Discovery of a bacterial gene cluster for deglycosylation of the toxic potato steroidal glycoalkaloids α-chaconine and α-solanine

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Standard

Discovery of a bacterial gene cluster for deglycosylation of the toxic potato steroidal glycoalkaloids α-chaconine and α-solanine. / Hennessy, Rosanna Catherine; Nielsen, Søren D.; Greve-Poulsen, Mathias; Larsen, Lotte Bach; Bandsholm, Ole; Stougaard, Peter.

I: Journal of Agricultural and Food Chemistry, Bind 68, Nr. 5, 2020, s. 1390-1396.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Hennessy, RC, Nielsen, SD, Greve-Poulsen, M, Larsen, LB, Bandsholm, O & Stougaard, P 2020, 'Discovery of a bacterial gene cluster for deglycosylation of the toxic potato steroidal glycoalkaloids α-chaconine and α-solanine', Journal of Agricultural and Food Chemistry, bind 68, nr. 5, s. 1390-1396. https://doi.org/10.1021/acs.jafc.9b07632

APA

Hennessy, R. C., Nielsen, S. D., Greve-Poulsen, M., Larsen, L. B., Bandsholm, O., & Stougaard, P. (2020). Discovery of a bacterial gene cluster for deglycosylation of the toxic potato steroidal glycoalkaloids α-chaconine and α-solanine. Journal of Agricultural and Food Chemistry, 68(5), 1390-1396. https://doi.org/10.1021/acs.jafc.9b07632

Vancouver

Hennessy RC, Nielsen SD, Greve-Poulsen M, Larsen LB, Bandsholm O, Stougaard P. Discovery of a bacterial gene cluster for deglycosylation of the toxic potato steroidal glycoalkaloids α-chaconine and α-solanine. Journal of Agricultural and Food Chemistry. 2020;68(5):1390-1396. https://doi.org/10.1021/acs.jafc.9b07632

Author

Hennessy, Rosanna Catherine ; Nielsen, Søren D. ; Greve-Poulsen, Mathias ; Larsen, Lotte Bach ; Bandsholm, Ole ; Stougaard, Peter. / Discovery of a bacterial gene cluster for deglycosylation of the toxic potato steroidal glycoalkaloids α-chaconine and α-solanine. I: Journal of Agricultural and Food Chemistry. 2020 ; Bind 68, Nr. 5. s. 1390-1396.

Bibtex

@article{fd6d5c7e0dbf48f9b244fce44ad86075,
title = "Discovery of a bacterial gene cluster for deglycosylation of the toxic potato steroidal glycoalkaloids α-chaconine and α-solanine",
abstract = "Potato juice is a by-product of starch processing currently used as feed. However, potato proteins are an untapped source of high-protein food for human nutrition if harmful constituents notably glycoalkaloids (GAs) are detoxified. The two principle GAs found in potato are α-chaconine and α-solanine, both consisting of a solanidine aglycone with a carbohydrate side-chain. The first step in the detoxification of these compounds is removal of the trisaccharide. Whole-genome sequencing of a bacterial isolate, Arthrobacter sp. S41, that degrade completely α-chaconine and α-solanine revealed the presence of a gene cluster possibly involved in the deglycosylation of GAs. Functional characterization confirmed the enzymatic activity of the gene cluster involved in the complete deglycosylation of both α-chaconine and α-solanine. The novel enzymes described here may find value in the bioconversion of feed proteins to food proteins suitable for human nutrition.",
author = "Hennessy, {Rosanna Catherine} and Nielsen, {S{\o}ren D.} and Mathias Greve-Poulsen and Larsen, {Lotte Bach} and Ole Bandsholm and Peter Stougaard",
year = "2020",
doi = "10.1021/acs.jafc.9b07632",
language = "English",
volume = "68",
pages = "1390--1396",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "5",

}

RIS

TY - JOUR

T1 - Discovery of a bacterial gene cluster for deglycosylation of the toxic potato steroidal glycoalkaloids α-chaconine and α-solanine

AU - Hennessy, Rosanna Catherine

AU - Nielsen, Søren D.

AU - Greve-Poulsen, Mathias

AU - Larsen, Lotte Bach

AU - Bandsholm, Ole

AU - Stougaard, Peter

PY - 2020

Y1 - 2020

N2 - Potato juice is a by-product of starch processing currently used as feed. However, potato proteins are an untapped source of high-protein food for human nutrition if harmful constituents notably glycoalkaloids (GAs) are detoxified. The two principle GAs found in potato are α-chaconine and α-solanine, both consisting of a solanidine aglycone with a carbohydrate side-chain. The first step in the detoxification of these compounds is removal of the trisaccharide. Whole-genome sequencing of a bacterial isolate, Arthrobacter sp. S41, that degrade completely α-chaconine and α-solanine revealed the presence of a gene cluster possibly involved in the deglycosylation of GAs. Functional characterization confirmed the enzymatic activity of the gene cluster involved in the complete deglycosylation of both α-chaconine and α-solanine. The novel enzymes described here may find value in the bioconversion of feed proteins to food proteins suitable for human nutrition.

AB - Potato juice is a by-product of starch processing currently used as feed. However, potato proteins are an untapped source of high-protein food for human nutrition if harmful constituents notably glycoalkaloids (GAs) are detoxified. The two principle GAs found in potato are α-chaconine and α-solanine, both consisting of a solanidine aglycone with a carbohydrate side-chain. The first step in the detoxification of these compounds is removal of the trisaccharide. Whole-genome sequencing of a bacterial isolate, Arthrobacter sp. S41, that degrade completely α-chaconine and α-solanine revealed the presence of a gene cluster possibly involved in the deglycosylation of GAs. Functional characterization confirmed the enzymatic activity of the gene cluster involved in the complete deglycosylation of both α-chaconine and α-solanine. The novel enzymes described here may find value in the bioconversion of feed proteins to food proteins suitable for human nutrition.

U2 - 10.1021/acs.jafc.9b07632

DO - 10.1021/acs.jafc.9b07632

M3 - Journal article

C2 - 31935098

VL - 68

SP - 1390

EP - 1396

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 5

ER -

ID: 234346842