Localization of introduced genes on the chromosomes of transgenic barley, wheat and triticale by fluorescence in situ hybridization

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Localization of introduced genes on the chromosomes of transgenic barley, wheat and triticale by fluorescence in situ hybridization. / Pedersen, C.; Zimny, J.; Becker, D.; Jähne-Gärtner, A.; Lörz, H.

In: Theoretical and Applied Genetics, Vol. 94, No. 6-7, 01.05.1997, p. 749-757.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Pedersen, C, Zimny, J, Becker, D, Jähne-Gärtner, A & Lörz, H 1997, 'Localization of introduced genes on the chromosomes of transgenic barley, wheat and triticale by fluorescence in situ hybridization', Theoretical and Applied Genetics, vol. 94, no. 6-7, pp. 749-757. https://doi.org/10.1007/s001220050474

APA

Pedersen, C., Zimny, J., Becker, D., Jähne-Gärtner, A., & Lörz, H. (1997). Localization of introduced genes on the chromosomes of transgenic barley, wheat and triticale by fluorescence in situ hybridization. Theoretical and Applied Genetics, 94(6-7), 749-757. https://doi.org/10.1007/s001220050474

Vancouver

Pedersen C, Zimny J, Becker D, Jähne-Gärtner A, Lörz H. Localization of introduced genes on the chromosomes of transgenic barley, wheat and triticale by fluorescence in situ hybridization. Theoretical and Applied Genetics. 1997 May 1;94(6-7):749-757. https://doi.org/10.1007/s001220050474

Author

Pedersen, C. ; Zimny, J. ; Becker, D. ; Jähne-Gärtner, A. ; Lörz, H. / Localization of introduced genes on the chromosomes of transgenic barley, wheat and triticale by fluorescence in situ hybridization. In: Theoretical and Applied Genetics. 1997 ; Vol. 94, No. 6-7. pp. 749-757.

Bibtex

@article{a93372fcddb6404899a4578be2ebb443,
title = "Localization of introduced genes on the chromosomes of transgenic barley, wheat and triticale by fluorescence in situ hybridization",
abstract = "Using fluorescence in situ hybridization (FISH) we localized introduced genes on metaphase chromosomes of barley, wheat, and triticale transformed by microprojectile bombardment of microspores and scutellar tissue with the pDB1 plasmid containing the uid A and bar genes. Thirteen integration sites were detected in the nine lines analysed. Southern analysis showed that three or more copies of the plasmid were present in the lines. In a triticale line containing four copies three different integration sites were identified, indicating that the method described is sensitive enough for the detection of single-copy integrations. There was a slight tendency towards the localization of transgenes in distal chromosome regions. Using the GAA-satellite sequence for chromosome banding, the chromosomes containing the inserted genes were identified in most cases. Two barley lines derived from the same transformant showed a totally different integration pattern. Southern analysis confirmed that the inserted genes were segregating independently, resulting in different integration patterns among the progeny lines. The application of the FISH technique for the analysis of transgenic plants is discussed.",
keywords = "Chromosomal localization, FISH, Integration pattern, Transgenic barley, Wheat and triticale",
author = "C. Pedersen and J. Zimny and D. Becker and A. J{\"a}hne-G{\"a}rtner and H. L{\"o}rz",
year = "1997",
month = may,
day = "1",
doi = "10.1007/s001220050474",
language = "English",
volume = "94",
pages = "749--757",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer",
number = "6-7",

}

RIS

TY - JOUR

T1 - Localization of introduced genes on the chromosomes of transgenic barley, wheat and triticale by fluorescence in situ hybridization

AU - Pedersen, C.

AU - Zimny, J.

AU - Becker, D.

AU - Jähne-Gärtner, A.

AU - Lörz, H.

PY - 1997/5/1

Y1 - 1997/5/1

N2 - Using fluorescence in situ hybridization (FISH) we localized introduced genes on metaphase chromosomes of barley, wheat, and triticale transformed by microprojectile bombardment of microspores and scutellar tissue with the pDB1 plasmid containing the uid A and bar genes. Thirteen integration sites were detected in the nine lines analysed. Southern analysis showed that three or more copies of the plasmid were present in the lines. In a triticale line containing four copies three different integration sites were identified, indicating that the method described is sensitive enough for the detection of single-copy integrations. There was a slight tendency towards the localization of transgenes in distal chromosome regions. Using the GAA-satellite sequence for chromosome banding, the chromosomes containing the inserted genes were identified in most cases. Two barley lines derived from the same transformant showed a totally different integration pattern. Southern analysis confirmed that the inserted genes were segregating independently, resulting in different integration patterns among the progeny lines. The application of the FISH technique for the analysis of transgenic plants is discussed.

AB - Using fluorescence in situ hybridization (FISH) we localized introduced genes on metaphase chromosomes of barley, wheat, and triticale transformed by microprojectile bombardment of microspores and scutellar tissue with the pDB1 plasmid containing the uid A and bar genes. Thirteen integration sites were detected in the nine lines analysed. Southern analysis showed that three or more copies of the plasmid were present in the lines. In a triticale line containing four copies three different integration sites were identified, indicating that the method described is sensitive enough for the detection of single-copy integrations. There was a slight tendency towards the localization of transgenes in distal chromosome regions. Using the GAA-satellite sequence for chromosome banding, the chromosomes containing the inserted genes were identified in most cases. Two barley lines derived from the same transformant showed a totally different integration pattern. Southern analysis confirmed that the inserted genes were segregating independently, resulting in different integration patterns among the progeny lines. The application of the FISH technique for the analysis of transgenic plants is discussed.

KW - Chromosomal localization

KW - FISH

KW - Integration pattern

KW - Transgenic barley

KW - Wheat and triticale

UR - http://www.scopus.com/inward/record.url?scp=0030955688&partnerID=8YFLogxK

U2 - 10.1007/s001220050474

DO - 10.1007/s001220050474

M3 - Journal article

AN - SCOPUS:0030955688

VL - 94

SP - 749

EP - 757

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 6-7

ER -

ID: 226915036