Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A
Research output: Contribution to journal › Journal article › peer-review
Standard
Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A. / Theilade, Bodil; Rasmussen, Søren K.
In: Gene, Vol. 118, No. 2, 10.09.1992, p. 261-266.Research output: Contribution to journal › Journal article › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A
AU - Theilade, Bodil
AU - Rasmussen, Søren K.
PY - 1992/9/10
Y1 - 1992/9/10
N2 - A clone, λPrx6.1, coding for a barley seed peroxidase (BP; EC 1.11.1.7), was isolated from a genomic library using a cDNA coding for the barley seed peroxidase, BP 1, as a probe. The nucleotide sequence coded for a BP showing 73% amino acid (aa) sequence identity with BP l and less than 50% similarity with other sequenced plant peroxidases. The aa composition is 92% identical to that determined for BP 2 purified from mature barley grains, and therefore the gene product is named BP 2A. The alignment suggests that the coding region is interrupted by a 76-bp intron having the consensuses GT and AG, at the 5' and 3' ends, respectively. Alignment with BP 1 suggests that BP 2A has a leader peptide of 36 aa and the mature protein is 319 aa. Alanine and leucine account for 50% of the residues of the leader peptide. Of the codons used 90% have a C or G in the third position. The promoter shows a putative abscisic acid-response element, 5'-GTA-CGTGTC, 115 bp upstream from the start codon. The BP 2A-encoding gene was RFLP-mapped on barley chromosome 3, and we suggest for this peroxidase locus the name Prx6.
AB - A clone, λPrx6.1, coding for a barley seed peroxidase (BP; EC 1.11.1.7), was isolated from a genomic library using a cDNA coding for the barley seed peroxidase, BP 1, as a probe. The nucleotide sequence coded for a BP showing 73% amino acid (aa) sequence identity with BP l and less than 50% similarity with other sequenced plant peroxidases. The aa composition is 92% identical to that determined for BP 2 purified from mature barley grains, and therefore the gene product is named BP 2A. The alignment suggests that the coding region is interrupted by a 76-bp intron having the consensuses GT and AG, at the 5' and 3' ends, respectively. Alignment with BP 1 suggests that BP 2A has a leader peptide of 36 aa and the mature protein is 319 aa. Alanine and leucine account for 50% of the residues of the leader peptide. Of the codons used 90% have a C or G in the third position. The promoter shows a putative abscisic acid-response element, 5'-GTA-CGTGTC, 115 bp upstream from the start codon. The BP 2A-encoding gene was RFLP-mapped on barley chromosome 3, and we suggest for this peroxidase locus the name Prx6.
KW - abscisic acid
KW - C-terminal processing
KW - Hordeum vulgare
KW - intron
KW - nucleotide sequence
KW - RFLP
KW - signal peptide
UR - http://www.scopus.com/inward/record.url?scp=0026698748&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(92)90197-W
DO - 10.1016/0378-1119(92)90197-W
M3 - Journal article
C2 - 1355062
AN - SCOPUS:0026698748
VL - 118
SP - 261
EP - 266
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -
ID: 204471104