Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development
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Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development. / Guo, Xiaoyuan; Runavot, Jean Luc; Bourot, Stéphane; Meulewaeter, Frank; Hernandez-Gomez, Mercedes; Holland, Claire; Harholt, Jesper; Willats, William G.T.; Mravec, Jozef; Knox, Paul; Ulvskov, Peter.
I: Planta, Bind 249, Nr. 5, 05.2019, s. 1565–1581.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development
AU - Guo, Xiaoyuan
AU - Runavot, Jean Luc
AU - Bourot, Stéphane
AU - Meulewaeter, Frank
AU - Hernandez-Gomez, Mercedes
AU - Holland, Claire
AU - Harholt, Jesper
AU - Willats, William G.T.
AU - Mravec, Jozef
AU - Knox, Paul
AU - Ulvskov, Peter
PY - 2019/5
Y1 - 2019/5
N2 - Main conclusion: Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.
AB - Main conclusion: Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.
KW - Arabinofuranosidase
KW - Cuticle
KW - Post-genital fusion
KW - Rhamnogalacturonan-I
KW - Xyloglucan
U2 - 10.1007/s00425-019-03107-4
DO - 10.1007/s00425-019-03107-4
M3 - Journal article
C2 - 30737556
AN - SCOPUS:85061293221
VL - 249
SP - 1565
EP - 1581
JO - Planta
JF - Planta
SN - 0032-0935
IS - 5
ER -
ID: 216214923