Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses. / Nafisi, Majse; Stranne, Maria; Fimognari, Lorenzo; Atwell, Susanna; Martens, Helle Juel; Pedas, Pai Rosager; Hansen, Sara Fasmer; Nawrath, Christiane; Scheller, Henrik V.; Kliebenstein, Daniel James; Sakuragi, Yumiko.

In: Frontiers in Plant Science, Vol. 6, 550, 2015.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Nafisi, M, Stranne, M, Fimognari, L, Atwell, S, Martens, HJ, Pedas, PR, Hansen, SF, Nawrath, C, Scheller, HV, Kliebenstein, DJ & Sakuragi, Y 2015, 'Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses', Frontiers in Plant Science, vol. 6, 550. https://doi.org/10.3389/fpls.2015.00550

APA

Nafisi, M., Stranne, M., Fimognari, L., Atwell, S., Martens, H. J., Pedas, P. R., ... Sakuragi, Y. (2015). Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses. Frontiers in Plant Science, 6, [550]. https://doi.org/10.3389/fpls.2015.00550

Vancouver

Nafisi M, Stranne M, Fimognari L, Atwell S, Martens HJ, Pedas PR et al. Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses. Frontiers in Plant Science. 2015;6. 550. https://doi.org/10.3389/fpls.2015.00550

Author

Nafisi, Majse ; Stranne, Maria ; Fimognari, Lorenzo ; Atwell, Susanna ; Martens, Helle Juel ; Pedas, Pai Rosager ; Hansen, Sara Fasmer ; Nawrath, Christiane ; Scheller, Henrik V. ; Kliebenstein, Daniel James ; Sakuragi, Yumiko. / Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses. In: Frontiers in Plant Science. 2015 ; Vol. 6.

Bibtex

@article{45d34d37368542479a5eefb43cb756fc,
title = "Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses",
abstract = "The epidermis on leaves protects plants from pathogen invasion and provides a waterproof barrier. It consists of a layer of cells that is surrounded by thick cell walls, which are partially impregnated by highly hydrophobic cuticular components. We show that the Arabidopsis T-DNA insertion mutants of REDUCED WALL ACETYLATION 2 (rwa2), previously identified as having reduced O-acetylation of both pectins and hemicelluloses, exhibit pleiotrophic phenotype on the leaf surface. The cuticle layer appeared diffused and was significantly thicker and underneath cell wall layer was interspersed with electron-dense deposits. A large number of trichomes were collapsed and surface permeability of the leaves was enhanced in rwa2 as compared to the wild type. A massive reprogramming of the transcriptome was observed in rwa2 as compared to the wild type, including a coordinated up-regulation of genes involved in responses to abiotic stress, particularly detoxification of reactive oxygen species and defense against microbial pathogens (e.g., lipid transfer proteins, peroxidases). In accordance, peroxidase activities were found to be elevated in rwa2 as compared to the wild type. These results indicate that cell wall acetylation is essential for maintaining the structural integrity of leaf epidermis, and that reduction of cell wall acetylation leads to global stress responses in Arabidopsis.",
author = "Majse Nafisi and Maria Stranne and Lorenzo Fimognari and Susanna Atwell and Martens, {Helle Juel} and Pedas, {Pai Rosager} and Hansen, {Sara Fasmer} and Christiane Nawrath and Scheller, {Henrik V.} and Kliebenstein, {Daniel James} and Yumiko Sakuragi",
year = "2015",
doi = "10.3389/fpls.2015.00550",
language = "English",
volume = "6",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses

AU - Nafisi, Majse

AU - Stranne, Maria

AU - Fimognari, Lorenzo

AU - Atwell, Susanna

AU - Martens, Helle Juel

AU - Pedas, Pai Rosager

AU - Hansen, Sara Fasmer

AU - Nawrath, Christiane

AU - Scheller, Henrik V.

AU - Kliebenstein, Daniel James

AU - Sakuragi, Yumiko

PY - 2015

Y1 - 2015

N2 - The epidermis on leaves protects plants from pathogen invasion and provides a waterproof barrier. It consists of a layer of cells that is surrounded by thick cell walls, which are partially impregnated by highly hydrophobic cuticular components. We show that the Arabidopsis T-DNA insertion mutants of REDUCED WALL ACETYLATION 2 (rwa2), previously identified as having reduced O-acetylation of both pectins and hemicelluloses, exhibit pleiotrophic phenotype on the leaf surface. The cuticle layer appeared diffused and was significantly thicker and underneath cell wall layer was interspersed with electron-dense deposits. A large number of trichomes were collapsed and surface permeability of the leaves was enhanced in rwa2 as compared to the wild type. A massive reprogramming of the transcriptome was observed in rwa2 as compared to the wild type, including a coordinated up-regulation of genes involved in responses to abiotic stress, particularly detoxification of reactive oxygen species and defense against microbial pathogens (e.g., lipid transfer proteins, peroxidases). In accordance, peroxidase activities were found to be elevated in rwa2 as compared to the wild type. These results indicate that cell wall acetylation is essential for maintaining the structural integrity of leaf epidermis, and that reduction of cell wall acetylation leads to global stress responses in Arabidopsis.

AB - The epidermis on leaves protects plants from pathogen invasion and provides a waterproof barrier. It consists of a layer of cells that is surrounded by thick cell walls, which are partially impregnated by highly hydrophobic cuticular components. We show that the Arabidopsis T-DNA insertion mutants of REDUCED WALL ACETYLATION 2 (rwa2), previously identified as having reduced O-acetylation of both pectins and hemicelluloses, exhibit pleiotrophic phenotype on the leaf surface. The cuticle layer appeared diffused and was significantly thicker and underneath cell wall layer was interspersed with electron-dense deposits. A large number of trichomes were collapsed and surface permeability of the leaves was enhanced in rwa2 as compared to the wild type. A massive reprogramming of the transcriptome was observed in rwa2 as compared to the wild type, including a coordinated up-regulation of genes involved in responses to abiotic stress, particularly detoxification of reactive oxygen species and defense against microbial pathogens (e.g., lipid transfer proteins, peroxidases). In accordance, peroxidase activities were found to be elevated in rwa2 as compared to the wild type. These results indicate that cell wall acetylation is essential for maintaining the structural integrity of leaf epidermis, and that reduction of cell wall acetylation leads to global stress responses in Arabidopsis.

U2 - 10.3389/fpls.2015.00550

DO - 10.3389/fpls.2015.00550

M3 - Journal article

C2 - 26257757

VL - 6

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 550

ER -

ID: 146776862