Phylogenetic analysis of F-bZIP transcription factors indicates conservation of the zinc deficiency response across land plants
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Phylogenetic analysis of F-bZIP transcription factors indicates conservation of the zinc deficiency response across land plants. / Castro, Pedro Humberto Araújo R F; Lilay, Grmay Hailu; Muñoz-Mérida, Antonio; Schjørring, Jan Kofod; Azevedo, Herlânder; Assuncao, Ana Goncalves Leite de.
In: Scientific Reports, Vol. 7, No. 1, 3806, 2017.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Phylogenetic analysis of F-bZIP transcription factors indicates conservation of the zinc deficiency response across land plants
AU - Castro, Pedro Humberto Araújo R F
AU - Lilay, Grmay Hailu
AU - Muñoz-Mérida, Antonio
AU - Schjørring, Jan Kofod
AU - Azevedo, Herlânder
AU - Assuncao, Ana Goncalves Leite de
PY - 2017
Y1 - 2017
N2 - Basic leucine zipper (bZIP) transcription factors control important developmental and physiological processes in plants. In Arabidopsis thaliana, the three gene F-bZIP subfamily has been associated with zinc deficiency and salt stress response. Benefiting from the present abundance of plant genomic data, we performed an evolutionary and structural characterization of plant F-bZIPs. We observed divergence during seed plant evolution, into two groups and inferred different selective pressures for each. Group 1 contains AtbZIP19 and AtbZIP23 and appears more conserved, whereas Group 2, containing AtbZIP24, is more prone to gene loss and expansion events. Transcriptomic and experimental data reinforced AtbZIP19/23 as pivotal regulators of the zinc deficiency response, mostly via the activation of genes from the ZIP metal transporter family, and revealed that they are the main regulatory switch of AtZIP4. A survey of AtZIP4 orthologs promoters across different plant taxa revealed an enrichment of the Zinc Deficiency Response Element (ZDRE) to which both AtbZIP19/23 bind. Overall, our results indicate that while the AtbZIP24 function in the regulation of the salt stress response may be the result of neo-functionalization, the AtbZIP19/23 function in the regulation of the zinc deficiency response may be conserved in land plants (Embryophytes).
AB - Basic leucine zipper (bZIP) transcription factors control important developmental and physiological processes in plants. In Arabidopsis thaliana, the three gene F-bZIP subfamily has been associated with zinc deficiency and salt stress response. Benefiting from the present abundance of plant genomic data, we performed an evolutionary and structural characterization of plant F-bZIPs. We observed divergence during seed plant evolution, into two groups and inferred different selective pressures for each. Group 1 contains AtbZIP19 and AtbZIP23 and appears more conserved, whereas Group 2, containing AtbZIP24, is more prone to gene loss and expansion events. Transcriptomic and experimental data reinforced AtbZIP19/23 as pivotal regulators of the zinc deficiency response, mostly via the activation of genes from the ZIP metal transporter family, and revealed that they are the main regulatory switch of AtZIP4. A survey of AtZIP4 orthologs promoters across different plant taxa revealed an enrichment of the Zinc Deficiency Response Element (ZDRE) to which both AtbZIP19/23 bind. Overall, our results indicate that while the AtbZIP24 function in the regulation of the salt stress response may be the result of neo-functionalization, the AtbZIP19/23 function in the regulation of the zinc deficiency response may be conserved in land plants (Embryophytes).
KW - Journal Article
U2 - 10.1038/s41598-017-03903-6
DO - 10.1038/s41598-017-03903-6
M3 - Journal article
C2 - 28630437
VL - 7
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 3806
ER -
ID: 179884734