A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum
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The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identified as a member of the 90 kDa heat shock protein (HSP90) family. The encoded protein most resembles the endoplasmic reticulum (ER) resident HSP90 protein, the 94 kDa glucose-regulated protein (GRP94) of vertebrates, as it possesses both the characteristic N-terminal domain including a signal peptide sequence and the C-terminal ER retention signal (Lys-Asp-Glu-Leu). A transcript cross-hybridizing at high stringency accumulated rapidly in leaves upon heat shock treatment. Genomic DNA blot analysis indicated the presence of a family of related genes in the barley genome.
|Journal||Plant Molecular Biology|
|Number of pages||12|
|Publication status||Published - 1 Mar 1993|
- cDNA clone, defence response, Erysiphe graminis, GRP94, heat shock, Hordeum vulgare