Overexpression of the AtSHI gene in poinsettia, Euphorbia pulcherrima, results in compact plants
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Overexpression of the AtSHI gene in poinsettia, Euphorbia pulcherrima, results in compact plants. / Islam, M. Ashraful; Lütken, Henrik Vlk; Haugslien, Sissel ; Blystad, Dag-Ragner; Torre, Sissel ; Rolcik, Jakub; Rasmussen, Søren Kjærsgaard; Olsen, Jorunn E.; Clarke, Jihong Liu .
In: P L o S One, Vol. 8, No. 1, e53377, 2013.Research output: Contribution to journal › Journal article › peer-review
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T1 - Overexpression of the AtSHI gene in poinsettia, Euphorbia pulcherrima, results in compact plants
AU - Islam, M. Ashraful
AU - Lütken, Henrik Vlk
AU - Haugslien, Sissel
AU - Blystad, Dag-Ragner
AU - Torre, Sissel
AU - Rolcik, Jakub
AU - Rasmussen, Søren Kjærsgaard
AU - Olsen, Jorunn E.
AU - Clarke, Jihong Liu
PY - 2013
Y1 - 2013
N2 - Euphorbia pulcherrima, poinsettia, is a non-food and non-feed vegetatively propagated ornamental plant. Appropriate plant height is one of the most important traits in poinsettia production and is commonly achieved by application of chemical growth retardants. To produce compact poinsettia plants with desirable height and reduce the utilization of growth retardants, the Arabidopsis SHORT INTERNODE (AtSHI) gene controlled by the cauliflower mosaic virus 35S promoter was introduced into poinsettia by Agrobacterium-mediated transformation. Three independent transgenic lines were produced and stable integration of transgene was verified by PCR and Southern blot analysis. Reduced plant height (21–52%) and internode lengths (31–49%) were obtained in the transgenic lines compared to control plants. This correlates positively with the AtSHI transcript levels, with the highest levels in the most dwarfed transgenic line (TL1). The indole-3-acetic acid (IAA) content appeared lower (11–31% reduction) in the transgenic lines compared to the wild type (WT) controls, with the lowest level (31% reduction) in TL1. Total internode numbers, bract numbers and bract area were significantly reduced in all transgenic lines in comparison with the WT controls. Only TL1 showed significantly lower plant diameter, total leaf area and total dry weight, whereas none of the AtSHI expressing lines showed altered timing of flower initiation, cyathia abscission or bract necrosis. This study demonstrated that introduction of the AtSHI gene into poinsettia by genetic engineering can be an effective approach in controlling plant height without negatively affecting flowering time. This can help to reduce or avoid the use of toxic growth retardants of environmental and human health concern. This is the first report that AtSHI gene was overexpressed in poinsettia and transgenic poinsettia plants with compact growth were produced
AB - Euphorbia pulcherrima, poinsettia, is a non-food and non-feed vegetatively propagated ornamental plant. Appropriate plant height is one of the most important traits in poinsettia production and is commonly achieved by application of chemical growth retardants. To produce compact poinsettia plants with desirable height and reduce the utilization of growth retardants, the Arabidopsis SHORT INTERNODE (AtSHI) gene controlled by the cauliflower mosaic virus 35S promoter was introduced into poinsettia by Agrobacterium-mediated transformation. Three independent transgenic lines were produced and stable integration of transgene was verified by PCR and Southern blot analysis. Reduced plant height (21–52%) and internode lengths (31–49%) were obtained in the transgenic lines compared to control plants. This correlates positively with the AtSHI transcript levels, with the highest levels in the most dwarfed transgenic line (TL1). The indole-3-acetic acid (IAA) content appeared lower (11–31% reduction) in the transgenic lines compared to the wild type (WT) controls, with the lowest level (31% reduction) in TL1. Total internode numbers, bract numbers and bract area were significantly reduced in all transgenic lines in comparison with the WT controls. Only TL1 showed significantly lower plant diameter, total leaf area and total dry weight, whereas none of the AtSHI expressing lines showed altered timing of flower initiation, cyathia abscission or bract necrosis. This study demonstrated that introduction of the AtSHI gene into poinsettia by genetic engineering can be an effective approach in controlling plant height without negatively affecting flowering time. This can help to reduce or avoid the use of toxic growth retardants of environmental and human health concern. This is the first report that AtSHI gene was overexpressed in poinsettia and transgenic poinsettia plants with compact growth were produced
U2 - 10.1371/journal.pone.0053377
DO - 10.1371/journal.pone.0053377
M3 - Journal article
C2 - 23308204
VL - 8
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 1
M1 - e53377
ER -
ID: 41927391