Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans

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Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans. / Peng, Chuantao; Andersen, Birgit; Arshid, Samina; Larsen, Martin R.; Albergaria, Helena; Lametsch, Rene; Arneborg, Nils.

In: FEMS Microbiology Ecology, Vol. 95, No. 9, fiz126, 01.09.2019, p. 1-16.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Peng, C, Andersen, B, Arshid, S, Larsen, MR, Albergaria, H, Lametsch, R & Arneborg, N 2019, 'Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans', FEMS Microbiology Ecology, vol. 95, no. 9, fiz126, pp. 1-16. https://doi.org/10.1093/femsec/fiz126

APA

Peng, C., Andersen, B., Arshid, S., Larsen, M. R., Albergaria, H., Lametsch, R., & Arneborg, N. (2019). Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans. FEMS Microbiology Ecology, 95(9), 1-16. [fiz126]. https://doi.org/10.1093/femsec/fiz126

Vancouver

Peng C, Andersen B, Arshid S, Larsen MR, Albergaria H, Lametsch R et al. Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans. FEMS Microbiology Ecology. 2019 Sep 1;95(9):1-16. fiz126. https://doi.org/10.1093/femsec/fiz126

Author

Peng, Chuantao ; Andersen, Birgit ; Arshid, Samina ; Larsen, Martin R. ; Albergaria, Helena ; Lametsch, Rene ; Arneborg, Nils. / Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans. In: FEMS Microbiology Ecology. 2019 ; Vol. 95, No. 9. pp. 1-16.

Bibtex

@article{76e5dd9983f848caa8e6a9fe2147906c,
title = "Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans",
abstract = "The response of Saccharomyces cerevisiae to cocultivation with Lachancea thermotolerans during alcoholic fermentations has been investigated using tandem mass tag (TMT)-based proteomics. At two key time-points, S. cerevisiae was sorted from single S. cerevisiae fermentations and from mixed fermentations using flow cytometry sorting. Results showed that the purity of sorted S. cerevisiae was above 96{\%} throughout the whole mixed-culture fermentation, thereby validating our sorting methodology. By comparing protein expression of S. cerevisiae with and without L. thermotolerans, 26 proteins were identified as significantly regulated proteins at the early death phase (T1), and 32 significantly regulated proteins were identified at the late death phase (T2) of L. thermotolerans in mixed cultures. At T1, proteins involved in endocytosis, increasing nutrient availability, cell rescue and resistance to stresses were upregulated, and proteins involved in proline synthesis and apoptosis were downregulated. At T2, proteins involved in protein synthesis and stress responses were up- and downregulated, respectively. These data indicate that S. cerevisiae was stressed by the presence of L. thermotolerans at T1, using both defensive and fighting strategies to keep itself in a dominant position, and that it at T2 was relieved from stress, perhaps increasing its enzymatic machinery to ensure better survival.",
keywords = "alcoholic fermentations, cell sorting, flow cytometry, proteomic response, TMT-based proteomics, yeast-yeast interactions",
author = "Chuantao Peng and Birgit Andersen and Samina Arshid and Larsen, {Martin R.} and Helena Albergaria and Rene Lametsch and Nils Arneborg",
year = "2019",
month = "9",
day = "1",
doi = "10.1093/femsec/fiz126",
language = "English",
volume = "95",
pages = "1--16",
journal = "F E M S Microbiology Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "9",

}

RIS

TY - JOUR

T1 - Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans

AU - Peng, Chuantao

AU - Andersen, Birgit

AU - Arshid, Samina

AU - Larsen, Martin R.

AU - Albergaria, Helena

AU - Lametsch, Rene

AU - Arneborg, Nils

PY - 2019/9/1

Y1 - 2019/9/1

N2 - The response of Saccharomyces cerevisiae to cocultivation with Lachancea thermotolerans during alcoholic fermentations has been investigated using tandem mass tag (TMT)-based proteomics. At two key time-points, S. cerevisiae was sorted from single S. cerevisiae fermentations and from mixed fermentations using flow cytometry sorting. Results showed that the purity of sorted S. cerevisiae was above 96% throughout the whole mixed-culture fermentation, thereby validating our sorting methodology. By comparing protein expression of S. cerevisiae with and without L. thermotolerans, 26 proteins were identified as significantly regulated proteins at the early death phase (T1), and 32 significantly regulated proteins were identified at the late death phase (T2) of L. thermotolerans in mixed cultures. At T1, proteins involved in endocytosis, increasing nutrient availability, cell rescue and resistance to stresses were upregulated, and proteins involved in proline synthesis and apoptosis were downregulated. At T2, proteins involved in protein synthesis and stress responses were up- and downregulated, respectively. These data indicate that S. cerevisiae was stressed by the presence of L. thermotolerans at T1, using both defensive and fighting strategies to keep itself in a dominant position, and that it at T2 was relieved from stress, perhaps increasing its enzymatic machinery to ensure better survival.

AB - The response of Saccharomyces cerevisiae to cocultivation with Lachancea thermotolerans during alcoholic fermentations has been investigated using tandem mass tag (TMT)-based proteomics. At two key time-points, S. cerevisiae was sorted from single S. cerevisiae fermentations and from mixed fermentations using flow cytometry sorting. Results showed that the purity of sorted S. cerevisiae was above 96% throughout the whole mixed-culture fermentation, thereby validating our sorting methodology. By comparing protein expression of S. cerevisiae with and without L. thermotolerans, 26 proteins were identified as significantly regulated proteins at the early death phase (T1), and 32 significantly regulated proteins were identified at the late death phase (T2) of L. thermotolerans in mixed cultures. At T1, proteins involved in endocytosis, increasing nutrient availability, cell rescue and resistance to stresses were upregulated, and proteins involved in proline synthesis and apoptosis were downregulated. At T2, proteins involved in protein synthesis and stress responses were up- and downregulated, respectively. These data indicate that S. cerevisiae was stressed by the presence of L. thermotolerans at T1, using both defensive and fighting strategies to keep itself in a dominant position, and that it at T2 was relieved from stress, perhaps increasing its enzymatic machinery to ensure better survival.

KW - alcoholic fermentations

KW - cell sorting

KW - flow cytometry

KW - proteomic response

KW - TMT-based proteomics

KW - yeast-yeast interactions

UR - http://www.scopus.com/inward/record.url?scp=85071900039&partnerID=8YFLogxK

U2 - 10.1093/femsec/fiz126

DO - 10.1093/femsec/fiz126

M3 - Journal article

C2 - 31418786

AN - SCOPUS:85071900039

VL - 95

SP - 1

EP - 16

JO - F E M S Microbiology Ecology

JF - F E M S Microbiology Ecology

SN - 0168-6496

IS - 9

M1 - fiz126

ER -

ID: 227569103