Brachypodium distachyon mutants
The TILLING platform for Brachypodium distachyon Bd21-3 has been established containing 3800 mutant lines. The seeds were treated with sodium azide and germinated. Propagation and harvest of plant material is outlined in figure 1. DNA has been isolated from 3000 mutant lines ready for TILLING. We have also prepared a small number (300 lines) of fast neutron mutants.
TILLING in Brachypodium
At the moment TILLING is performed on the Brachypodium mutant population. The mutants are easily identified by ABI Genetic Analyser (16 capillary, autosampler) and confirmed by sequencing (Figure 2). Ph.D.-student Louise Flagstad / Assoc. prof. Anna Maria Torp
Contact Prof. Søren K. Rasmussen for availability of the mutant population and access to the TILLING platform.