P-type Pumps – University of Copenhagen

Pumps in cells

We study the structure, function and regulation of primary active transport across membranes. Our major focus is P-type ATPase pumps that form a large superfamily in all forms of life. P-type ATPases pump cations (like essential metals, calcium and protons) and phospholipids across membranes. Well-characterized members are essential for many basic functions in cells and we aim at assigning physiological function to other less characterized pumps. All members of this family form a phosphorylated reaction cycle intermediate, hence the name P-type, and their evolution raises unanswered questions that we try to answer. The pumping mechanism of these biological nanomachines and how pumping is regulated is also investigated.

Selected publications

  • Hoffmann RD, Palmgren MG (2013) Epigenetic repression of male gametophyte-specific genes in the Arabidopsis sporophyte. Mol. Plant 6: 1176-86.
  • Tidow H, Poulsen LR, Andreeva A, Knudsen M, Hein KL, Wiuf C, Palmgren MG, Nissen P (2012) A bimodular mechanism of calcium control in eukaryotes. Nature 491: 468-72.
  • Mikkelsen MD, Pedas P, Schiller M, Vincze E, Mills RF, Borg S, Møller A, Schjoerring JK, Williams LE, Baekgaard L, Holm PB, Palmgren MG (2012) Barley HvHMA1 is a heavy metal pump involved in mo-bilizing organellar Zn and Cu and plays a role in metal loading into grains. PLoS One 7(11): e49027.
  • Palmgren MG, Nissen P (2011) P-type ATPases. Annu. Rev. Biophys. 40: 243–66.
  • Ekberg K, Pedersen BP, Sørensen DM, Nielsen AK, Veierskov B, Nissen P, Palmgren MG, Buch-Pedersen MJ (2010) Structural identification of cation binding pockets in the plasma membrane proton pump. Proc. Natl. Acad. Sci. U.S.A. 107: 21400-21405.