Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana: Expression patterns, location and stress responses
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Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana : Expression patterns, location and stress responses. / Grabsztunowicz, Magda; Rantala, Marjaana; Ivanauskaite, Aiste; Blomster, Tiina; Koskela, Minna M.; Vuorinen, Katariina; Tyystjarvi, Esa; Burow, Meike; Overmyer, Kirk; Mahonen, Ari P.; Mulo, Paula.
I: Plant, Cell and Environment, Bind 44, Nr. 2, 2021, s. 548-558.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana
T2 - Expression patterns, location and stress responses
AU - Grabsztunowicz, Magda
AU - Rantala, Marjaana
AU - Ivanauskaite, Aiste
AU - Blomster, Tiina
AU - Koskela, Minna M.
AU - Vuorinen, Katariina
AU - Tyystjarvi, Esa
AU - Burow, Meike
AU - Overmyer, Kirk
AU - Mahonen, Ari P.
AU - Mulo, Paula
PY - 2021
Y1 - 2021
N2 - In Arabidopsis, two leaf-type ferredoxin-NADP(+) oxidoreductase (LFNR) isoforms function in photosynthetic electron flow in reduction of NADP(+), while two root-type FNR (RFNR) isoforms catalyse reduction of ferredoxin in non-photosynthetic plastids. As the key to understanding, the function of RFNRs might lie in their spatial and temporal distribution in different plant tissues and cell types, we examined expression of RFNR1 and RFNR2 genes using beta-glucuronidase (GUS) reporter lines and investigated accumulation of distinct RFNR isoforms using a GFP approach and Western blotting upon various stresses. We show that while RFNR1 promoter is active in leaf veins, root tips and in the stele of roots, RFNR2 promoter activity is present in leaf tips and root stele, epidermis and cortex. RFNR1 protein accumulates as a soluble protein within the plastids of root stele cells, while RFNR2 is mainly present in the outer root layers. Ozone treatment of plants enhanced accumulation of RFNR1, whereas low temperature treatment specifically affected RFNR2 accumulation in roots. We further discuss the physiological roles of RFNR1 and RFNR2 based on characterization of rfnr1 and rfnr2 knock-out plants and show that although the function of these proteins is partly redundant, the RFNR proteins are essential for plant development and survival.
AB - In Arabidopsis, two leaf-type ferredoxin-NADP(+) oxidoreductase (LFNR) isoforms function in photosynthetic electron flow in reduction of NADP(+), while two root-type FNR (RFNR) isoforms catalyse reduction of ferredoxin in non-photosynthetic plastids. As the key to understanding, the function of RFNRs might lie in their spatial and temporal distribution in different plant tissues and cell types, we examined expression of RFNR1 and RFNR2 genes using beta-glucuronidase (GUS) reporter lines and investigated accumulation of distinct RFNR isoforms using a GFP approach and Western blotting upon various stresses. We show that while RFNR1 promoter is active in leaf veins, root tips and in the stele of roots, RFNR2 promoter activity is present in leaf tips and root stele, epidermis and cortex. RFNR1 protein accumulates as a soluble protein within the plastids of root stele cells, while RFNR2 is mainly present in the outer root layers. Ozone treatment of plants enhanced accumulation of RFNR1, whereas low temperature treatment specifically affected RFNR2 accumulation in roots. We further discuss the physiological roles of RFNR1 and RFNR2 based on characterization of rfnr1 and rfnr2 knock-out plants and show that although the function of these proteins is partly redundant, the RFNR proteins are essential for plant development and survival.
KW - Arabidopsis
KW - ferredoxin‐
KW - NADP(+) oxidoreductase
KW - gene expression
KW - low temperature
KW - ozone
KW - plastid
KW - root
KW - stress response
KW - NADP+-OXIDOREDUCTASE
KW - THYLAKOID MEMBRANES
KW - GLUTAMATE SYNTHASE
KW - NITRITE REDUCTION
KW - ELECTRON-TRANSFER
KW - DIFFERENT ORGANS
KW - PLANTS
KW - FNR
KW - PURIFICATION
KW - COMPLEXES
U2 - 10.1111/pce.13932
DO - 10.1111/pce.13932
M3 - Journal article
C2 - 33131061
VL - 44
SP - 548
EP - 558
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
SN - 0140-7791
IS - 2
ER -
ID: 252470795