Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures

Research output: Contribution to journalJournal articlepeer-review

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Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures. / Barba-Espín, Gregorio; Chen, Shih-Ti; Agnolet, Sara; Nymark Hegelund, Josefine; Stanstrup, Jan; Christensen, Jan H; Müller, Renate; Lütken, Henrik.

In: Journal of Experimental Botany, Vol. 71, No. 22, 2020, p. 7030-7045.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Barba-Espín, G, Chen, S-T, Agnolet, S, Nymark Hegelund, J, Stanstrup, J, Christensen, JH, Müller, R & Lütken, H 2020, 'Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures', Journal of Experimental Botany, vol. 71, no. 22, pp. 7030-7045. https://doi.org/10.1093/jxb/eraa376

APA

Barba-Espín, G., Chen, S-T., Agnolet, S., Nymark Hegelund, J., Stanstrup, J., Christensen, J. H., Müller, R., & Lütken, H. (2020). Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures. Journal of Experimental Botany, 71(22), 7030-7045. https://doi.org/10.1093/jxb/eraa376

Vancouver

Barba-Espín G, Chen S-T, Agnolet S, Nymark Hegelund J, Stanstrup J, Christensen JH et al. Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures. Journal of Experimental Botany. 2020;71(22):7030-7045. https://doi.org/10.1093/jxb/eraa376

Author

Barba-Espín, Gregorio ; Chen, Shih-Ti ; Agnolet, Sara ; Nymark Hegelund, Josefine ; Stanstrup, Jan ; Christensen, Jan H ; Müller, Renate ; Lütken, Henrik. / Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures. In: Journal of Experimental Botany. 2020 ; Vol. 71, No. 22. pp. 7030-7045.

Bibtex

@article{7bd91e460a2a4be49e664bfa319de6f4,
title = "Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures",
abstract = "Hairy root (HR) cultures are quickly evolving as fundamental research tool and as bio-based production system for secondary metabolites. In this study, an efficient protocol for establishment and elicitation of anthocyanin-producing HR cultures from black carrot was established. Taproot and hypocotyl explants of four carrot cultivars were transformed using wild-type Rhizobium rhizogenes. HR growth performance on plates was monitored to identify three fast-growing HR lines, two originating from root explants (lines NB-R and 43-R) and one from a hypocotyl explant (line 43-H). The HR biomass accumulated 25- to 30-fold in liquid media over a 4-week period. Nine anthocyanins and 19 hydroxycinnamic acid derivatives were identified and monitored using UPLC-PDA-TOF during HR growth. Adding ethephon, an ethylene-releasing compound, to the HR culture substantially increased the anthocyanin content by up to 82% in line 43-R and hydroxycinnamic acid concentrations by over 20% in line NB-R. Moreover, the activities of superoxide dismutase and glutathione-S-transferase increased in the HRs in response to ethephon, which could be related to the functionality and compartmentalisation of anthocyanins. These findings present black carrot HR cultures as a platform for the in vitro production of anthocyanins and antioxidants and add insight into the regulation of secondary metabolism in black carrot.",
author = "Gregorio Barba-Esp{\'i}n and Shih-Ti Chen and Sara Agnolet and {Nymark Hegelund}, Josefine and Jan Stanstrup and Christensen, {Jan H} and Renate M{\"u}ller and Henrik L{\"u}tken",
year = "2020",
doi = "10.1093/jxb/eraa376",
language = "English",
volume = "71",
pages = "7030--7045",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "22",

}

RIS

TY - JOUR

T1 - Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures

AU - Barba-Espín, Gregorio

AU - Chen, Shih-Ti

AU - Agnolet, Sara

AU - Nymark Hegelund, Josefine

AU - Stanstrup, Jan

AU - Christensen, Jan H

AU - Müller, Renate

AU - Lütken, Henrik

PY - 2020

Y1 - 2020

N2 - Hairy root (HR) cultures are quickly evolving as fundamental research tool and as bio-based production system for secondary metabolites. In this study, an efficient protocol for establishment and elicitation of anthocyanin-producing HR cultures from black carrot was established. Taproot and hypocotyl explants of four carrot cultivars were transformed using wild-type Rhizobium rhizogenes. HR growth performance on plates was monitored to identify three fast-growing HR lines, two originating from root explants (lines NB-R and 43-R) and one from a hypocotyl explant (line 43-H). The HR biomass accumulated 25- to 30-fold in liquid media over a 4-week period. Nine anthocyanins and 19 hydroxycinnamic acid derivatives were identified and monitored using UPLC-PDA-TOF during HR growth. Adding ethephon, an ethylene-releasing compound, to the HR culture substantially increased the anthocyanin content by up to 82% in line 43-R and hydroxycinnamic acid concentrations by over 20% in line NB-R. Moreover, the activities of superoxide dismutase and glutathione-S-transferase increased in the HRs in response to ethephon, which could be related to the functionality and compartmentalisation of anthocyanins. These findings present black carrot HR cultures as a platform for the in vitro production of anthocyanins and antioxidants and add insight into the regulation of secondary metabolism in black carrot.

AB - Hairy root (HR) cultures are quickly evolving as fundamental research tool and as bio-based production system for secondary metabolites. In this study, an efficient protocol for establishment and elicitation of anthocyanin-producing HR cultures from black carrot was established. Taproot and hypocotyl explants of four carrot cultivars were transformed using wild-type Rhizobium rhizogenes. HR growth performance on plates was monitored to identify three fast-growing HR lines, two originating from root explants (lines NB-R and 43-R) and one from a hypocotyl explant (line 43-H). The HR biomass accumulated 25- to 30-fold in liquid media over a 4-week period. Nine anthocyanins and 19 hydroxycinnamic acid derivatives were identified and monitored using UPLC-PDA-TOF during HR growth. Adding ethephon, an ethylene-releasing compound, to the HR culture substantially increased the anthocyanin content by up to 82% in line 43-R and hydroxycinnamic acid concentrations by over 20% in line NB-R. Moreover, the activities of superoxide dismutase and glutathione-S-transferase increased in the HRs in response to ethephon, which could be related to the functionality and compartmentalisation of anthocyanins. These findings present black carrot HR cultures as a platform for the in vitro production of anthocyanins and antioxidants and add insight into the regulation of secondary metabolism in black carrot.

U2 - 10.1093/jxb/eraa376

DO - 10.1093/jxb/eraa376

M3 - Journal article

C2 - 32803264

VL - 71

SP - 7030

EP - 7045

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 22

ER -

ID: 248725877