The Endo-Arabinanase BcAra1 Is a Novel Host-Specific Virulence Factor of the Necrotic Fungal Phytopathogen Botrytis cinerea

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

The Endo-Arabinanase BcAra1 Is a Novel Host-Specific Virulence Factor of the Necrotic Fungal Phytopathogen Botrytis cinerea. / Nafisi, Majse; Stranne, Maria; Zhang, Lisha; van Kan, Jan A. L.; Sakuragi, Yumiko.

In: Molecular Plant - Microbe Interactions, Vol. 27, No. 8, 01.08.2014, p. 781-792.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Nafisi, M, Stranne, M, Zhang, L, van Kan, JAL & Sakuragi, Y 2014, 'The Endo-Arabinanase BcAra1 Is a Novel Host-Specific Virulence Factor of the Necrotic Fungal Phytopathogen Botrytis cinerea', Molecular Plant - Microbe Interactions, vol. 27, no. 8, pp. 781-792. https://doi.org/10.1094/MPMI-02-14-0036-R

APA

Nafisi, M., Stranne, M., Zhang, L., van Kan, J. A. L., & Sakuragi, Y. (2014). The Endo-Arabinanase BcAra1 Is a Novel Host-Specific Virulence Factor of the Necrotic Fungal Phytopathogen Botrytis cinerea. Molecular Plant - Microbe Interactions, 27(8), 781-792. https://doi.org/10.1094/MPMI-02-14-0036-R

Vancouver

Nafisi M, Stranne M, Zhang L, van Kan JAL, Sakuragi Y. The Endo-Arabinanase BcAra1 Is a Novel Host-Specific Virulence Factor of the Necrotic Fungal Phytopathogen Botrytis cinerea. Molecular Plant - Microbe Interactions. 2014 Aug 1;27(8):781-792. https://doi.org/10.1094/MPMI-02-14-0036-R

Author

Nafisi, Majse ; Stranne, Maria ; Zhang, Lisha ; van Kan, Jan A. L. ; Sakuragi, Yumiko. / The Endo-Arabinanase BcAra1 Is a Novel Host-Specific Virulence Factor of the Necrotic Fungal Phytopathogen Botrytis cinerea. In: Molecular Plant - Microbe Interactions. 2014 ; Vol. 27, No. 8. pp. 781-792.

Bibtex

@article{1eb6cab4c67d4845804133cbad4910f0,
title = "The Endo-Arabinanase BcAra1 Is a Novel Host-Specific Virulence Factor of the Necrotic Fungal Phytopathogen Botrytis cinerea",
abstract = "The plant cell wall is one of the first physical interfaces encountered by plant pathogens and consists of polysaccharides, of which arabinan is an important constituent. During infection, the necrotrophic plant pathogen Botrytis cinerea secretes a cocktail of plant cell-wall-degrading enzymes, including endo-arabinanase activity, which carries out the breakdown of arabinan. The roles of arabinan and endo-arabinanases during microbial infection were thus far elusive. In this study, the gene Bcara1 encoding for a novel α-1,5-L-endo-arabinanase was identified and the heterologously expressed BcAra1 protein was shown to hydrolyze linear arabinan with high efficiency whereas little or no activity was observed against the other oligo- and polysaccharides tested. The Bcara1 knockout mutants displayed reduced arabinanase activity in vitro and severe retardation in secondary lesion formation during infection of Arabidopsis leaves. These results indicate that BcAra1 is a novel endo-arabinanase and plays an important role during the infection of Arabidopsis. Interestingly, the level of Bcara1 transcript was considerably lower during the infection of Nicotiana benthamiana compared with Arabidopsis and, consequently, the ΔBcara1 mutants showed the wild-type level of virulence on N. benthamiana leaves. These results support the conclusion that the expression of Bcara1 is host dependent and is a key determinant of the disease outcome.",
author = "Majse Nafisi and Maria Stranne and Lisha Zhang and {van Kan}, {Jan A. L.} and Yumiko Sakuragi",
year = "2014",
month = "8",
day = "1",
doi = "10.1094/MPMI-02-14-0036-R",
language = "English",
volume = "27",
pages = "781--792",
journal = "Molecular Plant - Microbe Interactions",
issn = "0894-0282",
publisher = "American Phytopathological Society",
number = "8",

}

RIS

TY - JOUR

T1 - The Endo-Arabinanase BcAra1 Is a Novel Host-Specific Virulence Factor of the Necrotic Fungal Phytopathogen Botrytis cinerea

AU - Nafisi, Majse

AU - Stranne, Maria

AU - Zhang, Lisha

AU - van Kan, Jan A. L.

AU - Sakuragi, Yumiko

PY - 2014/8/1

Y1 - 2014/8/1

N2 - The plant cell wall is one of the first physical interfaces encountered by plant pathogens and consists of polysaccharides, of which arabinan is an important constituent. During infection, the necrotrophic plant pathogen Botrytis cinerea secretes a cocktail of plant cell-wall-degrading enzymes, including endo-arabinanase activity, which carries out the breakdown of arabinan. The roles of arabinan and endo-arabinanases during microbial infection were thus far elusive. In this study, the gene Bcara1 encoding for a novel α-1,5-L-endo-arabinanase was identified and the heterologously expressed BcAra1 protein was shown to hydrolyze linear arabinan with high efficiency whereas little or no activity was observed against the other oligo- and polysaccharides tested. The Bcara1 knockout mutants displayed reduced arabinanase activity in vitro and severe retardation in secondary lesion formation during infection of Arabidopsis leaves. These results indicate that BcAra1 is a novel endo-arabinanase and plays an important role during the infection of Arabidopsis. Interestingly, the level of Bcara1 transcript was considerably lower during the infection of Nicotiana benthamiana compared with Arabidopsis and, consequently, the ΔBcara1 mutants showed the wild-type level of virulence on N. benthamiana leaves. These results support the conclusion that the expression of Bcara1 is host dependent and is a key determinant of the disease outcome.

AB - The plant cell wall is one of the first physical interfaces encountered by plant pathogens and consists of polysaccharides, of which arabinan is an important constituent. During infection, the necrotrophic plant pathogen Botrytis cinerea secretes a cocktail of plant cell-wall-degrading enzymes, including endo-arabinanase activity, which carries out the breakdown of arabinan. The roles of arabinan and endo-arabinanases during microbial infection were thus far elusive. In this study, the gene Bcara1 encoding for a novel α-1,5-L-endo-arabinanase was identified and the heterologously expressed BcAra1 protein was shown to hydrolyze linear arabinan with high efficiency whereas little or no activity was observed against the other oligo- and polysaccharides tested. The Bcara1 knockout mutants displayed reduced arabinanase activity in vitro and severe retardation in secondary lesion formation during infection of Arabidopsis leaves. These results indicate that BcAra1 is a novel endo-arabinanase and plays an important role during the infection of Arabidopsis. Interestingly, the level of Bcara1 transcript was considerably lower during the infection of Nicotiana benthamiana compared with Arabidopsis and, consequently, the ΔBcara1 mutants showed the wild-type level of virulence on N. benthamiana leaves. These results support the conclusion that the expression of Bcara1 is host dependent and is a key determinant of the disease outcome.

U2 - 10.1094/MPMI-02-14-0036-R

DO - 10.1094/MPMI-02-14-0036-R

M3 - Journal article

C2 - 24725206

VL - 27

SP - 781

EP - 792

JO - Molecular Plant - Microbe Interactions

JF - Molecular Plant - Microbe Interactions

SN - 0894-0282

IS - 8

ER -

ID: 140013469