HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply

Research output: Contribution to journalJournal articlepeer-review

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HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply. / Tiong, Jingwen; Mcdonald, Glenn K.; Genc, Yusuf; Pedas, Pai; Hayes, Julie E.; Toubia, John; Langridge, Peter; Huang, Chun Y.

In: New Phytologist, Vol. 201, No. 1, 2014, p. 131-143.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Tiong, J, Mcdonald, GK, Genc, Y, Pedas, P, Hayes, JE, Toubia, J, Langridge, P & Huang, CY 2014, 'HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply', New Phytologist, vol. 201, no. 1, pp. 131-143. https://doi.org/10.1111/nph.12468

APA

Tiong, J., Mcdonald, G. K., Genc, Y., Pedas, P., Hayes, J. E., Toubia, J., Langridge, P., & Huang, C. Y. (2014). HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply. New Phytologist, 201(1), 131-143. https://doi.org/10.1111/nph.12468

Vancouver

Tiong J, Mcdonald GK, Genc Y, Pedas P, Hayes JE, Toubia J et al. HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply. New Phytologist. 2014;201(1):131-143. https://doi.org/10.1111/nph.12468

Author

Tiong, Jingwen ; Mcdonald, Glenn K. ; Genc, Yusuf ; Pedas, Pai ; Hayes, Julie E. ; Toubia, John ; Langridge, Peter ; Huang, Chun Y. / HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply. In: New Phytologist. 2014 ; Vol. 201, No. 1. pp. 131-143.

Bibtex

@article{d44cf5d66b074cf6be1291b46f481fb8,
title = "HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply",
abstract = "Summary: High expression of zinc (Zn)-regulated, iron-regulated transporter-like protein (ZIP) genes increases root Zn uptake in dicots, leading to high accumulation of Zn in shoots. However, none of the ZIP genes tested previously in monocots could enhance shoot Zn accumulation. In this report, barley (Hordeum vulgare) HvZIP7 was investigated for its functions in Zn transport. The functions of HvZIP7 in planta were studied using in situ hybridization and transient analysis of subcellular localization with a green fluorescent protein (GFP) reporter. Transgenic barley lines overexpressing HvZIP7 were also generated to further understand the functions of HvZIP7 in metal transport. HvZIP7 is strongly induced by Zn deficiency, primarily in vascular tissues of roots and leaves, and its protein was localized in the plasma membrane. These properties are similar to its closely related homologs in dicots. Overexpression of HvZIP7 in barley plants increased Zn uptake when moderately high concentrations of Zn were supplied. Significantly, there was a specific enhancement of shoot Zn accumulation, with no measurable increase in iron (Fe), manganese (Mn), copper (Cu) or cadmium (Cd). HvZIP7 displays characteristics of low-affinity Zn transport. The unique function of HvZIP7 provides new insights into the role of ZIP genes in Zn homeostasis in monocots, and offers opportunities to develop Zn biofortification strategies in cereals.",
author = "Jingwen Tiong and Mcdonald, {Glenn K.} and Yusuf Genc and Pai Pedas and Hayes, {Julie E.} and John Toubia and Peter Langridge and Huang, {Chun Y.}",
year = "2014",
doi = "10.1111/nph.12468",
language = "English",
volume = "201",
pages = "131--143",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply

AU - Tiong, Jingwen

AU - Mcdonald, Glenn K.

AU - Genc, Yusuf

AU - Pedas, Pai

AU - Hayes, Julie E.

AU - Toubia, John

AU - Langridge, Peter

AU - Huang, Chun Y.

PY - 2014

Y1 - 2014

N2 - Summary: High expression of zinc (Zn)-regulated, iron-regulated transporter-like protein (ZIP) genes increases root Zn uptake in dicots, leading to high accumulation of Zn in shoots. However, none of the ZIP genes tested previously in monocots could enhance shoot Zn accumulation. In this report, barley (Hordeum vulgare) HvZIP7 was investigated for its functions in Zn transport. The functions of HvZIP7 in planta were studied using in situ hybridization and transient analysis of subcellular localization with a green fluorescent protein (GFP) reporter. Transgenic barley lines overexpressing HvZIP7 were also generated to further understand the functions of HvZIP7 in metal transport. HvZIP7 is strongly induced by Zn deficiency, primarily in vascular tissues of roots and leaves, and its protein was localized in the plasma membrane. These properties are similar to its closely related homologs in dicots. Overexpression of HvZIP7 in barley plants increased Zn uptake when moderately high concentrations of Zn were supplied. Significantly, there was a specific enhancement of shoot Zn accumulation, with no measurable increase in iron (Fe), manganese (Mn), copper (Cu) or cadmium (Cd). HvZIP7 displays characteristics of low-affinity Zn transport. The unique function of HvZIP7 provides new insights into the role of ZIP genes in Zn homeostasis in monocots, and offers opportunities to develop Zn biofortification strategies in cereals.

AB - Summary: High expression of zinc (Zn)-regulated, iron-regulated transporter-like protein (ZIP) genes increases root Zn uptake in dicots, leading to high accumulation of Zn in shoots. However, none of the ZIP genes tested previously in monocots could enhance shoot Zn accumulation. In this report, barley (Hordeum vulgare) HvZIP7 was investigated for its functions in Zn transport. The functions of HvZIP7 in planta were studied using in situ hybridization and transient analysis of subcellular localization with a green fluorescent protein (GFP) reporter. Transgenic barley lines overexpressing HvZIP7 were also generated to further understand the functions of HvZIP7 in metal transport. HvZIP7 is strongly induced by Zn deficiency, primarily in vascular tissues of roots and leaves, and its protein was localized in the plasma membrane. These properties are similar to its closely related homologs in dicots. Overexpression of HvZIP7 in barley plants increased Zn uptake when moderately high concentrations of Zn were supplied. Significantly, there was a specific enhancement of shoot Zn accumulation, with no measurable increase in iron (Fe), manganese (Mn), copper (Cu) or cadmium (Cd). HvZIP7 displays characteristics of low-affinity Zn transport. The unique function of HvZIP7 provides new insights into the role of ZIP genes in Zn homeostasis in monocots, and offers opportunities to develop Zn biofortification strategies in cereals.

U2 - 10.1111/nph.12468

DO - 10.1111/nph.12468

M3 - Journal article

C2 - 24033183

AN - SCOPUS:84888297662

VL - 201

SP - 131

EP - 143

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 1

ER -

ID: 102106832