Tonoplast aquaporins facilitate lateral root emergence
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Tonoplast aquaporins facilitate lateral root emergence. / Reinhardt, Hagen; Hachez, Charles; Bienert, Manuela Désirée; Beebo, Azeez; Swarup, Kamal; Voß, Ute; Bouhidel, Karim; Frigerio, Lorenzo; Schjørring, Jan Kofod; Bennett, Malcolm J; Chaumont, Francois.
In: Plant Physiology, Vol. 170, No. 3, 2016, p. 1640-1654.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Tonoplast aquaporins facilitate lateral root emergence
AU - Reinhardt, Hagen
AU - Hachez, Charles
AU - Bienert, Manuela Désirée
AU - Beebo, Azeez
AU - Swarup, Kamal
AU - Voß, Ute
AU - Bouhidel, Karim
AU - Frigerio, Lorenzo
AU - Schjørring, Jan Kofod
AU - Bennett, Malcolm J
AU - Chaumont, Francois
N1 - © 2016 American Society of Plant Biologists. All Rights Reserved.
PY - 2016
Y1 - 2016
N2 - Aquaporins (AQPs) are water channels allowing fast and passive diffusion of water across cell membranes. It was hypothesized that AQPs contribute to cell elongation processes by allowing water influx across the plasma membrane and the tonoplast to maintain adequate turgor pressure. Here, we report that, in Arabidopsis (Arabidopsis thaliana), the highly abundant tonoplast AQP isoforms AtTIP1;1, AtTIP1;2, and AtTIP2;1 facilitate the emergence of new lateral root primordia (LRPs). The number of lateral roots was strongly reduced in the triple tip mutant, whereas the single, double, and triple tip mutants showed no or minor reduction in growth of the main root. This phenotype was due to the retardation of LRP emergence. Live cell imaging revealed that tight spatiotemporal control of TIP abundance in the tonoplast of the different LRP cells is pivotal to mediating this developmental process. While lateral root emergence is correlated to a reduction of AtTIP1;1 and AtTIP1;2 protein levels in LRPs, expression of AtTIP2;1 is specifically needed in a restricted cell population at the base, then later at the flanks, of developing LRPs. Interestingly, the LRP emergence phenotype of the triple tip mutants could be fully rescued by expressing AtTIP2;1 under its native promoter. We conclude that TIP isoforms allow the spatial and temporal fine-tuning of cellular water transport, which is critically required during the highly regulated process of LRP morphogenesis and emergence.
AB - Aquaporins (AQPs) are water channels allowing fast and passive diffusion of water across cell membranes. It was hypothesized that AQPs contribute to cell elongation processes by allowing water influx across the plasma membrane and the tonoplast to maintain adequate turgor pressure. Here, we report that, in Arabidopsis (Arabidopsis thaliana), the highly abundant tonoplast AQP isoforms AtTIP1;1, AtTIP1;2, and AtTIP2;1 facilitate the emergence of new lateral root primordia (LRPs). The number of lateral roots was strongly reduced in the triple tip mutant, whereas the single, double, and triple tip mutants showed no or minor reduction in growth of the main root. This phenotype was due to the retardation of LRP emergence. Live cell imaging revealed that tight spatiotemporal control of TIP abundance in the tonoplast of the different LRP cells is pivotal to mediating this developmental process. While lateral root emergence is correlated to a reduction of AtTIP1;1 and AtTIP1;2 protein levels in LRPs, expression of AtTIP2;1 is specifically needed in a restricted cell population at the base, then later at the flanks, of developing LRPs. Interestingly, the LRP emergence phenotype of the triple tip mutants could be fully rescued by expressing AtTIP2;1 under its native promoter. We conclude that TIP isoforms allow the spatial and temporal fine-tuning of cellular water transport, which is critically required during the highly regulated process of LRP morphogenesis and emergence.
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1104/pp.15.01635
DO - 10.1104/pp.15.01635
M3 - Journal article
C2 - 26802038
VL - 170
SP - 1640
EP - 1654
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 3
ER -
ID: 169136880