DNA barcoding and isolation of vertically transmitted ascomycetes in sorghum from Burkina Faso: Epicoccum sorghinum is dominant in seedlings and appears as a common root pathogen

Research output: Contribution to journalJournal articlepeer-review

Standard

DNA barcoding and isolation of vertically transmitted ascomycetes in sorghum from Burkina Faso : Epicoccum sorghinum is dominant in seedlings and appears as a common root pathogen. / Stokholm, Michaela Schiller; Wulff, Ednar G.; Zida, Elisabeth P.; Thio, Ibié G.; Néya, James B.; Soalla, Romain W.; Glazowska, Sylwia Emilia; Andresen, Marianne; Topbjerg, Henrik Bak; Boelt, Birte; Lund, Ole Søgaard.

In: Microbiological Research, Vol. 191, 2016, p. 38-50.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Stokholm, MS, Wulff, EG, Zida, EP, Thio, IG, Néya, JB, Soalla, RW, Glazowska, SE, Andresen, M, Topbjerg, HB, Boelt, B & Lund, OS 2016, 'DNA barcoding and isolation of vertically transmitted ascomycetes in sorghum from Burkina Faso: Epicoccum sorghinum is dominant in seedlings and appears as a common root pathogen', Microbiological Research, vol. 191, pp. 38-50. https://doi.org/10.1016/j.micres.2016.05.004

APA

Stokholm, M. S., Wulff, E. G., Zida, E. P., Thio, I. G., Néya, J. B., Soalla, R. W., Glazowska, S. E., Andresen, M., Topbjerg, H. B., Boelt, B., & Lund, O. S. (2016). DNA barcoding and isolation of vertically transmitted ascomycetes in sorghum from Burkina Faso: Epicoccum sorghinum is dominant in seedlings and appears as a common root pathogen. Microbiological Research, 191, 38-50. https://doi.org/10.1016/j.micres.2016.05.004

Vancouver

Stokholm MS, Wulff EG, Zida EP, Thio IG, Néya JB, Soalla RW et al. DNA barcoding and isolation of vertically transmitted ascomycetes in sorghum from Burkina Faso: Epicoccum sorghinum is dominant in seedlings and appears as a common root pathogen. Microbiological Research. 2016;191:38-50. https://doi.org/10.1016/j.micres.2016.05.004

Author

Stokholm, Michaela Schiller ; Wulff, Ednar G. ; Zida, Elisabeth P. ; Thio, Ibié G. ; Néya, James B. ; Soalla, Romain W. ; Glazowska, Sylwia Emilia ; Andresen, Marianne ; Topbjerg, Henrik Bak ; Boelt, Birte ; Lund, Ole Søgaard. / DNA barcoding and isolation of vertically transmitted ascomycetes in sorghum from Burkina Faso : Epicoccum sorghinum is dominant in seedlings and appears as a common root pathogen. In: Microbiological Research. 2016 ; Vol. 191. pp. 38-50.

Bibtex

@article{962a2b6aa0974f70a83e27b3f2e261d0,
title = "DNA barcoding and isolation of vertically transmitted ascomycetes in sorghum from Burkina Faso: Epicoccum sorghinum is dominant in seedlings and appears as a common root pathogen",
abstract = "Molecular identification of fungal taxa commonly transmitted through seeds of sorghum in Western Africa is lacking. In the present study, farm-saved seeds, collected from four villages in Northern Burkina Faso, were surface sterilized and the distribution of fungal DNA in seeds and seven-day-old seedlings was analyzed by 18S ribosomal DNA (rDNA) amplicon sequencing. More than 99% of the fungal rDNA was found to originate from ascomycetes. The distribution of ascomycetes at species level was subsequently analyzed by barcoding of ITS2 rDNA. Eighteen Operational Taxonomic Units (OTUs) were identified from seedlings, compared to 29 OTUs from seeds. The top-eight most abundant ascomycete OTUs from seedlings were annotated as: Epicoccum sorghinum, Fusarium thapsinum, four different Curvularia spp., Exserohilum rostratum and Alternaria longissima. These OTUs were also present in amplicons from seed samples collected in Central Burkina Faso confirming a common occurrence. E. sorghinum was highly predominant in seedlings both measured by DNA analysis and by isolation. The dominance of E. sorghinum was particularly strong in roots from poorly growing seedlings. Pathogenicity of E. sorghinum isolates was compared to F. thapsinum by inoculation to seeds in vitro. Both fungal species caused significant inhibition of seedling growth (P<0.001) and Koch's postulates were fulfilled. Extensive, dark necrosis in roots was a typical symptom of E. sorghinum, whereas wilting of leaves was caused primarily by F. thapsinum. This study provides the first molecular approach to characterize the seedling mycoflora of sorghum in Western Africa and suggests E. sorghinum as a common root pathogen.",
keywords = "Journal Article",
author = "Stokholm, {Michaela Schiller} and Wulff, {Ednar G.} and Zida, {Elisabeth P.} and Thio, {Ibi{\'e} G.} and N{\'e}ya, {James B.} and Soalla, {Romain W.} and Glazowska, {Sylwia Emilia} and Marianne Andresen and Topbjerg, {Henrik Bak} and Birte Boelt and Lund, {Ole S{\o}gaard}",
note = "Copyright {\textcopyright} 2016 The Authors. Published by Elsevier GmbH.. All rights reserved.",
year = "2016",
doi = "10.1016/j.micres.2016.05.004",
language = "English",
volume = "191",
pages = "38--50",
journal = "Microbiological Research",
issn = "0944-5013",
publisher = "Elsevier GmbH - Urban und Fischer",

}

RIS

TY - JOUR

T1 - DNA barcoding and isolation of vertically transmitted ascomycetes in sorghum from Burkina Faso

T2 - Epicoccum sorghinum is dominant in seedlings and appears as a common root pathogen

AU - Stokholm, Michaela Schiller

AU - Wulff, Ednar G.

AU - Zida, Elisabeth P.

AU - Thio, Ibié G.

AU - Néya, James B.

AU - Soalla, Romain W.

AU - Glazowska, Sylwia Emilia

AU - Andresen, Marianne

AU - Topbjerg, Henrik Bak

AU - Boelt, Birte

AU - Lund, Ole Søgaard

N1 - Copyright © 2016 The Authors. Published by Elsevier GmbH.. All rights reserved.

PY - 2016

Y1 - 2016

N2 - Molecular identification of fungal taxa commonly transmitted through seeds of sorghum in Western Africa is lacking. In the present study, farm-saved seeds, collected from four villages in Northern Burkina Faso, were surface sterilized and the distribution of fungal DNA in seeds and seven-day-old seedlings was analyzed by 18S ribosomal DNA (rDNA) amplicon sequencing. More than 99% of the fungal rDNA was found to originate from ascomycetes. The distribution of ascomycetes at species level was subsequently analyzed by barcoding of ITS2 rDNA. Eighteen Operational Taxonomic Units (OTUs) were identified from seedlings, compared to 29 OTUs from seeds. The top-eight most abundant ascomycete OTUs from seedlings were annotated as: Epicoccum sorghinum, Fusarium thapsinum, four different Curvularia spp., Exserohilum rostratum and Alternaria longissima. These OTUs were also present in amplicons from seed samples collected in Central Burkina Faso confirming a common occurrence. E. sorghinum was highly predominant in seedlings both measured by DNA analysis and by isolation. The dominance of E. sorghinum was particularly strong in roots from poorly growing seedlings. Pathogenicity of E. sorghinum isolates was compared to F. thapsinum by inoculation to seeds in vitro. Both fungal species caused significant inhibition of seedling growth (P<0.001) and Koch's postulates were fulfilled. Extensive, dark necrosis in roots was a typical symptom of E. sorghinum, whereas wilting of leaves was caused primarily by F. thapsinum. This study provides the first molecular approach to characterize the seedling mycoflora of sorghum in Western Africa and suggests E. sorghinum as a common root pathogen.

AB - Molecular identification of fungal taxa commonly transmitted through seeds of sorghum in Western Africa is lacking. In the present study, farm-saved seeds, collected from four villages in Northern Burkina Faso, were surface sterilized and the distribution of fungal DNA in seeds and seven-day-old seedlings was analyzed by 18S ribosomal DNA (rDNA) amplicon sequencing. More than 99% of the fungal rDNA was found to originate from ascomycetes. The distribution of ascomycetes at species level was subsequently analyzed by barcoding of ITS2 rDNA. Eighteen Operational Taxonomic Units (OTUs) were identified from seedlings, compared to 29 OTUs from seeds. The top-eight most abundant ascomycete OTUs from seedlings were annotated as: Epicoccum sorghinum, Fusarium thapsinum, four different Curvularia spp., Exserohilum rostratum and Alternaria longissima. These OTUs were also present in amplicons from seed samples collected in Central Burkina Faso confirming a common occurrence. E. sorghinum was highly predominant in seedlings both measured by DNA analysis and by isolation. The dominance of E. sorghinum was particularly strong in roots from poorly growing seedlings. Pathogenicity of E. sorghinum isolates was compared to F. thapsinum by inoculation to seeds in vitro. Both fungal species caused significant inhibition of seedling growth (P<0.001) and Koch's postulates were fulfilled. Extensive, dark necrosis in roots was a typical symptom of E. sorghinum, whereas wilting of leaves was caused primarily by F. thapsinum. This study provides the first molecular approach to characterize the seedling mycoflora of sorghum in Western Africa and suggests E. sorghinum as a common root pathogen.

KW - Journal Article

U2 - 10.1016/j.micres.2016.05.004

DO - 10.1016/j.micres.2016.05.004

M3 - Journal article

C2 - 27524652

VL - 191

SP - 38

EP - 50

JO - Microbiological Research

JF - Microbiological Research

SN - 0944-5013

ER -

ID: 169106731