Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A

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Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A. / Theilade, Bodil; Rasmussen, Søren K.

In: Gene, Vol. 118, No. 2, 10.09.1992, p. 261-266.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Theilade, B & Rasmussen, SK 1992, 'Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A', Gene, vol. 118, no. 2, pp. 261-266. https://doi.org/10.1016/0378-1119(92)90197-W

APA

Theilade, B., & Rasmussen, S. K. (1992). Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A. Gene, 118(2), 261-266. https://doi.org/10.1016/0378-1119(92)90197-W

Vancouver

Theilade B, Rasmussen SK. Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A. Gene. 1992 Sep 10;118(2):261-266. https://doi.org/10.1016/0378-1119(92)90197-W

Author

Theilade, Bodil ; Rasmussen, Søren K. / Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A. In: Gene. 1992 ; Vol. 118, No. 2. pp. 261-266.

Bibtex

@article{0be0cb806a754de1a9d156d67e01e6b8,
title = "Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A",
abstract = "A clone, λPrx6.1, coding for a barley seed peroxidase (BP; EC 1.11.1.7), was isolated from a genomic library using a cDNA coding for the barley seed peroxidase, BP 1, as a probe. The nucleotide sequence coded for a BP showing 73% amino acid (aa) sequence identity with BP l and less than 50% similarity with other sequenced plant peroxidases. The aa composition is 92% identical to that determined for BP 2 purified from mature barley grains, and therefore the gene product is named BP 2A. The alignment suggests that the coding region is interrupted by a 76-bp intron having the consensuses GT and AG, at the 5' and 3' ends, respectively. Alignment with BP 1 suggests that BP 2A has a leader peptide of 36 aa and the mature protein is 319 aa. Alanine and leucine account for 50% of the residues of the leader peptide. Of the codons used 90% have a C or G in the third position. The promoter shows a putative abscisic acid-response element, 5'-GTA-CGTGTC, 115 bp upstream from the start codon. The BP 2A-encoding gene was RFLP-mapped on barley chromosome 3, and we suggest for this peroxidase locus the name Prx6.",
keywords = "abscisic acid, C-terminal processing, Hordeum vulgare, intron, nucleotide sequence, RFLP, signal peptide",
author = "Bodil Theilade and Rasmussen, {S{\o}ren K.}",
year = "1992",
month = sep,
day = "10",
doi = "10.1016/0378-1119(92)90197-W",
language = "English",
volume = "118",
pages = "261--266",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A

AU - Theilade, Bodil

AU - Rasmussen, Søren K.

PY - 1992/9/10

Y1 - 1992/9/10

N2 - A clone, λPrx6.1, coding for a barley seed peroxidase (BP; EC 1.11.1.7), was isolated from a genomic library using a cDNA coding for the barley seed peroxidase, BP 1, as a probe. The nucleotide sequence coded for a BP showing 73% amino acid (aa) sequence identity with BP l and less than 50% similarity with other sequenced plant peroxidases. The aa composition is 92% identical to that determined for BP 2 purified from mature barley grains, and therefore the gene product is named BP 2A. The alignment suggests that the coding region is interrupted by a 76-bp intron having the consensuses GT and AG, at the 5' and 3' ends, respectively. Alignment with BP 1 suggests that BP 2A has a leader peptide of 36 aa and the mature protein is 319 aa. Alanine and leucine account for 50% of the residues of the leader peptide. Of the codons used 90% have a C or G in the third position. The promoter shows a putative abscisic acid-response element, 5'-GTA-CGTGTC, 115 bp upstream from the start codon. The BP 2A-encoding gene was RFLP-mapped on barley chromosome 3, and we suggest for this peroxidase locus the name Prx6.

AB - A clone, λPrx6.1, coding for a barley seed peroxidase (BP; EC 1.11.1.7), was isolated from a genomic library using a cDNA coding for the barley seed peroxidase, BP 1, as a probe. The nucleotide sequence coded for a BP showing 73% amino acid (aa) sequence identity with BP l and less than 50% similarity with other sequenced plant peroxidases. The aa composition is 92% identical to that determined for BP 2 purified from mature barley grains, and therefore the gene product is named BP 2A. The alignment suggests that the coding region is interrupted by a 76-bp intron having the consensuses GT and AG, at the 5' and 3' ends, respectively. Alignment with BP 1 suggests that BP 2A has a leader peptide of 36 aa and the mature protein is 319 aa. Alanine and leucine account for 50% of the residues of the leader peptide. Of the codons used 90% have a C or G in the third position. The promoter shows a putative abscisic acid-response element, 5'-GTA-CGTGTC, 115 bp upstream from the start codon. The BP 2A-encoding gene was RFLP-mapped on barley chromosome 3, and we suggest for this peroxidase locus the name Prx6.

KW - abscisic acid

KW - C-terminal processing

KW - Hordeum vulgare

KW - intron

KW - nucleotide sequence

KW - RFLP

KW - signal peptide

UR - http://www.scopus.com/inward/record.url?scp=0026698748&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(92)90197-W

DO - 10.1016/0378-1119(92)90197-W

M3 - Journal article

C2 - 1355062

AN - SCOPUS:0026698748

VL - 118

SP - 261

EP - 266

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -

ID: 204471104