Viability of Ascaris suum eggs in stored raw and separated liquid slurry

Research output: Contribution to journalJournal articleResearchpeer-review

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Viability of Ascaris suum eggs in stored raw and separated liquid slurry. / Katakam, Kiran Kumar; Roepstorff, Allan Knud; Popovic, Olga; Kyvsgaard, Niels Christian; Thamsborg, Stig Milan; Dalsgaard, Anders.

In: Parasitology, Vol. 140, No. 3, 2013, p. 378-384.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Katakam, KK, Roepstorff, AK, Popovic, O, Kyvsgaard, NC, Thamsborg, SM & Dalsgaard, A 2013, 'Viability of Ascaris suum eggs in stored raw and separated liquid slurry', Parasitology, vol. 140, no. 3, pp. 378-384. https://doi.org/10.1017/S0031182012001722

APA

Katakam, K. K., Roepstorff, A. K., Popovic, O., Kyvsgaard, N. C., Thamsborg, S. M., & Dalsgaard, A. (2013). Viability of Ascaris suum eggs in stored raw and separated liquid slurry. Parasitology, 140(3), 378-384. https://doi.org/10.1017/S0031182012001722

Vancouver

Katakam KK, Roepstorff AK, Popovic O, Kyvsgaard NC, Thamsborg SM, Dalsgaard A. Viability of Ascaris suum eggs in stored raw and separated liquid slurry. Parasitology. 2013;140(3):378-384. https://doi.org/10.1017/S0031182012001722

Author

Katakam, Kiran Kumar ; Roepstorff, Allan Knud ; Popovic, Olga ; Kyvsgaard, Niels Christian ; Thamsborg, Stig Milan ; Dalsgaard, Anders. / Viability of Ascaris suum eggs in stored raw and separated liquid slurry. In: Parasitology. 2013 ; Vol. 140, No. 3. pp. 378-384.

Bibtex

@article{5d31e72985144f319b3fe466dc5bce5a,
title = "Viability of Ascaris suum eggs in stored raw and separated liquid slurry",
abstract = "SUMMARY Separation of pig slurry into solid and liquid fractions is gaining importance as a way to manage increasing volumes of slurry. In contrast to solid manure and slurry, little is known about pathogen survival in separated liquid slurry. The viability of Ascaris suum eggs, a conservative indicator of fecal pollution, and its association with ammonia was investigated in separated liquid slurry in comparison with raw slurry. For this purpose nylon bags with 6000 eggs each were placed in 1 litre bottles containing one of the two fractions for 308 days at 5 °C or 25 °C. Initial analysis of helminth eggs in the separated liquid slurry revealed 47 Ascaris eggs per gramme. At 25 °C, egg viability declined to zero with a similar trend in both raw slurry and the separated liquid slurry by day 308, a time when at 5 °C 88{\%} and 42{\%} of the eggs were still viable in separated liquid slurry and raw slurry, respectively. The poorer survival at 25 °C was correlated with high ammonia contents in the range of 7·9-22·4 mm in raw slurry and 7·3-23·2 mm in liquid slurry compared to 3·2-9·5 mm in raw slurry and 2·6-9·5 mm in liquid slurry stored at 5 °C. The study demonstrates that at 5 °C, A. suum eggs have a higher viability in separated liquid slurry as compared to raw slurry. The hygiene aspect of this needs to be further investigated when separated liquid slurry is used to fertilize pastures or crops.",
keywords = "Former LIFE faculty, raw slurry, separated liquid slurry, viability, Ascaris suum eggs, temperature, ammonia",
author = "Katakam, {Kiran Kumar} and Roepstorff, {Allan Knud} and Olga Popovic and Kyvsgaard, {Niels Christian} and Thamsborg, {Stig Milan} and Anders Dalsgaard",
year = "2013",
doi = "10.1017/S0031182012001722",
language = "English",
volume = "140",
pages = "378--384",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Viability of Ascaris suum eggs in stored raw and separated liquid slurry

AU - Katakam, Kiran Kumar

AU - Roepstorff, Allan Knud

AU - Popovic, Olga

AU - Kyvsgaard, Niels Christian

AU - Thamsborg, Stig Milan

AU - Dalsgaard, Anders

PY - 2013

Y1 - 2013

N2 - SUMMARY Separation of pig slurry into solid and liquid fractions is gaining importance as a way to manage increasing volumes of slurry. In contrast to solid manure and slurry, little is known about pathogen survival in separated liquid slurry. The viability of Ascaris suum eggs, a conservative indicator of fecal pollution, and its association with ammonia was investigated in separated liquid slurry in comparison with raw slurry. For this purpose nylon bags with 6000 eggs each were placed in 1 litre bottles containing one of the two fractions for 308 days at 5 °C or 25 °C. Initial analysis of helminth eggs in the separated liquid slurry revealed 47 Ascaris eggs per gramme. At 25 °C, egg viability declined to zero with a similar trend in both raw slurry and the separated liquid slurry by day 308, a time when at 5 °C 88% and 42% of the eggs were still viable in separated liquid slurry and raw slurry, respectively. The poorer survival at 25 °C was correlated with high ammonia contents in the range of 7·9-22·4 mm in raw slurry and 7·3-23·2 mm in liquid slurry compared to 3·2-9·5 mm in raw slurry and 2·6-9·5 mm in liquid slurry stored at 5 °C. The study demonstrates that at 5 °C, A. suum eggs have a higher viability in separated liquid slurry as compared to raw slurry. The hygiene aspect of this needs to be further investigated when separated liquid slurry is used to fertilize pastures or crops.

AB - SUMMARY Separation of pig slurry into solid and liquid fractions is gaining importance as a way to manage increasing volumes of slurry. In contrast to solid manure and slurry, little is known about pathogen survival in separated liquid slurry. The viability of Ascaris suum eggs, a conservative indicator of fecal pollution, and its association with ammonia was investigated in separated liquid slurry in comparison with raw slurry. For this purpose nylon bags with 6000 eggs each were placed in 1 litre bottles containing one of the two fractions for 308 days at 5 °C or 25 °C. Initial analysis of helminth eggs in the separated liquid slurry revealed 47 Ascaris eggs per gramme. At 25 °C, egg viability declined to zero with a similar trend in both raw slurry and the separated liquid slurry by day 308, a time when at 5 °C 88% and 42% of the eggs were still viable in separated liquid slurry and raw slurry, respectively. The poorer survival at 25 °C was correlated with high ammonia contents in the range of 7·9-22·4 mm in raw slurry and 7·3-23·2 mm in liquid slurry compared to 3·2-9·5 mm in raw slurry and 2·6-9·5 mm in liquid slurry stored at 5 °C. The study demonstrates that at 5 °C, A. suum eggs have a higher viability in separated liquid slurry as compared to raw slurry. The hygiene aspect of this needs to be further investigated when separated liquid slurry is used to fertilize pastures or crops.

KW - Former LIFE faculty

KW - raw slurry

KW - separated liquid slurry

KW - viability

KW - Ascaris suum eggs

KW - temperature

KW - ammonia

U2 - 10.1017/S0031182012001722

DO - 10.1017/S0031182012001722

M3 - Journal article

C2 - 23127297

VL - 140

SP - 378

EP - 384

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 3

ER -

ID: 41860778