High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry

Research output: Contribution to journalJournal articlepeer-review

Standard

High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry. / Dahl-Lassen, Rasmus; van Hecke, Jan Julien Josef; Jørgensen, Henning; Bukh, Christian; Andersen, Birgit; Schjørring, Jan Kofod.

In: Plant Methods, Vol. 14, 8, 2018.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Dahl-Lassen, R, van Hecke, JJJ, Jørgensen, H, Bukh, C, Andersen, B & Schjørring, JK 2018, 'High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry', Plant Methods, vol. 14, 8. https://doi.org/10.1186/s13007-018-0277-8

APA

Dahl-Lassen, R., van Hecke, J. J. J., Jørgensen, H., Bukh, C., Andersen, B., & Schjørring, J. K. (2018). High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry. Plant Methods, 14, [8]. https://doi.org/10.1186/s13007-018-0277-8

Vancouver

Dahl-Lassen R, van Hecke JJJ, Jørgensen H, Bukh C, Andersen B, Schjørring JK. High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry. Plant Methods. 2018;14. 8. https://doi.org/10.1186/s13007-018-0277-8

Author

Dahl-Lassen, Rasmus ; van Hecke, Jan Julien Josef ; Jørgensen, Henning ; Bukh, Christian ; Andersen, Birgit ; Schjørring, Jan Kofod. / High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry. In: Plant Methods. 2018 ; Vol. 14.

Bibtex

@article{91b68f047cc14f4bbf7fbb5a0c56b72d,
title = "High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry",
abstract = "Background: The amino acid profile of plants is an important parameter in assessments of their growth potential, resource-use efficiency and/or quality as food and feed. Screening studies may involve large number of samples but the classical amino acid analysis is limited by the fact that it is very time consuming with typical chromatographic run times of 70 min or more. Results: We have here developed a high-throughput method for analysis of amino acid profiles in plant materials. The method combines classical protein hydrolysis and derivatization with fast separation by UHPLC and detection by a single quadrupole (QDa) mass spectrometer. The chromatographic run time is reduced to 10 min and the precision, accuracy and sensitivity of the method are in line with other recent methods utilizing advanced and more expensive mass spectrometers. The sensitivity of the method is at least a factor 10 better than that of methods relying on detection by fluorescence or UV. It is possible to downscale sample size to 20 mg without compromising reproducibility, which makes the method ideal for analysis of very small sample amounts. Conclusion: The developed method allows high-throughput analysis of amino acid profiles in plant materials. The analysis is robust and accurate as well as compatible with both free amino acids and protein hydrolysates. The QDa detector offers high sensitivity and accuracy, while at the same time being relatively simple to operate and cheap to purchase, thus significantly reducing the overall analytical costs compared to methods based on more advanced mass spectrometers.",
keywords = "Amino acid analysis, Biomass, Biorefinery, Green leaves, Mass spectrometry, Protein extraction",
author = "Rasmus Dahl-Lassen and {van Hecke}, {Jan Julien Josef} and Henning J{\o}rgensen and Christian Bukh and Birgit Andersen and Schj{\o}rring, {Jan Kofod}",
year = "2018",
doi = "10.1186/s13007-018-0277-8",
language = "English",
volume = "14",
journal = "Plant Methods",
issn = "1746-4811",
publisher = "BioMed Central",

}

RIS

TY - JOUR

T1 - High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry

AU - Dahl-Lassen, Rasmus

AU - van Hecke, Jan Julien Josef

AU - Jørgensen, Henning

AU - Bukh, Christian

AU - Andersen, Birgit

AU - Schjørring, Jan Kofod

PY - 2018

Y1 - 2018

N2 - Background: The amino acid profile of plants is an important parameter in assessments of their growth potential, resource-use efficiency and/or quality as food and feed. Screening studies may involve large number of samples but the classical amino acid analysis is limited by the fact that it is very time consuming with typical chromatographic run times of 70 min or more. Results: We have here developed a high-throughput method for analysis of amino acid profiles in plant materials. The method combines classical protein hydrolysis and derivatization with fast separation by UHPLC and detection by a single quadrupole (QDa) mass spectrometer. The chromatographic run time is reduced to 10 min and the precision, accuracy and sensitivity of the method are in line with other recent methods utilizing advanced and more expensive mass spectrometers. The sensitivity of the method is at least a factor 10 better than that of methods relying on detection by fluorescence or UV. It is possible to downscale sample size to 20 mg without compromising reproducibility, which makes the method ideal for analysis of very small sample amounts. Conclusion: The developed method allows high-throughput analysis of amino acid profiles in plant materials. The analysis is robust and accurate as well as compatible with both free amino acids and protein hydrolysates. The QDa detector offers high sensitivity and accuracy, while at the same time being relatively simple to operate and cheap to purchase, thus significantly reducing the overall analytical costs compared to methods based on more advanced mass spectrometers.

AB - Background: The amino acid profile of plants is an important parameter in assessments of their growth potential, resource-use efficiency and/or quality as food and feed. Screening studies may involve large number of samples but the classical amino acid analysis is limited by the fact that it is very time consuming with typical chromatographic run times of 70 min or more. Results: We have here developed a high-throughput method for analysis of amino acid profiles in plant materials. The method combines classical protein hydrolysis and derivatization with fast separation by UHPLC and detection by a single quadrupole (QDa) mass spectrometer. The chromatographic run time is reduced to 10 min and the precision, accuracy and sensitivity of the method are in line with other recent methods utilizing advanced and more expensive mass spectrometers. The sensitivity of the method is at least a factor 10 better than that of methods relying on detection by fluorescence or UV. It is possible to downscale sample size to 20 mg without compromising reproducibility, which makes the method ideal for analysis of very small sample amounts. Conclusion: The developed method allows high-throughput analysis of amino acid profiles in plant materials. The analysis is robust and accurate as well as compatible with both free amino acids and protein hydrolysates. The QDa detector offers high sensitivity and accuracy, while at the same time being relatively simple to operate and cheap to purchase, thus significantly reducing the overall analytical costs compared to methods based on more advanced mass spectrometers.

KW - Amino acid analysis

KW - Biomass

KW - Biorefinery

KW - Green leaves

KW - Mass spectrometry

KW - Protein extraction

U2 - 10.1186/s13007-018-0277-8

DO - 10.1186/s13007-018-0277-8

M3 - Journal article

C2 - 29375649

AN - SCOPUS:85040795931

VL - 14

JO - Plant Methods

JF - Plant Methods

SN - 1746-4811

M1 - 8

ER -

ID: 196737347