The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity

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The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity. / Schultz-Larsen, Torsten; Lenk, Andrea; Kalinowska, Kamila; Vestergaard, Lau Kræsing; Pedersen, Carsten; Isono, Erika; Thordal-Christensen, Hans.

In: Cell Reports, Vol. 25, No. 9, 2018, p. 2329-2338.e5.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Schultz-Larsen, T, Lenk, A, Kalinowska, K, Vestergaard, LK, Pedersen, C, Isono, E & Thordal-Christensen, H 2018, 'The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity', Cell Reports, vol. 25, no. 9, pp. 2329-2338.e5. https://doi.org/10.1016/j.celrep.2018.11.011

APA

Schultz-Larsen, T., Lenk, A., Kalinowska, K., Vestergaard, L. K., Pedersen, C., Isono, E., & Thordal-Christensen, H. (2018). The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity. Cell Reports, 25(9), 2329-2338.e5. https://doi.org/10.1016/j.celrep.2018.11.011

Vancouver

Schultz-Larsen T, Lenk A, Kalinowska K, Vestergaard LK, Pedersen C, Isono E et al. The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity. Cell Reports. 2018;25(9):2329-2338.e5. https://doi.org/10.1016/j.celrep.2018.11.011

Author

Schultz-Larsen, Torsten ; Lenk, Andrea ; Kalinowska, Kamila ; Vestergaard, Lau Kræsing ; Pedersen, Carsten ; Isono, Erika ; Thordal-Christensen, Hans. / The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity. In: Cell Reports. 2018 ; Vol. 25, No. 9. pp. 2329-2338.e5.

Bibtex

@article{009a22dfa6ca4a88a60efe3ed4ab5810,
title = "The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity",
abstract = "Plant “nucleotide-binding leucine-rich repeat” receptor proteins (NLRs) detect alterations in host targets of pathogen effectors and trigger immune responses. The Arabidopsis thaliana mutant pen1 syp122 displays autoimmunity, and a mutant screen identified the deubiquitinase “associated molecule with the SH3 domain of STAM3” (AMSH3) to be required for this phenotype. AMSH3 has previously been implicated in ESCRT-mediated vacuolar targeting. Pathology experiments show that AMSH3 activity is required for immunity mediated by the CC-NLRs, RPS2 and RPM1. Co-expressing the autoactive RPM1 D505V and the catalytically inactive ESCRT-III protein SKD1 E232Q in Nicotiana benthamiana supports the requirement of ESCRT-associated functions for this CC-NLR-activated immunity. Meanwhile, loss of ESCRT function in A. thaliana is lethal, and we find that AMSH3 knockout-triggered seedling lethality is “enhanced disease susceptibility 1” (EDS1) dependent. Future studies may reveal whether AMSH3 is monitored by a TIR-NLR immunity receptor. NLR receptors are important for plant immunity as they monitor plant processes that are targeted by pathogens. Schultz-Larsen et al. find that the AMSH3 deubiquitinase enzyme is important for immunity activated by specific NLRs. Furthermore, their results suggest that AMSH3 itself is monitored by other NLRs.",
keywords = "AMSH3 deubiquitinase, ESCRT, autoimmunity, nucleotide-binding leucine-rich repeat receptors, plant immunity",
author = "Torsten Schultz-Larsen and Andrea Lenk and Kamila Kalinowska and Vestergaard, {Lau Kr{\ae}sing} and Carsten Pedersen and Erika Isono and Hans Thordal-Christensen",
year = "2018",
doi = "10.1016/j.celrep.2018.11.011",
language = "English",
volume = "25",
pages = "2329--2338.e5",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "9",

}

RIS

TY - JOUR

T1 - The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity

AU - Schultz-Larsen, Torsten

AU - Lenk, Andrea

AU - Kalinowska, Kamila

AU - Vestergaard, Lau Kræsing

AU - Pedersen, Carsten

AU - Isono, Erika

AU - Thordal-Christensen, Hans

PY - 2018

Y1 - 2018

N2 - Plant “nucleotide-binding leucine-rich repeat” receptor proteins (NLRs) detect alterations in host targets of pathogen effectors and trigger immune responses. The Arabidopsis thaliana mutant pen1 syp122 displays autoimmunity, and a mutant screen identified the deubiquitinase “associated molecule with the SH3 domain of STAM3” (AMSH3) to be required for this phenotype. AMSH3 has previously been implicated in ESCRT-mediated vacuolar targeting. Pathology experiments show that AMSH3 activity is required for immunity mediated by the CC-NLRs, RPS2 and RPM1. Co-expressing the autoactive RPM1 D505V and the catalytically inactive ESCRT-III protein SKD1 E232Q in Nicotiana benthamiana supports the requirement of ESCRT-associated functions for this CC-NLR-activated immunity. Meanwhile, loss of ESCRT function in A. thaliana is lethal, and we find that AMSH3 knockout-triggered seedling lethality is “enhanced disease susceptibility 1” (EDS1) dependent. Future studies may reveal whether AMSH3 is monitored by a TIR-NLR immunity receptor. NLR receptors are important for plant immunity as they monitor plant processes that are targeted by pathogens. Schultz-Larsen et al. find that the AMSH3 deubiquitinase enzyme is important for immunity activated by specific NLRs. Furthermore, their results suggest that AMSH3 itself is monitored by other NLRs.

AB - Plant “nucleotide-binding leucine-rich repeat” receptor proteins (NLRs) detect alterations in host targets of pathogen effectors and trigger immune responses. The Arabidopsis thaliana mutant pen1 syp122 displays autoimmunity, and a mutant screen identified the deubiquitinase “associated molecule with the SH3 domain of STAM3” (AMSH3) to be required for this phenotype. AMSH3 has previously been implicated in ESCRT-mediated vacuolar targeting. Pathology experiments show that AMSH3 activity is required for immunity mediated by the CC-NLRs, RPS2 and RPM1. Co-expressing the autoactive RPM1 D505V and the catalytically inactive ESCRT-III protein SKD1 E232Q in Nicotiana benthamiana supports the requirement of ESCRT-associated functions for this CC-NLR-activated immunity. Meanwhile, loss of ESCRT function in A. thaliana is lethal, and we find that AMSH3 knockout-triggered seedling lethality is “enhanced disease susceptibility 1” (EDS1) dependent. Future studies may reveal whether AMSH3 is monitored by a TIR-NLR immunity receptor. NLR receptors are important for plant immunity as they monitor plant processes that are targeted by pathogens. Schultz-Larsen et al. find that the AMSH3 deubiquitinase enzyme is important for immunity activated by specific NLRs. Furthermore, their results suggest that AMSH3 itself is monitored by other NLRs.

KW - AMSH3 deubiquitinase

KW - ESCRT

KW - autoimmunity

KW - nucleotide-binding leucine-rich repeat receptors

KW - plant immunity

U2 - 10.1016/j.celrep.2018.11.011

DO - 10.1016/j.celrep.2018.11.011

M3 - Journal article

C2 - 30485803

VL - 25

SP - 2329-2338.e5

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 9

ER -

ID: 209806323