Primary structure of A B1 hordein gene from barley
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Primary structure of A B1 hordein gene from barley. / Brandt, Anders; Montembault, Alain; Cameron-Mills, Verena; Rasmussen, Søren K.
In: Carlsberg Research Communications, Vol. 50, No. 6, 01.11.1985, p. 333-345.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Primary structure of A B1 hordein gene from barley
AU - Brandt, Anders
AU - Montembault, Alain
AU - Cameron-Mills, Verena
AU - Rasmussen, Søren K.
PY - 1985/11/1
Y1 - 1985/11/1
N2 - The 873 base pair coding region of a Hor-2 gene of barley and the adjacent 550 base pair upstream and 413 base pair downstream regions were sequenced. The gene is devoid of introns and encodes a 271 amino acid long B1 hordein polypeptide containing a putative 19 amino acid signal peptide. The remaining part of the coding sequence can be divided into three parts. In the 53 residue amino-terminal region there are 9 glutamine-proline blocks with a preferred core sequence PQQP, separated by one or two other residues giving a glutamine proline content of 78%. The second part encodes 164 amino acids, 41% of which are glutamine+proline organised in scattered blocks. Seven cysteine residues are coded for by this portion of the gene. The last part encodes the carboxyterminal 35 amino acids none of which is glutamine. In the 550 base pair upstream region the sequence TATAAATA is found at- 71 base pairs from the initiator methionine. In the 3′ non-coding region three putative polyadenylation signals, AATAAA, are present. Comparison of the gene with 3 partial cDNA clones indicates that the charge polymorphism in the B1 polypeptide group is due to point mutations in the part of the gene corresponding to the carboxy terminal half of the polypeptide. Comparison with the sequence of a second B hordein gene suggests that insertions or deletions of glutamine-proline blocks in the amino-terminal domain are a major source of size polymorphisms in the B hordein family.
AB - The 873 base pair coding region of a Hor-2 gene of barley and the adjacent 550 base pair upstream and 413 base pair downstream regions were sequenced. The gene is devoid of introns and encodes a 271 amino acid long B1 hordein polypeptide containing a putative 19 amino acid signal peptide. The remaining part of the coding sequence can be divided into three parts. In the 53 residue amino-terminal region there are 9 glutamine-proline blocks with a preferred core sequence PQQP, separated by one or two other residues giving a glutamine proline content of 78%. The second part encodes 164 amino acids, 41% of which are glutamine+proline organised in scattered blocks. Seven cysteine residues are coded for by this portion of the gene. The last part encodes the carboxyterminal 35 amino acids none of which is glutamine. In the 550 base pair upstream region the sequence TATAAATA is found at- 71 base pairs from the initiator methionine. In the 3′ non-coding region three putative polyadenylation signals, AATAAA, are present. Comparison of the gene with 3 partial cDNA clones indicates that the charge polymorphism in the B1 polypeptide group is due to point mutations in the part of the gene corresponding to the carboxy terminal half of the polypeptide. Comparison with the sequence of a second B hordein gene suggests that insertions or deletions of glutamine-proline blocks in the amino-terminal domain are a major source of size polymorphisms in the B hordein family.
KW - Endosperm
KW - gene family
KW - heterogeneity
KW - Hordeum vulgare
KW - molecular cloning
KW - storage protein
UR - http://www.scopus.com/inward/record.url?scp=0002889493&partnerID=8YFLogxK
U2 - 10.1007/BF02907156
DO - 10.1007/BF02907156
M3 - Journal article
AN - SCOPUS:0002889493
VL - 50
SP - 333
EP - 345
JO - Carlsberg Research Communications
JF - Carlsberg Research Communications
SN - 0105-1938
IS - 6
ER -
ID: 204471863