Signal transduction – University of Copenhagen

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Plant and Environmental Sciences > Research > Transport Biology > Signal transduction

Signal transduction

In our group we identify components of the signal transduction pathways leading to regulation of the H+ pump.

The plasma membrane H+-pump is an essential enzyme being involved in developmental processes as well as responding to external physiological stimuli. This requires a tight coordination of protein kinases and phosphatases regulating the activity of the pump by phosphorylation events.

A wide range of techniques are employed in order to fully understand the processes taking place at the cellular level in the plant: molecular biology, generation and characterization of mutants, heterologous expression of proteins, protein-protein interaction, enzyme activity, bioimaging and proteomics.

 

Selected publications:

  • Rudashevskaya EL, Ye J, Jensen ON, Fuglsang AT, Palmgren MG (2012) Phosphosite Mapping of P-type Plasma Membrane H+-ATPase in Homologous and Heterologous Environments. J. Biol. Chem. 287:4904 - 4913.
  • Du W, Lin H, Chen S, Wu Y, Zhang J, Fuglsang AT, Palmgren MG, Wu W, Guo Y (2011) Phosphorylation of SOS3-Like Calcium-Binding Proteins by Their Interacting SOS2-Like Protein Kinases Is a Common Regulatory Mechanism in Arabidopsis. Plant Physiology 156:2235-2243
  • Yang Y, Qin Y, Xie C, Zhao F, Zhao J, Liu D et al (2010). The Arabidopsis chaperone J3 regulates the plasma membrane H+-ATPase through interaction with the PKS5 Kinase. Plant Cell 22(4):1313-1332 
  • Liu J, Elmore JM, Fuglsang AT , Palmgren M, Staskawicz BJ, Coaker G (2009). RIN4 functions with plasma membrane H+-ATPases to regulate stomatal apertures during pathogen attack. PLoS-Biology 7(6) 
  • Fuglsang AT, Guo Y, Cuin TA, Qiu Q, Song C, Kristiansen KA et al (2007) Arabidopsis protein kinase PKS5 inhibits the plasma membrane H+-ATPase by preventing interaction with 14-3-3 protein. Plant Cell 19(5):1617-1634 
  • Niittyla T, Fuglsang AT, Palmgren MG, Frommer WB, Schulze WX (2007). Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis. Molecular and Cellular Proteomics 6(10):1711-1726