Biochar has shown beneficial effects in agricultural production, yet the combined effects of biochar and reduced irrigation on crop growth and water-use efficiency (WUE) in diverse soil types have not been fully explored. A split-root pot experiment was conducted to investigate the effects of addition of 2% softwood (SWB) and wheat straw biochar (WSB) on growth, physiology, WUE and nutrients uptake of tobacco (Nicotiana tabacum L.) plants grown in a Ferrosol and an Anthrosol, respectively, under three irrigation treatments. The plants were either irrigated daily to 90% of water-holding capacity (FI), or irrigated with 70% volume of water used for FI to the whole root-zone (DI) or alternately to half root-zone (PRD). The results showed that plants grown in Anthrosol possessed greater leaf gas exchange rates, dry biomass and WUE while lower nutrients content compared to those grown in Ferrosol. Despite a negative effect on plant N content and WUE, WSB addition increased water-holding capacity, consequently improved leaf gas exchange, water uptake, biomass and K content resulting in an improved in the leaf quality of tobacco as exemplified by an increased leaf K content and a more appropriate N to K stoichiometric ratio. However, these effects were not evident upon SWB addition. Moreover, these responses to biochar addition were stronger in Ferrosol than in Anthrosol might be associated with its lower pH. Compared to FI, PRD slightly reduced photosynthetic rate but significantly decreased stomatal conductance, transpiration rate and leaf area, leading to a significant increase in intrinsic, instantaneous and plant WUE. Additionally, PRD was superior over DI in improving yield, WUE, N uptake under a same irrigation volume. It was concluded that WSB combined with PRD could be a promising practice to synergistically improve tobacco yield, quality and WUE by improving soil hydro-physical properties and nutrients bioavailability.