Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley

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Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley. / Pedersen, Carsten; Linde-Laursen, Ib.

In: Chromosome Research, Vol. 2, No. 1, 01.01.1994, p. 65-71.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pedersen, C & Linde-Laursen, I 1994, 'Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley', Chromosome Research, vol. 2, no. 1, pp. 65-71. https://doi.org/10.1007/BF01539456

APA

Pedersen, C., & Linde-Laursen, I. (1994). Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley. Chromosome Research, 2(1), 65-71. https://doi.org/10.1007/BF01539456

Vancouver

Pedersen C, Linde-Laursen I. Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley. Chromosome Research. 1994 Jan 1;2(1):65-71. https://doi.org/10.1007/BF01539456

Author

Pedersen, Carsten ; Linde-Laursen, Ib. / Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley. In: Chromosome Research. 1994 ; Vol. 2, No. 1. pp. 65-71.

Bibtex

@article{2a49bb8fdaec4118b38ff7072648bfcf,
title = "Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley",
abstract = "Four minor rDNA loci have been mapped physically to barley (Hordeum vulgare L.) chromosomes 1 (7I), 2 (2I), 4 (4I), and 5 (1I) by a two-step in situ hybridization procedure including a GAA microsatellite sequence. Reprobing with the microsatellite resulted in a distinct banding pattern, resembling the C-banding pattern, which enabled unequivocal chromosome identification. This study suggests that gene mapping accuracy may be improved by using probes with well-characterized and narrow hybridization sites as cytological markers which are situated close to the gene locus. One of the rDNA loci is located about 54% out on the short arm of chromosome 4 and it has not previously been reported in barley. We have disignated the new locus Nor-16. rDNA loci on homoeologous group 4 chromosomes have not yet been reported in other Triticeae species. The origin of these 4 minor rDNA loci is discussed in relation to their equilocal distribution on the chromosomes.",
keywords = "C-banding, chromosome identification, Hordeum vulgare, in situ hybridization, microsatellite",
author = "Carsten Pedersen and Ib Linde-Laursen",
year = "1994",
month = jan,
day = "1",
doi = "10.1007/BF01539456",
language = "English",
volume = "2",
pages = "65--71",
journal = "Chromosome Research",
issn = "0967-3849",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley

AU - Pedersen, Carsten

AU - Linde-Laursen, Ib

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Four minor rDNA loci have been mapped physically to barley (Hordeum vulgare L.) chromosomes 1 (7I), 2 (2I), 4 (4I), and 5 (1I) by a two-step in situ hybridization procedure including a GAA microsatellite sequence. Reprobing with the microsatellite resulted in a distinct banding pattern, resembling the C-banding pattern, which enabled unequivocal chromosome identification. This study suggests that gene mapping accuracy may be improved by using probes with well-characterized and narrow hybridization sites as cytological markers which are situated close to the gene locus. One of the rDNA loci is located about 54% out on the short arm of chromosome 4 and it has not previously been reported in barley. We have disignated the new locus Nor-16. rDNA loci on homoeologous group 4 chromosomes have not yet been reported in other Triticeae species. The origin of these 4 minor rDNA loci is discussed in relation to their equilocal distribution on the chromosomes.

AB - Four minor rDNA loci have been mapped physically to barley (Hordeum vulgare L.) chromosomes 1 (7I), 2 (2I), 4 (4I), and 5 (1I) by a two-step in situ hybridization procedure including a GAA microsatellite sequence. Reprobing with the microsatellite resulted in a distinct banding pattern, resembling the C-banding pattern, which enabled unequivocal chromosome identification. This study suggests that gene mapping accuracy may be improved by using probes with well-characterized and narrow hybridization sites as cytological markers which are situated close to the gene locus. One of the rDNA loci is located about 54% out on the short arm of chromosome 4 and it has not previously been reported in barley. We have disignated the new locus Nor-16. rDNA loci on homoeologous group 4 chromosomes have not yet been reported in other Triticeae species. The origin of these 4 minor rDNA loci is discussed in relation to their equilocal distribution on the chromosomes.

KW - C-banding

KW - chromosome identification

KW - Hordeum vulgare

KW - in situ hybridization

KW - microsatellite

UR - http://www.scopus.com/inward/record.url?scp=0028121052&partnerID=8YFLogxK

U2 - 10.1007/BF01539456

DO - 10.1007/BF01539456

M3 - Journal article

C2 - 8162323

AN - SCOPUS:0028121052

VL - 2

SP - 65

EP - 71

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

IS - 1

ER -

ID: 226915656