TY - JOUR

T1 - The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity

AU - Schultz-Larsen, Torsten

AU - Lenk, Andrea

AU - Kalinowska, Kamila

AU - Vestergaard, Lau Kræsing

AU - Pedersen, Carsten

AU - Isono, Erika

AU - Thordal-Christensen, Hans

PY - 2018

Y1 - 2018

N2 - Plant “nucleotide-binding leucine-rich repeat” receptor proteins (NLRs) detect alterations in host targets of pathogen effectors and trigger immune responses. The Arabidopsis thaliana mutant pen1 syp122 displays autoimmunity, and a mutant screen identified the deubiquitinase “associated molecule with the SH3 domain of STAM3” (AMSH3) to be required for this phenotype. AMSH3 has previously been implicated in ESCRT-mediated vacuolar targeting. Pathology experiments show that AMSH3 activity is required for immunity mediated by the CC-NLRs, RPS2 and RPM1. Co-expressing the autoactive RPM1 D505V and the catalytically inactive ESCRT-III protein SKD1 E232Q in Nicotiana benthamiana supports the requirement of ESCRT-associated functions for this CC-NLR-activated immunity. Meanwhile, loss of ESCRT function in A. thaliana is lethal, and we find that AMSH3 knockout-triggered seedling lethality is “enhanced disease susceptibility 1” (EDS1) dependent. Future studies may reveal whether AMSH3 is monitored by a TIR-NLR immunity receptor. NLR receptors are important for plant immunity as they monitor plant processes that are targeted by pathogens. Schultz-Larsen et al. find that the AMSH3 deubiquitinase enzyme is important for immunity activated by specific NLRs. Furthermore, their results suggest that AMSH3 itself is monitored by other NLRs.

AB - Plant “nucleotide-binding leucine-rich repeat” receptor proteins (NLRs) detect alterations in host targets of pathogen effectors and trigger immune responses. The Arabidopsis thaliana mutant pen1 syp122 displays autoimmunity, and a mutant screen identified the deubiquitinase “associated molecule with the SH3 domain of STAM3” (AMSH3) to be required for this phenotype. AMSH3 has previously been implicated in ESCRT-mediated vacuolar targeting. Pathology experiments show that AMSH3 activity is required for immunity mediated by the CC-NLRs, RPS2 and RPM1. Co-expressing the autoactive RPM1 D505V and the catalytically inactive ESCRT-III protein SKD1 E232Q in Nicotiana benthamiana supports the requirement of ESCRT-associated functions for this CC-NLR-activated immunity. Meanwhile, loss of ESCRT function in A. thaliana is lethal, and we find that AMSH3 knockout-triggered seedling lethality is “enhanced disease susceptibility 1” (EDS1) dependent. Future studies may reveal whether AMSH3 is monitored by a TIR-NLR immunity receptor. NLR receptors are important for plant immunity as they monitor plant processes that are targeted by pathogens. Schultz-Larsen et al. find that the AMSH3 deubiquitinase enzyme is important for immunity activated by specific NLRs. Furthermore, their results suggest that AMSH3 itself is monitored by other NLRs.

KW - AMSH3 deubiquitinase

KW - ESCRT

KW - autoimmunity

KW - nucleotide-binding leucine-rich repeat receptors

KW - plant immunity

U2 - 10.1016/j.celrep.2018.11.011

DO - 10.1016/j.celrep.2018.11.011

M3 - Journal article

C2 - 30485803

VL - 25

SP - 2329-2338.e5

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 9

ER -