Rice F-bZIP transcription factors regulate the zinc deficiency response
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Rice F-bZIP transcription factors regulate the zinc deficiency response. / Lilay, Grmay H.; Castro, Pedro Humberto; Guedes, Joana G; Almeida, Diego M; Campilho, Ana; Azevedo, Herlander; Aarts, Mark G M; Saibo, Nelson J.M.; Assunção, Ana G.L.
In: Journal of Experimental Botany, Vol. 71, No. 12, 2020, p. 3664-3677.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Rice F-bZIP transcription factors regulate the zinc deficiency response
AU - Lilay, Grmay H.
AU - Castro, Pedro Humberto
AU - Guedes, Joana G
AU - Almeida, Diego M
AU - Campilho, Ana
AU - Azevedo, Herlander
AU - Aarts, Mark G M
AU - Saibo, Nelson J.M.
AU - Assunção, Ana G.L.
PY - 2020
Y1 - 2020
N2 - F-bZIP transcription factors bZIP19 and bZIP23 are the central regulators of the zinc deficiency response in Arabidopsis, and phylogenetic analysis of F-bZIP homologs across land plants indicates conservation of the zinc deficiency response regulatory mechanism. Here, we identified the rice F-bZIP homologs and investigated their function. OsbZIP48 and OsbZIP50, but not OsbZIP49, complement the zinc deficiency hypersensitive Arabidopsis bzip19bzip23 double mutant. Ectopic expression of OsbZIP50 in Arabidopsis significantly increases plant zinc accumulation under control zinc supply, suggesting an altered Zn sensing in OsbZIP50. In addition, we performed a phylogenetic analysis of F-bZIP homologs from representative Monocot species that supports the branching of plant F-bZIPs into Group 1 and Group 2. Our results suggest conservation of the zinc deficiency response regulation in rice, with OsbZIP48 being a functional homolog of AtbZIP19 and AtbZIP23. A better understanding of the mechanisms behind the Zn deficiency response in rice and other important crops will contribute to develop plant-based strategies to address the problems of Zn deficiency in soils, crops and cereal-based human diets.
AB - F-bZIP transcription factors bZIP19 and bZIP23 are the central regulators of the zinc deficiency response in Arabidopsis, and phylogenetic analysis of F-bZIP homologs across land plants indicates conservation of the zinc deficiency response regulatory mechanism. Here, we identified the rice F-bZIP homologs and investigated their function. OsbZIP48 and OsbZIP50, but not OsbZIP49, complement the zinc deficiency hypersensitive Arabidopsis bzip19bzip23 double mutant. Ectopic expression of OsbZIP50 in Arabidopsis significantly increases plant zinc accumulation under control zinc supply, suggesting an altered Zn sensing in OsbZIP50. In addition, we performed a phylogenetic analysis of F-bZIP homologs from representative Monocot species that supports the branching of plant F-bZIPs into Group 1 and Group 2. Our results suggest conservation of the zinc deficiency response regulation in rice, with OsbZIP48 being a functional homolog of AtbZIP19 and AtbZIP23. A better understanding of the mechanisms behind the Zn deficiency response in rice and other important crops will contribute to develop plant-based strategies to address the problems of Zn deficiency in soils, crops and cereal-based human diets.
U2 - 10.1093/jxb/eraa115
DO - 10.1093/jxb/eraa115
M3 - Journal article
C2 - 32133499
VL - 71
SP - 3664
EP - 3677
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
SN - 0022-0957
IS - 12
ER -
ID: 237371508