Role of the Colletotrichum acutatum sesquiterpene synthase CaTPS in the biosynthesis of sesquiterpenoids

Research output: Contribution to journalJournal articlepeer-review

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Role of the Colletotrichum acutatum sesquiterpene synthase CaTPS in the biosynthesis of sesquiterpenoids. / Amby, Daniel Buchvaldt; Manczak, Tom; Petersen, Mikael Agerlin; Sundelin, Thomas; Weitzel, Corinna; Grajewski, Maciej; Simonsen, Henrik Toft; Jensen, Birgit.

In: Microbiology, Vol. 162, No. 10, 2016, p. 1773-1783.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Amby, DB, Manczak, T, Petersen, MA, Sundelin, T, Weitzel, C, Grajewski, M, Simonsen, HT & Jensen, B 2016, 'Role of the Colletotrichum acutatum sesquiterpene synthase CaTPS in the biosynthesis of sesquiterpenoids', Microbiology, vol. 162, no. 10, pp. 1773-1783. https://doi.org/10.1099/mic.0.000361

APA

Amby, D. B., Manczak, T., Petersen, M. A., Sundelin, T., Weitzel, C., Grajewski, M., Simonsen, H. T., & Jensen, B. (2016). Role of the Colletotrichum acutatum sesquiterpene synthase CaTPS in the biosynthesis of sesquiterpenoids. Microbiology, 162(10), 1773-1783. https://doi.org/10.1099/mic.0.000361

Vancouver

Amby DB, Manczak T, Petersen MA, Sundelin T, Weitzel C, Grajewski M et al. Role of the Colletotrichum acutatum sesquiterpene synthase CaTPS in the biosynthesis of sesquiterpenoids. Microbiology. 2016;162(10):1773-1783. https://doi.org/10.1099/mic.0.000361

Author

Amby, Daniel Buchvaldt ; Manczak, Tom ; Petersen, Mikael Agerlin ; Sundelin, Thomas ; Weitzel, Corinna ; Grajewski, Maciej ; Simonsen, Henrik Toft ; Jensen, Birgit. / Role of the Colletotrichum acutatum sesquiterpene synthase CaTPS in the biosynthesis of sesquiterpenoids. In: Microbiology. 2016 ; Vol. 162, No. 10. pp. 1773-1783.

Bibtex

@article{6ef80bc3a0d54107be648b62d8714b35,
title = "Role of the Colletotrichum acutatum sesquiterpene synthase CaTPS in the biosynthesis of sesquiterpenoids",
abstract = "Colletotrichum acutatum is a major fungal pathogen of fruit crops, which causes severe yield losses in strawberry productions. A potential key factor in plant-pathogen interactions is fungal sesquiterpenoids that have myco- and phytotoxic activities. The first committed step in sesquiterpenoids biosynthesised is performed by sesquiterpene synthases (TPS). Only a few TPS's have been functionally characterized from filamentous fungi and none from the Colletotrichum genus. Despite it as an important fungal pathogen to agriculture, it is poorly understood at the molecular and chemical level. The terpenoid biochemistry in C. acutatum strain SA 0-1 was studied and one C. acutatum TPS (CaTPS) was successfully cloned and characterized in yeast. CaTPS catalyses the biosynthesis of multiple sesquiterpenoids. The two major products are -caryophyllene and an unidentified sesquiterpenoid along with -humulene as one the minor sesquiterpenoid products. These products were also secreted by the fungus in strawberry fruit media along with several other sesquiterpenoids indicating other TPS's are active during in vitro growth. -Caryophyllene and -humulene are known cytotoxic products important for ecological interactions and are produced by SA 0-1. Interestingly, a gene expression analysis using qRT-PCR revealed a significant increase in expression of CaTPS during strawberry fruit infection thus indicating that it could be involved in fruit infection. This is the first characterization of TPS in Colletotrichum spp. and terpenoid profiles of C. acutatum, which could facilitate studies on the role of terpenoids in the ecology of C. acutatum.",
author = "Amby, {Daniel Buchvaldt} and Tom Manczak and Petersen, {Mikael Agerlin} and Thomas Sundelin and Corinna Weitzel and Maciej Grajewski and Simonsen, {Henrik Toft} and Birgit Jensen",
year = "2016",
doi = "10.1099/mic.0.000361",
language = "English",
volume = "162",
pages = "1773--1783",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - Role of the Colletotrichum acutatum sesquiterpene synthase CaTPS in the biosynthesis of sesquiterpenoids

AU - Amby, Daniel Buchvaldt

AU - Manczak, Tom

AU - Petersen, Mikael Agerlin

AU - Sundelin, Thomas

AU - Weitzel, Corinna

AU - Grajewski, Maciej

AU - Simonsen, Henrik Toft

AU - Jensen, Birgit

PY - 2016

Y1 - 2016

N2 - Colletotrichum acutatum is a major fungal pathogen of fruit crops, which causes severe yield losses in strawberry productions. A potential key factor in plant-pathogen interactions is fungal sesquiterpenoids that have myco- and phytotoxic activities. The first committed step in sesquiterpenoids biosynthesised is performed by sesquiterpene synthases (TPS). Only a few TPS's have been functionally characterized from filamentous fungi and none from the Colletotrichum genus. Despite it as an important fungal pathogen to agriculture, it is poorly understood at the molecular and chemical level. The terpenoid biochemistry in C. acutatum strain SA 0-1 was studied and one C. acutatum TPS (CaTPS) was successfully cloned and characterized in yeast. CaTPS catalyses the biosynthesis of multiple sesquiterpenoids. The two major products are -caryophyllene and an unidentified sesquiterpenoid along with -humulene as one the minor sesquiterpenoid products. These products were also secreted by the fungus in strawberry fruit media along with several other sesquiterpenoids indicating other TPS's are active during in vitro growth. -Caryophyllene and -humulene are known cytotoxic products important for ecological interactions and are produced by SA 0-1. Interestingly, a gene expression analysis using qRT-PCR revealed a significant increase in expression of CaTPS during strawberry fruit infection thus indicating that it could be involved in fruit infection. This is the first characterization of TPS in Colletotrichum spp. and terpenoid profiles of C. acutatum, which could facilitate studies on the role of terpenoids in the ecology of C. acutatum.

AB - Colletotrichum acutatum is a major fungal pathogen of fruit crops, which causes severe yield losses in strawberry productions. A potential key factor in plant-pathogen interactions is fungal sesquiterpenoids that have myco- and phytotoxic activities. The first committed step in sesquiterpenoids biosynthesised is performed by sesquiterpene synthases (TPS). Only a few TPS's have been functionally characterized from filamentous fungi and none from the Colletotrichum genus. Despite it as an important fungal pathogen to agriculture, it is poorly understood at the molecular and chemical level. The terpenoid biochemistry in C. acutatum strain SA 0-1 was studied and one C. acutatum TPS (CaTPS) was successfully cloned and characterized in yeast. CaTPS catalyses the biosynthesis of multiple sesquiterpenoids. The two major products are -caryophyllene and an unidentified sesquiterpenoid along with -humulene as one the minor sesquiterpenoid products. These products were also secreted by the fungus in strawberry fruit media along with several other sesquiterpenoids indicating other TPS's are active during in vitro growth. -Caryophyllene and -humulene are known cytotoxic products important for ecological interactions and are produced by SA 0-1. Interestingly, a gene expression analysis using qRT-PCR revealed a significant increase in expression of CaTPS during strawberry fruit infection thus indicating that it could be involved in fruit infection. This is the first characterization of TPS in Colletotrichum spp. and terpenoid profiles of C. acutatum, which could facilitate studies on the role of terpenoids in the ecology of C. acutatum.

U2 - 10.1099/mic.0.000361

DO - 10.1099/mic.0.000361

M3 - Journal article

C2 - 27553953

VL - 162

SP - 1773

EP - 1783

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 10

ER -

ID: 166321442