The CLE53-SUNN genetic pathway negatively regulates arbuscular mycorrhiza root colonization in Medicago truncatula

Research output: Contribution to journalJournal articlepeer-review

  • Magda Karlo
  • Clarissa Boschiero
  • Katrine Gram Landerslev
  • Gonzalo Sancho Blanco
  • Jiangqi Wen
  • Kirankumar S. Mysore
  • Xinbin Dai
  • Patrick X. Zhao
  • Thomas C. de Bang

Plants and arbuscular mycorrhizal fungi (AMF) engage in mutually beneficial symbioses based on a reciprocal exchange of nutrients. The beneficial character of the symbiosis is maintained through a mechanism called autoregulation of mycorrhization (AOM). AOM includes root-to-shoot-to-root signaling; however, the molecular details of AOM are poorly understood. AOM shares many features of autoregulation of nodulation (AON) where several genes are known, including the receptor-like kinase SUPER NUMERIC NODULES (SUNN), root-to-shoot mobile CLAVATA3/ENDOSPERM SURROUNDING REGION (ESR)-RELATED (CLE) peptides, and the hydroxyproline O-arabinosyltransferase ROOT DETERMINED NODULATION1 (RDN1) required for post-translational peptide modification. In this work, CLE53 was identified to negatively regulate AMF symbiosis in a SUNN- and RDN1-dependent manner. CLE53 expression was repressed at low phosphorus, while it was induced by AMF colonization and high phosphorus. CLE53 overexpression reduced AMF colonization in a SUNN- and RDN1 dependent manner, while cle53, rdn1, and sunn mutants were more colonized than the wild type. RNA-sequencing identified 700 genes with SUNN-dependent regulation in AMFcolonized plants, providing a resource for future identification of additional AOM genes. Disruption of AOM genes in crops potentially constitutes a novel route for improving AMF-derived phosphorus uptake in agricultural systems with high phosphorus levels.

Original languageEnglish
JournalJournal of Experimental Botany
Volume71
Issue number16
Pages (from-to)4972-4984
Number of pages13
ISSN0022-0957
DOIs
Publication statusPublished - 2020

    Research areas

  • Autoregulation, CLE peptides, Medicago truncatula, mycorrhiza, RDN1, SUNN, NODULE NUMBER, SIGNALING PATHWAYS, PEPTIDE SIGNALS, RECEPTOR KINASE, CLE PEPTIDES, SUNN GENE, NODULATION, AUTOREGULATION, ARABINOSYLATION, SUPPRESSION

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