Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens

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Standard

Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens. / van der Graaff, Eric; Hooykaas, P J.

In: Plant Cell Reports, Vol. 15, No. 8, 04.1996, p. 572-7.

Research output: Contribution to journal › Journal article › Research

Harvard

van der Graaff, E & Hooykaas, PJ 1996, 'Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens', Plant Cell Reports, vol. 15, no. 8, pp. 572-7. https://doi.org/10.1007/BF00232455

APA

van der Graaff, E., & Hooykaas, P. J. (1996). Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens. Plant Cell Reports, 15(8), 572-7. https://doi.org/10.1007/BF00232455

Vancouver

van der Graaff E, Hooykaas PJ. Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens. Plant Cell Reports. 1996 Apr;15(8):572-7. https://doi.org/10.1007/BF00232455

Author

van der Graaff, Eric ; Hooykaas, P J. / Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens. In: Plant Cell Reports. 1996 ; Vol. 15, No. 8. pp. 572-7.

Bibtex

@article{44107f5b3e774d6da69bee40ec808074,
title = "Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens",
abstract = "We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces seeds with an average seed yield of 100-300 seeds per plant. This improved transformation protocol yields mainly (70%) transformants segregating for a single T-DNA locus of which 68% actually contain one T-DNA insert. The objective is to generate a pool of independent transformants harboring an activator T-DNA construct in a gene tagging approach to isolate genes involved in morphogenesis and auxin signal transduction.",
author = "{van der Graaff}, Eric and Hooykaas, {P J}",
year = "1996",
month = apr,
doi = "10.1007/BF00232455",
language = "English",
volume = "15",
pages = "572--7",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer",
number = "8",

}

RIS

TY - JOUR

T1 - Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens

AU - van der Graaff, Eric

AU - Hooykaas, P J

PY - 1996/4

Y1 - 1996/4

N2 - We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces seeds with an average seed yield of 100-300 seeds per plant. This improved transformation protocol yields mainly (70%) transformants segregating for a single T-DNA locus of which 68% actually contain one T-DNA insert. The objective is to generate a pool of independent transformants harboring an activator T-DNA construct in a gene tagging approach to isolate genes involved in morphogenesis and auxin signal transduction.

AB - We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces seeds with an average seed yield of 100-300 seeds per plant. This improved transformation protocol yields mainly (70%) transformants segregating for a single T-DNA locus of which 68% actually contain one T-DNA insert. The objective is to generate a pool of independent transformants harboring an activator T-DNA construct in a gene tagging approach to isolate genes involved in morphogenesis and auxin signal transduction.

U2 - 10.1007/BF00232455

DO - 10.1007/BF00232455

M3 - Journal article

C2 - 24178520

VL - 15

SP - 572

EP - 577

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 8

ER -

ID: 106392006