Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters
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Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters. / Nour-Eldin, Hussam Hassan; Madsen, Svend Roesen; Engelen, Steven; Jørgensen, Morten Egevang; Olsen, Carl Erik; Andersen, Jonathan Sonne; Seynnaeve, David; Verhoye, Thalia; Fulawka, Rudy; Denolf, Peter; Halkier, Barbara Ann.
I: Nature Biotechnology, Bind 35, Nr. 4, 2017, s. 377-382.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters
AU - Nour-Eldin, Hussam Hassan
AU - Madsen, Svend Roesen
AU - Engelen, Steven
AU - Jørgensen, Morten Egevang
AU - Olsen, Carl Erik
AU - Andersen, Jonathan Sonne
AU - Seynnaeve, David
AU - Verhoye, Thalia
AU - Fulawka, Rudy
AU - Denolf, Peter
AU - Halkier, Barbara Ann
PY - 2017
Y1 - 2017
N2 - The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.
AB - The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.
KW - Brassica
KW - Genetic Enhancement
KW - Glucosinolates
KW - Monosaccharide Transport Proteins
KW - Mutation
KW - Plant Oils
KW - Plants, Genetically Modified
KW - Seeds
KW - Journal Article
U2 - 10.1038/nbt.3823
DO - 10.1038/nbt.3823
M3 - Journal article
C2 - 28288105
VL - 35
SP - 377
EP - 382
JO - Nature Biotechnology
JF - Nature Biotechnology
SN - 1087-0156
IS - 4
ER -
ID: 180759552