hybrid origin of C. ‘Golden Yellow’.

AB - The origin of the two common cultivars of Crocus, C. ‘Stellaris’ (2n = 2x = 10) and C. ‘Golden Yellow’ (2n = 3x = 14) was investigated by fluorescent in situ hybridization using both total genomic DNA and cloned DNA sequences as probes. The clear differentiation between the chromosomes after genomic in situ hybridization supports the proposals of a hybrid origin of the cultivars and shows that they have the same parental genomes originating from C. flavus (2n = 8) and C. angustifolius (2n = 12). C. ‘Stellaris’ has four chromosomes of C. flavus origin and six chromosomes of C. angustifolius origin. C. ‘Golden Yellow’ has eight chromosomes of C. flavus origin and six chromosomes of C. angustifolius origin. The number and location of 18S-5·8S-26S rRNA genes on the chromosomes of the hybrids and of the parental species agree with the results from the genomic probings. Hybridization to Southern membranes also supports the hybrid origin of C. ‘Golden Yellow’.

KW - Crocus

KW - Cytology

KW - In situ hybridization

KW - RDNA sites

KW - Southern hybridization

KW - Taxonomy

UR - http://www.scopus.com/inward/record.url?scp=58149363697&partnerID=8YFLogxK

U2 - 10.1006/anbo.1995.1094

DO - 10.1006/anbo.1995.1094

M3 - Journal article

AN - SCOPUS:58149363697

VL - 76

SP - 253

EP - 262

JO - Annals of Botany

JF - Annals of Botany

SN - 0305-7364

IS - 3

ER -

ID: 322659493