Arabidopsis phospholipase Dδ is involved in basal defense and nonhost resistance to powdery mildew fungi

Research output: Contribution to journalJournal articlepeer-review

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Arabidopsis phospholipase Dδ is involved in basal defense and nonhost resistance to powdery mildew fungi. / Pinosa, Francesco; Buhot, Nathalie; Kwaaitaal, Mark Adrianus Cornelis J; Fahlberg, Per; Thordal-Christensen, Hans; Ellerström, Mats; Andersson, Mats X.

In: Plant Physiology, Vol. 163, No. 2, 2013, p. 896-906.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Pinosa, F, Buhot, N, Kwaaitaal, MACJ, Fahlberg, P, Thordal-Christensen, H, Ellerström, M & Andersson, MX 2013, 'Arabidopsis phospholipase Dδ is involved in basal defense and nonhost resistance to powdery mildew fungi', Plant Physiology, vol. 163, no. 2, pp. 896-906. https://doi.org/10.1104/pp.113.223503

APA

Pinosa, F., Buhot, N., Kwaaitaal, M. A. C. J., Fahlberg, P., Thordal-Christensen, H., Ellerström, M., & Andersson, M. X. (2013). Arabidopsis phospholipase Dδ is involved in basal defense and nonhost resistance to powdery mildew fungi. Plant Physiology, 163(2), 896-906. https://doi.org/10.1104/pp.113.223503

Vancouver

Pinosa F, Buhot N, Kwaaitaal MACJ, Fahlberg P, Thordal-Christensen H, Ellerström M et al. Arabidopsis phospholipase Dδ is involved in basal defense and nonhost resistance to powdery mildew fungi. Plant Physiology. 2013;163(2):896-906. https://doi.org/10.1104/pp.113.223503

Author

Pinosa, Francesco ; Buhot, Nathalie ; Kwaaitaal, Mark Adrianus Cornelis J ; Fahlberg, Per ; Thordal-Christensen, Hans ; Ellerström, Mats ; Andersson, Mats X. / Arabidopsis phospholipase Dδ is involved in basal defense and nonhost resistance to powdery mildew fungi. In: Plant Physiology. 2013 ; Vol. 163, No. 2. pp. 896-906.

Bibtex

@article{04f6c082f75e4146a2aea5d8e949bb9a,
title = "Arabidopsis phospholipase Dδ is involved in basal defense and nonhost resistance to powdery mildew fungi",
abstract = "Plants have evolved a complex array of defensive responses against pathogenic microorganisms. Recognition of microbes initiates signaling cascades that activate plant defenses. The membrane lipid phosphatidic acid, produced by phospholipase D (PLD), has been shown to take part in both abiotic and biotic stress signaling. In this study, the involvement of PLD in the interaction between Arabidopsis (Arabidopsis thaliana) and the barley powdery mildew fungus Blumeria graminis f. sp. hordei (Bgh) was investigated. This nonadapted pathogen is normally resisted by a cell wall-based defense, which stops the fungal hyphae from penetrating the epidermal cell wall. Chemical inhibition of phosphatidic acid production by PLD increased the penetration rate of Bgh spores on wild-type leaves. The analysis of transfer DNA knockout lines for all Arabidopsis PLD genes revealed that PLDδ is involved in penetration resistance against Bgh, and chemical inhibition of PLDs in plants mutated in PLDδ indicated that this isoform alone is involved in Bgh resistance. In addition, we confirmed the involvement of PLDδ in penetration resistance against another nonadapted pea powdery mildew fungus, Erysiphe pisi. A green fluorescent protein fusion of PLDδ localized to the plasma membrane at the Bgh attack site, where it surrounded the cell wall reinforcement. Furthermore, in the pldδ mutant, transcriptional up-regulation of early microbe-associated molecular pattern response genes was delayed after chitin stimulation. In conclusion, we propose that PLD is involved in defense signaling in nonhost resistance against powdery mildew fungi and put PLDδ forward as the main isoform participating in this process.",
author = "Francesco Pinosa and Nathalie Buhot and Kwaaitaal, {Mark Adrianus Cornelis J} and Per Fahlberg and Hans Thordal-Christensen and Mats Ellerstr{\"o}m and Andersson, {Mats X.}",
year = "2013",
doi = "10.1104/pp.113.223503",
language = "English",
volume = "163",
pages = "896--906",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "2",

}

RIS

TY - JOUR

T1 - Arabidopsis phospholipase Dδ is involved in basal defense and nonhost resistance to powdery mildew fungi

AU - Pinosa, Francesco

AU - Buhot, Nathalie

AU - Kwaaitaal, Mark Adrianus Cornelis J

AU - Fahlberg, Per

AU - Thordal-Christensen, Hans

AU - Ellerström, Mats

AU - Andersson, Mats X.

PY - 2013

Y1 - 2013

N2 - Plants have evolved a complex array of defensive responses against pathogenic microorganisms. Recognition of microbes initiates signaling cascades that activate plant defenses. The membrane lipid phosphatidic acid, produced by phospholipase D (PLD), has been shown to take part in both abiotic and biotic stress signaling. In this study, the involvement of PLD in the interaction between Arabidopsis (Arabidopsis thaliana) and the barley powdery mildew fungus Blumeria graminis f. sp. hordei (Bgh) was investigated. This nonadapted pathogen is normally resisted by a cell wall-based defense, which stops the fungal hyphae from penetrating the epidermal cell wall. Chemical inhibition of phosphatidic acid production by PLD increased the penetration rate of Bgh spores on wild-type leaves. The analysis of transfer DNA knockout lines for all Arabidopsis PLD genes revealed that PLDδ is involved in penetration resistance against Bgh, and chemical inhibition of PLDs in plants mutated in PLDδ indicated that this isoform alone is involved in Bgh resistance. In addition, we confirmed the involvement of PLDδ in penetration resistance against another nonadapted pea powdery mildew fungus, Erysiphe pisi. A green fluorescent protein fusion of PLDδ localized to the plasma membrane at the Bgh attack site, where it surrounded the cell wall reinforcement. Furthermore, in the pldδ mutant, transcriptional up-regulation of early microbe-associated molecular pattern response genes was delayed after chitin stimulation. In conclusion, we propose that PLD is involved in defense signaling in nonhost resistance against powdery mildew fungi and put PLDδ forward as the main isoform participating in this process.

AB - Plants have evolved a complex array of defensive responses against pathogenic microorganisms. Recognition of microbes initiates signaling cascades that activate plant defenses. The membrane lipid phosphatidic acid, produced by phospholipase D (PLD), has been shown to take part in both abiotic and biotic stress signaling. In this study, the involvement of PLD in the interaction between Arabidopsis (Arabidopsis thaliana) and the barley powdery mildew fungus Blumeria graminis f. sp. hordei (Bgh) was investigated. This nonadapted pathogen is normally resisted by a cell wall-based defense, which stops the fungal hyphae from penetrating the epidermal cell wall. Chemical inhibition of phosphatidic acid production by PLD increased the penetration rate of Bgh spores on wild-type leaves. The analysis of transfer DNA knockout lines for all Arabidopsis PLD genes revealed that PLDδ is involved in penetration resistance against Bgh, and chemical inhibition of PLDs in plants mutated in PLDδ indicated that this isoform alone is involved in Bgh resistance. In addition, we confirmed the involvement of PLDδ in penetration resistance against another nonadapted pea powdery mildew fungus, Erysiphe pisi. A green fluorescent protein fusion of PLDδ localized to the plasma membrane at the Bgh attack site, where it surrounded the cell wall reinforcement. Furthermore, in the pldδ mutant, transcriptional up-regulation of early microbe-associated molecular pattern response genes was delayed after chitin stimulation. In conclusion, we propose that PLD is involved in defense signaling in nonhost resistance against powdery mildew fungi and put PLDδ forward as the main isoform participating in this process.

UR - http://www.scopus.com/inward/record.url?scp=84885223321&partnerID=8YFLogxK

U2 - 10.1104/pp.113.223503

DO - 10.1104/pp.113.223503

M3 - Journal article

AN - SCOPUS:84885223321

VL - 163

SP - 896

EP - 906

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 2

ER -

ID: 102230240