Forty-three sweet sorghum accessions were grown in two contrasting environments; Nigeria (tropical environment) and Denmark (temperate environment). The objectives were to determine the interaction between
genotype and environment on grain yield, fresh biomass and stem sugar, and to assess yield stability of sweet sorghum and identify the best genotypes for biofuel production. The sweet sorghum originating from a Dutch and ICRISAT collection was grown in randomized complete block design in three replicates for two years (2014 and 2015). The combined analysis of variance of the sweet sorghum genotypes in two years over the two contrasting environments revealed that year (Y), genotype (G), environment (E) and genotype by environment
interaction (GEI) were significant in the entire biofuel yield attributes obtained from both Dutch and ICRISAT collections except the degree of Brix and fresh biomass respectively across the year. The year and genotype interaction (Y×G) was not significant in all the biofuel attributes of Dutch accessions. Additive main effect and multiplicative interaction (AMMI) analysis of variance showed significant effect of G, E and the GEI. The AMMI was used to identify the best performing, adaptable and more stable genotypes. Twenty-two genotypes of
both ICRISAT and Dutch accessions were identified to be stable across the two locations with respect to different biofuel attributes. Nine, seven, and six genotypes were found to be stable for grain yield, biomass yield
and brix value, respectively. The best performing genotypes for stem sugar across locations were identified. From the available data collected, the performance of the sweet sorghum was attributed to both genetic and
environmental effects. High GE was observed to influence stability, hence will influence the selection criteria of the sweet sorghum genotypes.