Deviating T-DNA transfer from Agrobacterium tumefaciens to plants

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Deviating T-DNA transfer from Agrobacterium tumefaciens to plants. / van der Graaff, Eric; den Dulk-Ras, A; Hooykaas, P J.

In: Plant Molecular Biology, Vol. 31, No. 3, 06.1996, p. 677-81.

Research output: Contribution to journal › Journal article › Research

Harvard

van der Graaff, E, den Dulk-Ras, A & Hooykaas, PJ 1996, 'Deviating T-DNA transfer from Agrobacterium tumefaciens to plants', Plant Molecular Biology, vol. 31, no. 3, pp. 677-81.

APA

van der Graaff, E., den Dulk-Ras, A., & Hooykaas, P. J. (1996). Deviating T-DNA transfer from Agrobacterium tumefaciens to plants. Plant Molecular Biology, 31(3), 677-81.

Vancouver

van der Graaff E, den Dulk-Ras A, Hooykaas PJ. Deviating T-DNA transfer from Agrobacterium tumefaciens to plants. Plant Molecular Biology. 1996 Jun;31(3):677-81.

Author

van der Graaff, Eric ; den Dulk-Ras, A ; Hooykaas, P J. / Deviating T-DNA transfer from Agrobacterium tumefaciens to plants. In: Plant Molecular Biology. 1996 ; Vol. 31, No. 3. pp. 677-81.

Bibtex

@article{9ec9a2e228ae497a9c6cad2a22684cd7,
title = "Deviating T-DNA transfer from Agrobacterium tumefaciens to plants",
abstract = "We analyzed 29 T-DNA inserts in transgenic Arabidopsis thaliana plants for the junction of the right border sequences and the flanking plant DNA. DNA sequencing showed that in most lines the right border sequences transferred had been preserved during integration, corroborating literature data. Surprisingly, in four independent transgenic lines a complete right border repeat was present followed by binary vector sequences. Cloning of two of these T-DNA inserts by plasmid rescue showed that in these lines the transferred DNA consisted of the complete binary vector sequences in addition to the T-region. On the basis of the structure of the transferred DNA we propose that in these lines T-DNA transfer started at the left-border repeat, continued through the vector part, passed the right border repeat, and ended only after reaching again this left-border repeat.",
keywords = "Agrobacterium tumefaciens, Arabidopsis, Base Sequence, DNA Primers, DNA, Bacterial, DNA, Plant, DNA, Single-Stranded, Genetic Vectors, Molecular Sequence Data, Plants, Genetically Modified, Plasmids, Polymerase Chain Reaction, Transfection",
author = "{van der Graaff}, Eric and {den Dulk-Ras}, A and Hooykaas, {P J}",
year = "1996",
month = jun,
language = "English",
volume = "31",
pages = "677--81",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - Deviating T-DNA transfer from Agrobacterium tumefaciens to plants

AU - van der Graaff, Eric

AU - den Dulk-Ras, A

AU - Hooykaas, P J

PY - 1996/6

Y1 - 1996/6

N2 - We analyzed 29 T-DNA inserts in transgenic Arabidopsis thaliana plants for the junction of the right border sequences and the flanking plant DNA. DNA sequencing showed that in most lines the right border sequences transferred had been preserved during integration, corroborating literature data. Surprisingly, in four independent transgenic lines a complete right border repeat was present followed by binary vector sequences. Cloning of two of these T-DNA inserts by plasmid rescue showed that in these lines the transferred DNA consisted of the complete binary vector sequences in addition to the T-region. On the basis of the structure of the transferred DNA we propose that in these lines T-DNA transfer started at the left-border repeat, continued through the vector part, passed the right border repeat, and ended only after reaching again this left-border repeat.

AB - We analyzed 29 T-DNA inserts in transgenic Arabidopsis thaliana plants for the junction of the right border sequences and the flanking plant DNA. DNA sequencing showed that in most lines the right border sequences transferred had been preserved during integration, corroborating literature data. Surprisingly, in four independent transgenic lines a complete right border repeat was present followed by binary vector sequences. Cloning of two of these T-DNA inserts by plasmid rescue showed that in these lines the transferred DNA consisted of the complete binary vector sequences in addition to the T-region. On the basis of the structure of the transferred DNA we propose that in these lines T-DNA transfer started at the left-border repeat, continued through the vector part, passed the right border repeat, and ended only after reaching again this left-border repeat.

KW - Agrobacterium tumefaciens

KW - Arabidopsis

KW - Base Sequence

KW - DNA Primers

KW - DNA, Bacterial

KW - DNA, Plant

KW - DNA, Single-Stranded

KW - Genetic Vectors

KW - Molecular Sequence Data

KW - Plants, Genetically Modified

KW - Plasmids

KW - Polymerase Chain Reaction

KW - Transfection

M3 - Journal article

C2 - 8790299

VL - 31

SP - 677

EP - 681

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 3

ER -

ID: 106391986