De novo production of benzyl glucosinolate in Escherichia coli
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De novo production of benzyl glucosinolate in Escherichia coli. / Petersen, Annette; Crocoll, Christoph; Halkier, Barbara Ann.
I: Metabolic Engineering, Bind 54, 01.07.2019, s. 24-34.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - De novo production of benzyl glucosinolate in Escherichia coli
AU - Petersen, Annette
AU - Crocoll, Christoph
AU - Halkier, Barbara Ann
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Microbial production of plant specialised metabolites is challenging as the biosynthetic pathways are often complex and can contain enzymes, which function is not supported in traditional production hosts. Glucosinolates are specialised metabolites of strong commercial interest due to their health-promoting effects. In this work, we engineered the production of benzyl glucosinolate in Escherichia coli. We systematically optimised the production levels by first screening different expression strains and by modification of growth conditions and media compositions. This resulted in production from undetectable to approximately 4.1 μM benzyl glucosinolate, but also approximately 3.7 μM of desulfo-benzyl glucosinolate, the final intermediate of this pathway. Additional optimisation of pathway flux through entry point cytochrome P450 enzymes and PAPS-dependent sulfotransferase increased the production additionally 5-fold to 20.3 μM (equivalent to 8.3 mg/L) benzyl glucosinolate.
AB - Microbial production of plant specialised metabolites is challenging as the biosynthetic pathways are often complex and can contain enzymes, which function is not supported in traditional production hosts. Glucosinolates are specialised metabolites of strong commercial interest due to their health-promoting effects. In this work, we engineered the production of benzyl glucosinolate in Escherichia coli. We systematically optimised the production levels by first screening different expression strains and by modification of growth conditions and media compositions. This resulted in production from undetectable to approximately 4.1 μM benzyl glucosinolate, but also approximately 3.7 μM of desulfo-benzyl glucosinolate, the final intermediate of this pathway. Additional optimisation of pathway flux through entry point cytochrome P450 enzymes and PAPS-dependent sulfotransferase increased the production additionally 5-fold to 20.3 μM (equivalent to 8.3 mg/L) benzyl glucosinolate.
KW - Escherichia coli
KW - Glucosinolates
KW - Metabolic engineering
KW - Targeted proteomics
UR - http://www.scopus.com/inward/record.url?scp=85063026606&partnerID=8YFLogxK
U2 - 10.1016/j.ymben.2019.02.004
DO - 10.1016/j.ymben.2019.02.004
M3 - Journal article
C2 - 30831267
AN - SCOPUS:85063026606
VL - 54
SP - 24
EP - 34
JO - Metabolic Engineering
JF - Metabolic Engineering
SN - 1096-7176
ER -
ID: 223678215