Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures
Research output: Contribution to journal › Journal article › peer-review
Standard
Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures. / Barba-Espín, Gregorio; Chen, Shih-Ti; Agnolet, Sara; Nymark Hegelund, Josefine; Stanstrup, Jan; Christensen, Jan H; Müller, Renate; Lütken, Henrik.
In: Journal of Experimental Botany, Vol. 71, No. 22, 2020, p. 7030-7045.Research output: Contribution to journal › Journal article › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures
AU - Barba-Espín, Gregorio
AU - Chen, Shih-Ti
AU - Agnolet, Sara
AU - Nymark Hegelund, Josefine
AU - Stanstrup, Jan
AU - Christensen, Jan H
AU - Müller, Renate
AU - Lütken, Henrik
PY - 2020
Y1 - 2020
N2 - Hairy root (HR) cultures are quickly evolving as fundamental research tool and as bio-based production system for secondary metabolites. In this study, an efficient protocol for establishment and elicitation of anthocyanin-producing HR cultures from black carrot was established. Taproot and hypocotyl explants of four carrot cultivars were transformed using wild-type Rhizobium rhizogenes. HR growth performance on plates was monitored to identify three fast-growing HR lines, two originating from root explants (lines NB-R and 43-R) and one from a hypocotyl explant (line 43-H). The HR biomass accumulated 25- to 30-fold in liquid media over a 4-week period. Nine anthocyanins and 19 hydroxycinnamic acid derivatives were identified and monitored using UPLC-PDA-TOF during HR growth. Adding ethephon, an ethylene-releasing compound, to the HR culture substantially increased the anthocyanin content by up to 82% in line 43-R and hydroxycinnamic acid concentrations by over 20% in line NB-R. Moreover, the activities of superoxide dismutase and glutathione-S-transferase increased in the HRs in response to ethephon, which could be related to the functionality and compartmentalisation of anthocyanins. These findings present black carrot HR cultures as a platform for the in vitro production of anthocyanins and antioxidants and add insight into the regulation of secondary metabolism in black carrot.
AB - Hairy root (HR) cultures are quickly evolving as fundamental research tool and as bio-based production system for secondary metabolites. In this study, an efficient protocol for establishment and elicitation of anthocyanin-producing HR cultures from black carrot was established. Taproot and hypocotyl explants of four carrot cultivars were transformed using wild-type Rhizobium rhizogenes. HR growth performance on plates was monitored to identify three fast-growing HR lines, two originating from root explants (lines NB-R and 43-R) and one from a hypocotyl explant (line 43-H). The HR biomass accumulated 25- to 30-fold in liquid media over a 4-week period. Nine anthocyanins and 19 hydroxycinnamic acid derivatives were identified and monitored using UPLC-PDA-TOF during HR growth. Adding ethephon, an ethylene-releasing compound, to the HR culture substantially increased the anthocyanin content by up to 82% in line 43-R and hydroxycinnamic acid concentrations by over 20% in line NB-R. Moreover, the activities of superoxide dismutase and glutathione-S-transferase increased in the HRs in response to ethephon, which could be related to the functionality and compartmentalisation of anthocyanins. These findings present black carrot HR cultures as a platform for the in vitro production of anthocyanins and antioxidants and add insight into the regulation of secondary metabolism in black carrot.
U2 - 10.1093/jxb/eraa376
DO - 10.1093/jxb/eraa376
M3 - Journal article
C2 - 32803264
VL - 71
SP - 7030
EP - 7045
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
SN - 0022-0957
IS - 22
ER -
ID: 248725877