Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction of Colletotrichum sublineolum in sorghum

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Standard

Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction of Colletotrichum sublineolum in sorghum. / Basavaraju, Puttalingaiah; Shailasree, Shekar; Shetty, Nandini P.; Kini, Ramachandra K.; Jørgensen, Hans J.L.; de Neergaard, Eigil; Shetty, Hunthrike S.

I: Journal of Plant Interactions, Bind 4, Nr. 3, 2009, s. 179-186.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Basavaraju, P, Shailasree, S, Shetty, NP, Kini, RK, Jørgensen, HJL, de Neergaard, E & Shetty, HS 2009, 'Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction of Colletotrichum sublineolum in sorghum', Journal of Plant Interactions, bind 4, nr. 3, s. 179-186. https://doi.org/10.1080/17429140802527169

APA

Basavaraju, P., Shailasree, S., Shetty, N. P., Kini, R. K., Jørgensen, H. J. L., de Neergaard, E., & Shetty, H. S. (2009). Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction of Colletotrichum sublineolum in sorghum. Journal of Plant Interactions, 4(3), 179-186. https://doi.org/10.1080/17429140802527169

Vancouver

Basavaraju P, Shailasree S, Shetty NP, Kini RK, Jørgensen HJL, de Neergaard E o.a. Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction of Colletotrichum sublineolum in sorghum. Journal of Plant Interactions. 2009;4(3):179-186. https://doi.org/10.1080/17429140802527169

Author

Basavaraju, Puttalingaiah ; Shailasree, Shekar ; Shetty, Nandini P. ; Kini, Ramachandra K. ; Jørgensen, Hans J.L. ; de Neergaard, Eigil ; Shetty, Hunthrike S. / Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction of Colletotrichum sublineolum in sorghum. I: Journal of Plant Interactions. 2009 ; Bind 4, Nr. 3. s. 179-186.

Bibtex

@article{964c6aed9f1b4ee69e69f1dba30378f8,

title = "Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction of Colletotrichum sublineolum in sorghum",

abstract = "Hydroxyproline-rich glycoproteins (HRGPs) accumulation and oxidative cross-linking is one of the earliest defense responses in plants against pathogen infection. In the present study HRGP accumulation in three sorghum genotypes i.e. SC146 (resistant), SC326 (intermediately resistant) and BTx623 (susceptible) as a response to Colletotrichum sublineolum isolate CP2126 infection is elucidated. HRGPs were monitored by hydroxyproline (Hyp) estimation. In genotypes SC146 and SC326 there was a significantly higher amounts of Hyp at 2 days after inoculation (dai) compared to genotype BTx623, indicating an infection induced accumulation of HRGPs. Western blot analysis of acid-ethanol extracted proteins with polyclonal antibody raised against pearl millet purified HRGPs identified four bands with molecular masses of ∼65, 45, 17 and 14 kDa as HRGPs. Insolubilization of the 45 kDa protein in genotypes SC146 and SC326 upon infection with C. sublineolum indicates a role of this protein in cell wall cross-linking, coinciding with heavier accumulation of hydrogen peroxide. In addition, tissue print analysis using polyclonal antibody of pearl millet HRGPs recognized these cross-linked proteins to be HRGPs. These findings indicated that HRGPs in sorghum is a component of defense reaction against C. sublineolum infection.",

keywords = "Disease resistance, Hydrogen peroxide, Hydroxyproline, Hydroxyproline-rich glycoproteins, Sorghum anthracnose",

author = "Puttalingaiah Basavaraju and Shekar Shailasree and Shetty, {Nandini P.} and Kini, {Ramachandra K.} and J{\o}rgensen, {Hans J.L.} and {de Neergaard}, Eigil and Shetty, {Hunthrike S.}",

year = "2009",

doi = "10.1080/17429140802527169",

language = "English",

volume = "4",

pages = "179--186",

journal = "Journal of Plant Interactions",

issn = "1742-9145",

publisher = "Taylor & Francis",

number = "3",

}

RIS

TY - JOUR

T1 - Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction of Colletotrichum sublineolum in sorghum

AU - Basavaraju, Puttalingaiah

AU - Shailasree, Shekar

AU - Shetty, Nandini P.

AU - Kini, Ramachandra K.

AU - Jørgensen, Hans J.L.

AU - de Neergaard, Eigil

AU - Shetty, Hunthrike S.

PY - 2009

Y1 - 2009

N2 - Hydroxyproline-rich glycoproteins (HRGPs) accumulation and oxidative cross-linking is one of the earliest defense responses in plants against pathogen infection. In the present study HRGP accumulation in three sorghum genotypes i.e. SC146 (resistant), SC326 (intermediately resistant) and BTx623 (susceptible) as a response to Colletotrichum sublineolum isolate CP2126 infection is elucidated. HRGPs were monitored by hydroxyproline (Hyp) estimation. In genotypes SC146 and SC326 there was a significantly higher amounts of Hyp at 2 days after inoculation (dai) compared to genotype BTx623, indicating an infection induced accumulation of HRGPs. Western blot analysis of acid-ethanol extracted proteins with polyclonal antibody raised against pearl millet purified HRGPs identified four bands with molecular masses of ∼65, 45, 17 and 14 kDa as HRGPs. Insolubilization of the 45 kDa protein in genotypes SC146 and SC326 upon infection with C. sublineolum indicates a role of this protein in cell wall cross-linking, coinciding with heavier accumulation of hydrogen peroxide. In addition, tissue print analysis using polyclonal antibody of pearl millet HRGPs recognized these cross-linked proteins to be HRGPs. These findings indicated that HRGPs in sorghum is a component of defense reaction against C. sublineolum infection.

AB - Hydroxyproline-rich glycoproteins (HRGPs) accumulation and oxidative cross-linking is one of the earliest defense responses in plants against pathogen infection. In the present study HRGP accumulation in three sorghum genotypes i.e. SC146 (resistant), SC326 (intermediately resistant) and BTx623 (susceptible) as a response to Colletotrichum sublineolum isolate CP2126 infection is elucidated. HRGPs were monitored by hydroxyproline (Hyp) estimation. In genotypes SC146 and SC326 there was a significantly higher amounts of Hyp at 2 days after inoculation (dai) compared to genotype BTx623, indicating an infection induced accumulation of HRGPs. Western blot analysis of acid-ethanol extracted proteins with polyclonal antibody raised against pearl millet purified HRGPs identified four bands with molecular masses of ∼65, 45, 17 and 14 kDa as HRGPs. Insolubilization of the 45 kDa protein in genotypes SC146 and SC326 upon infection with C. sublineolum indicates a role of this protein in cell wall cross-linking, coinciding with heavier accumulation of hydrogen peroxide. In addition, tissue print analysis using polyclonal antibody of pearl millet HRGPs recognized these cross-linked proteins to be HRGPs. These findings indicated that HRGPs in sorghum is a component of defense reaction against C. sublineolum infection.

KW - Disease resistance

KW - Hydrogen peroxide

KW - Hydroxyproline

KW - Hydroxyproline-rich glycoproteins

KW - Sorghum anthracnose

U2 - 10.1080/17429140802527169

DO - 10.1080/17429140802527169

M3 - Journal article

AN - SCOPUS:70349690465

VL - 4

SP - 179

EP - 186

JO - Journal of Plant Interactions

JF - Journal of Plant Interactions

SN - 1742-9145

IS - 3

ER -

ID: 203519961