Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli. / Schulz, Rüdiger; Steinmüller, Klaus; Klaas, Manfred; Forreiter, Christoph; Rasmussen, Sören; Hiller, Claudia; Apel, Klaus.

In: MGG Molecular & General Genetics, Vol. 217, No. 2-3, 01.06.1989, p. 355-361.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Schulz, R, Steinmüller, K, Klaas, M, Forreiter, C, Rasmussen, S, Hiller, C & Apel, K 1989, 'Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli', MGG Molecular & General Genetics, vol. 217, no. 2-3, pp. 355-361. https://doi.org/10.1007/BF02464904

APA

Schulz, R., Steinmüller, K., Klaas, M., Forreiter, C., Rasmussen, S., Hiller, C., & Apel, K. (1989). Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli. MGG Molecular & General Genetics, 217(2-3), 355-361. https://doi.org/10.1007/BF02464904

Vancouver

Schulz R, Steinmüller K, Klaas M, Forreiter C, Rasmussen S, Hiller C et al. Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli. MGG Molecular & General Genetics. 1989 Jun 1;217(2-3):355-361. https://doi.org/10.1007/BF02464904

Author

Schulz, Rüdiger ; Steinmüller, Klaus ; Klaas, Manfred ; Forreiter, Christoph ; Rasmussen, Sören ; Hiller, Claudia ; Apel, Klaus. / Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli. In: MGG Molecular & General Genetics. 1989 ; Vol. 217, No. 2-3. pp. 355-361.

Bibtex

@article{20e0c8b6a7b641d294a9cd1b8862c3b3,
title = "Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli",
abstract = "The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley has been deduced from the nucleotide sequence of a cloned full-length cDNA. This cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings is drastically reduced upon illumination. The predicted amino acid sequence (388 residues in length) includes a transit peptide of 74 amino acids whose end point has been delimited by sequencing the N-terminus of the mature protein. Expression of the cDNA in Escherichia coli leads to the synthesis of an enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase. Activity of this protein in bacterial lysates is completely dependent on the presence of NADPH and protochlorophyllide and requires light.",
keywords = "Barley, cDNA sequence, Light regulation, NADPH-protochlorophyllide oxidoreductase, Transit peptide",
author = "R{\"u}diger Schulz and Klaus Steinm{\"u}ller and Manfred Klaas and Christoph Forreiter and S{\"o}ren Rasmussen and Claudia Hiller and Klaus Apel",
year = "1989",
month = jun,
day = "1",
doi = "10.1007/BF02464904",
language = "English",
volume = "217",
pages = "355--361",
journal = "Molecular General Genetics",
issn = "0026-8925",
publisher = "Springer Verlag",
number = "2-3",

}

RIS

TY - JOUR

T1 - Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli

AU - Schulz, Rüdiger

AU - Steinmüller, Klaus

AU - Klaas, Manfred

AU - Forreiter, Christoph

AU - Rasmussen, Sören

AU - Hiller, Claudia

AU - Apel, Klaus

PY - 1989/6/1

Y1 - 1989/6/1

N2 - The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley has been deduced from the nucleotide sequence of a cloned full-length cDNA. This cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings is drastically reduced upon illumination. The predicted amino acid sequence (388 residues in length) includes a transit peptide of 74 amino acids whose end point has been delimited by sequencing the N-terminus of the mature protein. Expression of the cDNA in Escherichia coli leads to the synthesis of an enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase. Activity of this protein in bacterial lysates is completely dependent on the presence of NADPH and protochlorophyllide and requires light.

AB - The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley has been deduced from the nucleotide sequence of a cloned full-length cDNA. This cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings is drastically reduced upon illumination. The predicted amino acid sequence (388 residues in length) includes a transit peptide of 74 amino acids whose end point has been delimited by sequencing the N-terminus of the mature protein. Expression of the cDNA in Escherichia coli leads to the synthesis of an enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase. Activity of this protein in bacterial lysates is completely dependent on the presence of NADPH and protochlorophyllide and requires light.

KW - Barley

KW - cDNA sequence

KW - Light regulation

KW - NADPH-protochlorophyllide oxidoreductase

KW - Transit peptide

UR - http://www.scopus.com/inward/record.url?scp=0024676061&partnerID=8YFLogxK

U2 - 10.1007/BF02464904

DO - 10.1007/BF02464904

M3 - Journal article

C2 - 2671659

AN - SCOPUS:0024676061

VL - 217

SP - 355

EP - 361

JO - Molecular General Genetics

JF - Molecular General Genetics

SN - 0026-8925

IS - 2-3

ER -

ID: 204471569