Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection. / Møller, Laura Hyrup; Macherius, André; Hansen, Thomas Hesselhøj; Nielsen, Hanne Mørck; Cornett, Claus; Østergaard, Jesper; Stürup, Stefan; Gammelgaard, Bente.

In: Journal of Analytical Atomic Spectrometry, Vol. 31, 2016, p. 1877-1884.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Møller, LH, Macherius, A, Hansen, TH, Nielsen, HM, Cornett, C, Østergaard, J, Stürup, S & Gammelgaard, B 2016, 'Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection', Journal of Analytical Atomic Spectrometry, vol. 31, pp. 1877-1884. https://doi.org/10.1039/C6JA00132G

APA

Møller, L. H., Macherius, A., Hansen, T. H., Nielsen, H. M., Cornett, C., Østergaard, J., ... Gammelgaard, B. (2016). Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection. Journal of Analytical Atomic Spectrometry, 31, 1877-1884. https://doi.org/10.1039/C6JA00132G

Vancouver

Møller LH, Macherius A, Hansen TH, Nielsen HM, Cornett C, Østergaard J et al. Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection. Journal of Analytical Atomic Spectrometry. 2016;31:1877-1884. https://doi.org/10.1039/C6JA00132G

Author

Møller, Laura Hyrup ; Macherius, André ; Hansen, Thomas Hesselhøj ; Nielsen, Hanne Mørck ; Cornett, Claus ; Østergaard, Jesper ; Stürup, Stefan ; Gammelgaard, Bente. / Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection. In: Journal of Analytical Atomic Spectrometry. 2016 ; Vol. 31. pp. 1877-1884.

Bibtex

@article{364a8cab77814f489eb9f875ce7d9f2e,
title = "Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection",
abstract = "A method for quantification of a pharmaceutical peptide in human plasma was developed using gradient elution LC-ICP-MS. A membrane desolvation (MD) system was applied to remove organic solvents from the eluent prior to the detection as SO+ in the dynamic reaction cell (DRC) of the ICP-DRC-MS instrument and subsequent quantification by post-column isotope dilution (IDA). Plasma proteins were precipitated prior to analysis. Analytical figures of merit including linearity, precision, LOD, LOQ and accuracy were considered satisfactory for analysis of plasma samples. The selectivity of the developed method was demonstrated for five pharmaceutically relevant peptides: desmopressin, penetratin, substance P, PTH (1-34) and insulin. Preliminary experiments on an ICP-MS/MS system using oxygen to reduce the effect of organic solvents were also performed to compare sensitivity. The results of the study demonstrated that LC-ICP-MS post-column IDA may constitute a valuable additional tool in quantification of non-labelled peptides in the early drug development offering absolute quantification without need of species specific standards.",
author = "M{\o}ller, {Laura Hyrup} and Andr{\'e} Macherius and Hansen, {Thomas Hesselh{\o}j} and Nielsen, {Hanne M{\o}rck} and Claus Cornett and Jesper {\O}stergaard and Stefan St{\"u}rup and Bente Gammelgaard",
year = "2016",
doi = "10.1039/C6JA00132G",
language = "English",
volume = "31",
pages = "1877--1884",
journal = "Journal of Analytical Atomic Spectrometry",
issn = "0267-9477",
publisher = "Royal Society of Chemistry",

}

RIS

TY - JOUR

T1 - Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection

AU - Møller, Laura Hyrup

AU - Macherius, André

AU - Hansen, Thomas Hesselhøj

AU - Nielsen, Hanne Mørck

AU - Cornett, Claus

AU - Østergaard, Jesper

AU - Stürup, Stefan

AU - Gammelgaard, Bente

PY - 2016

Y1 - 2016

N2 - A method for quantification of a pharmaceutical peptide in human plasma was developed using gradient elution LC-ICP-MS. A membrane desolvation (MD) system was applied to remove organic solvents from the eluent prior to the detection as SO+ in the dynamic reaction cell (DRC) of the ICP-DRC-MS instrument and subsequent quantification by post-column isotope dilution (IDA). Plasma proteins were precipitated prior to analysis. Analytical figures of merit including linearity, precision, LOD, LOQ and accuracy were considered satisfactory for analysis of plasma samples. The selectivity of the developed method was demonstrated for five pharmaceutically relevant peptides: desmopressin, penetratin, substance P, PTH (1-34) and insulin. Preliminary experiments on an ICP-MS/MS system using oxygen to reduce the effect of organic solvents were also performed to compare sensitivity. The results of the study demonstrated that LC-ICP-MS post-column IDA may constitute a valuable additional tool in quantification of non-labelled peptides in the early drug development offering absolute quantification without need of species specific standards.

AB - A method for quantification of a pharmaceutical peptide in human plasma was developed using gradient elution LC-ICP-MS. A membrane desolvation (MD) system was applied to remove organic solvents from the eluent prior to the detection as SO+ in the dynamic reaction cell (DRC) of the ICP-DRC-MS instrument and subsequent quantification by post-column isotope dilution (IDA). Plasma proteins were precipitated prior to analysis. Analytical figures of merit including linearity, precision, LOD, LOQ and accuracy were considered satisfactory for analysis of plasma samples. The selectivity of the developed method was demonstrated for five pharmaceutically relevant peptides: desmopressin, penetratin, substance P, PTH (1-34) and insulin. Preliminary experiments on an ICP-MS/MS system using oxygen to reduce the effect of organic solvents were also performed to compare sensitivity. The results of the study demonstrated that LC-ICP-MS post-column IDA may constitute a valuable additional tool in quantification of non-labelled peptides in the early drug development offering absolute quantification without need of species specific standards.

U2 - 10.1039/C6JA00132G

DO - 10.1039/C6JA00132G

M3 - Journal article

VL - 31

SP - 1877

EP - 1884

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

SN - 0267-9477

ER -

ID: 164887608