Thomas Günther-Pomorski

Thomas Günther-Pomorski

Professor


  1. 2024
  2. Published

    Direct evidence of lipid transport by the Drs2–Cdc50 flippase upon truncation of its terminal regions

    Herrera, S. A., Justesen, B. H., Dieudonné, T., Montigny, C., Nissen, P., Lenoir, G. & Pomorski, Thomas Guenther, 2024, In: Protein Science. 33, 3, 12 p., e4855.

    Research output: Contribution to journalJournal articlepeer-review

  3. E-pub ahead of print

    Electroformation of giant unilamellar vesicles from large liposomes

    Uzun, Huriye Deniz, Tiris, Z., Czarnetzki, M., Lopez Marques, Rosa Laura & Pomorski, Thomas Guenther, 2024, (E-pub ahead of print) In: European Physical Journal: Special Topics.

    Research output: Contribution to journalJournal articlepeer-review

  4. Published

    Oxetane ring formation in taxol biosynthesis is catalyzed by a bifunctional cytochrome P450 enzyme

    Zhao, Yong, zpm303, zpm303, Xie, Yuman, Duan, Y. T., Andeadelli, A., Pateraki, Irini, Makris, A. M., Pomorski, Thomas Guenther, Stærk, Dan & Kampranis, Sotirios, 2024, In: Journal of the American Chemical Society. 146, p. 801-810

    Research output: Contribution to journalJournal articlepeer-review

  5. 2023
  6. Published

    Anionic Phospholipids Stimulate the Proton Pumping Activity of the Plant Plasma Membrane P-Type H+-ATPase

    Paweletz, L. C., Holtbrügge, S. L., Löb, M., De Vecchis, D., Schäfer, L. V., Pomorski, Thomas Guenther & Justesen, B. H., 2023, In: International Journal of Molecular Sciences. 24, 17, 21 p., 13106.

    Research output: Contribution to journalJournal articlepeer-review

  7. Published

    Determination of membrane protein orientation upon liposomal reconstitution down to the single vesicle level

    Veit, S., Paweletz, L. C. & Pomorski, Thomas Guenther, 2023, In: Biological Chemistry. 404, 7

    Research output: Contribution to journalReviewpeer-review

  8. Published

    Functional Analysis of the P-Type ATPases Apt2-4 from Cryptococcus neoformans by Heterologous Expression in Saccharomyces cerevisiae

    Veit, S., Laerbusch, S., Lopez Marques, Rosa Laura & Pomorski, Thomas Guenther, 2023, In: Journal of Fungi. 9, 2, 16 p., 202.

    Research output: Contribution to journalJournal articlepeer-review

  9. Published

    Measuring in vitro ATPase Activity with High Sensitivity Using Radiolabeled ATP

    Veit, S. & Pomorski, Thomas Guenther, 2023, In: Bio-protocol. 13, 10, 14 p., e4676.

    Research output: Contribution to journalJournal articlepeer-review

  10. Published

    Reconstitution of ATP-dependent lipid transporters: gaining insight into molecular characteristics, regulation, and mechanisms

    Herrera, S. A. & Pomorski, Thomas Guenther, 2023, In: Bioscience Reports. 43, 8, 17 p.

    Research output: Contribution to journalReviewpeer-review

  11. Published

    Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors

    Wiesner, W., Kühnel, R. M. & Pomorski, Thomas Guenther, 2023, In: Bio-protocol. 13, 11, e4694.

    Research output: Contribution to journalJournal articlepeer-review

  12. Published

    Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining

    Baum, Julia Franziska, Uzun, Huriye Deniz & Pomorski, Thomas Guenther, 2023, In: Bio-protocol. 13, 14, e4754.

    Research output: Contribution to journalJournal articlepeer-review

  13. Published

    Visualizing NBD-lipid Uptake in Mammalian Cells by Confocal Microscopy

    Baum, Julia Franziska, Bredegaard, L., Herrera, S. A. & Pomorski, Thomas Guenther, 2023, In: Bio-protocol. 13, 13, 16 p., e4771.

    Research output: Contribution to journalJournal articlepeer-review

  14. 2022
  15. Published

    A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles

    Mathiassen, P. P. M. & Pomorski, Thomas Guenther, 2022, In: Bio-protocol. 12, 6, 19 p., 4366.

    Research output: Contribution to journalJournal articlepeer-review

  16. Published

    Autoinhibition and regulation by phosphoinositides of ATP8B1, a human lipid flippase associated with intrahepatic cholestatic disorders

    Dieudonné, T., Herrera, S. A., Laursen, M. J., Lejeune, M., Stock, C., Slimani, K., Jaxel, C., Lyons, J. A., Montigny, C., Pomorski, Thomas Guenther, Nissen, P. & Lenoir, G., 2022, In: eLife. 11, 33 p., e75272.

    Research output: Contribution to journalJournal articlepeer-review

  17. Published

    CDC50A is required for aminophospholipid transport and cell fusion in mouse C2C12 myoblasts

    Grifell-Junyent, M., Baum, J. F., Valimets, S., Herrmann, A., Paulusma, C. C., Lopez Marques, Rosa Laura & Pomorski, Thomas Guenther, 2022, In: Journal of Cell Science. 135, 5, jcs258649.

    Research output: Contribution to journalJournal articlepeer-review

  18. Effects of 4-Br-A23187 on Bacillus subtilis cells and unilamellar vesicles reveal it to be a potent copper ionophore

    Senges, C. H. R., Warmuth, H. L., Vázquez‐Hernández, M., Uzun, Huriye Deniz, Sagurna, L., Dietze, P., Schmidt, C., Mücher, B., Herlitze, S., Krämer, U., Ott, I., Pomorski, Thomas Guenther & Bandow, J. E., 2022, In: Proteomics. 22, 17, 12 p., 2200061.

    Research output: Contribution to journalJournal articlepeer-review

  19. Published

    In vitro Assay to Evaluate Cation Transport of Ionophores

    Uzun, Huriye Deniz, Vázquez-Hernández, M., Bandow, J. & Pomorski, Thomas Guenther, 2022, In: Bio-protocol. 12, 22, 11 p., e4552.

    Research output: Contribution to journalJournal articlepeer-review

  20. Published

    Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation

    Johansen, Nicolai Tidemand, Bonaccorsi, M., Bengtsen, T., Nørgrd Larsen, Andreas, Tidemand, Frederik Grønbæk, Pedersen, M. C., Huda, P., Berndtsson, J., Darwish, T., Yepuri, N. R., Martel, A., Pomorski, Thomas Guenther, Bertarello, A., Sansom, M., Rapp, M., Crehuet, R., Schubeis, T., Lindorff-Larsen, Kresten, Pintacuda, G. & Arleth, Lise, 2022, In: eLife. 11, 35 p.

    Research output: Contribution to journalJournal articlepeer-review

  21. Published

    NBD-lipid Uptake Assay for Mammalian Cell Lines

    Herrera, S. A., Grifell-Junyent, M. & Pomorski, Thomas Guenther, 2022, In: Bio-protocol. 12, 4, 258649.

    Research output: Contribution to journalJournal articlepeer-review

  22. Published

    Single Vesicle Fluorescence-Bleaching Assay for Multi-Parameter Analysis of Proteoliposomes by Total Internal Reflection Fluorescence Microscopy

    Veit, S., Paweletz, L. C., Bohr, S. S., Menon, A. K., Hatzakis, Nikos & Pomorski, Thomas Guenther, 2022, In: ACS applied materials & interfaces. 14, 26, p. 29659−29667 9 p.

    Research output: Contribution to journalJournal articlepeer-review

  23. 2021
  24. Published

    Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter

    Mathiassen, P. P. M., Menon, A. K. & Pomorski, Thomas Guenther, 2021, In: Scientific Reports. 11, 1, 14 p., 14364.

    Research output: Contribution to journalJournal articlepeer-review

  25. Published

    Functional Significance of Conserved Cysteines in the Extracellular Loops of the ATP Binding Cassette Transporter Pdr11p

    Stanchev, L. D., Marek, M., Xian, F., Klöhn, M., Silvestro, D., Dittmar, G., Lopez Marques, Rosa Laura & Pomorski, Thomas Guenther, 2021, In: Journal of Fungi. 7, 1, 16 p., 2.

    Research output: Contribution to journalJournal articlepeer-review

  26. Published

    Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy

    Lopez Marques, Rosa Laura & Pomorski, Thomas Guenther, 2021, In: Bio-protocol. 11, 22, e4228.

    Research output: Contribution to journalJournal articlepeer-review

  27. Published

    P-type atpase apt1 of the fungal pathogen Cryptococcus neoformans is a lipid flippase of broad substrate specificity

    Stanchev, L. D., Rizzo, J., Peschel, R., Pazurek, L. A., Bredegaard, L., Veit, S., Laerbusch, S., Rodrigues, M. L., Lopez Marques, Rosa Laura & Pomorski, Thomas Guenther, 2021, In: Journal of Fungi. 7, 10, 16 p., 843.

    Research output: Contribution to journalJournal articlepeer-review

  28. Published

    Structural requirements for membrane binding of human guanylate-binding protein 1

    Sistemich, L., Dimitrov Stanchev, L., Kutsch, M., Roux, A., Pomorski, Thomas Guenther & Herrmann, C., 2021, In: FEBS Journal. 288, 13, p. 4098-4114

    Research output: Contribution to journalJournal articlepeer-review

  29. 2020
  30. Published

    Pseudohyphal growth in Saccharomyces cerevisiae involves protein kinase-regulated lipid flippases

    Frøsig, M. M., Costa, S. R., Liesche, J., Østerberg, J. T., Hanisch, S., Kjeldgaard-Nintemann, Sebastian J, Sørensen, Helle, Palmgren, Michael, Pomorski, Thomas Guenther & Lopez Marques, Rosa Laura, 6 Aug 2020, In: Journal of Cell Science. 133, 15, 235994.

    Research output: Contribution to journalJournal articlepeer-review

  31. Published

    Label-Free Fluorescence Quantification of Hydrolytic Enzyme Activity on Native Substrates Reveals How Lipase Function Depends on Membrane Curvature

    Bohr, S. S., Thorlaksen, C., Kühnel, R. M., Pomorski, Thomas Guenther & Hatzakis, Nikos, 16 Jun 2020, In: Langmuir. 36, 23, p. 6473-6481 9 p.

    Research output: Contribution to journalJournal articlepeer-review

  32. Agrobacterium tumefaciens Small Lipoprotein Atu8019 Is Involved in Selective Outer Membrane Vesicle (OMV) Docking to Bacterial Cells

    Knoke, L. R., Herrera, S. A., Götz, K., Justesen, B. H., Pomorski, Thomas Guenther, Fritz, C., Schäkermann, S., Bandow, J. E. & Aktas, M., 9 Jun 2020, In: Frontiers in Microbiology. 11, 1228.

    Research output: Contribution to journalJournal articlepeer-review

  33. Published

    Small-angle Neutron Scattering Shows that the Solution Structures of the Bacterial Mg2+-Channel CorA are Overall Similar with and without Mg2+ Bound

    Arleth, Lise, Johansen, Nicolai Tidemand, Bengtsen, T., Larsen, A. H., Tidemand, Frederik Grønbæk, Pomorski, Thomas Guenther & Lindorff-Larsen, Kresten, 7 Feb 2020, In: Biophysical Journal. 118, 3, Suppl. 1, p. 12A 1 p.

    Research output: Contribution to journalConference abstract in journal

  34. Improved quantum efficiency in an engineered light harvesting/photosystem II super-complex for high current density biophotoanodes

    Hartmann, V., Harris, D., Bobrowski, T., Ruff, A., Frank, A., Pomorski, Thomas Guenther, Rögner, M., Schuhmann, W., Adir, N. & Nowaczyk, M. M., 2020, In: Journal of Materials Chemistry A. 8, 29, p. 14463-14471 9 p.

    Research output: Contribution to journalJournal articlepeer-review

  35. Published

    Primary cell wall inspired micro containers as a step towards a synthetic plant cell

    Paulraj, T., Wennmalm, S., Wieland, D. C. F., Riazanova, A. V., Dėdinaitė, A., Pomorski, Thomas Guenther, Cárdenas, M. & Svagan, A. J., 2020, In: Nature Communications. 11, 1, 11 p., 958.

    Research output: Contribution to journalJournal articlepeer-review

  36. Published

    The transport mechanism of P4 ATPase lipid flippases

    Lopez Marques, Rosa Laura, Gourdon, Pontus Emanuel, Pomorski, Thomas Guenther & Palmgren, Michael, 2020, In: Biochemical Journal. 477, 19, p. 3769-3790 22 p.

    Research output: Contribution to journalReviewpeer-review

  37. 2019
  38. Published

    The P5A ATPase Spf1p is stimulated by phosphatidylinositol 4-phosphate and influences cellular sterol homeostasis

    Sørensen, D. M., Holen, H. W., Pedersen, J. T., Martens, Helle Jakobe, Silvestro, D., Stanchev, L. D., Costa, S. R., Pomorski, Thomas Guenther, Lopez Marques, Rosa Laura & Palmgren, Michael, 15 Apr 2019, In: Molecular Biology of the Cell. 30, 9, p. 1069-1084

    Research output: Contribution to journalJournal articlepeer-review

  39. Published

    Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake

    Liu, X., Li, J., Zhao, H., Liu, B., Pomorski, Thomas Guenther, Chen, S. & Liesche, J., Apr 2019, In: Journal of Cell Biology. 218, 4, p. 1407-1420

    Research output: Contribution to journalJournal articlepeer-review

  40. Published

    The lipid head group is the key element for substrate recognition by the P4 ATPase ALA2: A phosphatidylserine flippase

    Theorin, L., Faxén, K., Sørensen, D. M., Migotti, R., Dittmar, G., Schiller, J., Daleke, D. L., Palmgren, Michael, Lopez Marques, Rosa Laura & Pomorski, Thomas Guenther, 6 Mar 2019, In: Biochemical Journal. 476, 5, p. 783-794

    Research output: Contribution to journalJournal articlepeer-review

  41. Published

    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy

    Liu, X., Pomorski, Thomas Guenther & Liesche, J., 2019, In: Bio-protocol. 9, 16

    Research output: Contribution to journalJournal articlepeer-review

  42. Published

    Role of lipid transporters in fungal physiology and pathogenicity

    Rizzo, J., Stanchev, L. D., da Silva, V. K. A., Nimrichter, L., Pomorski, Thomas Guenther & Rodrigues, M. L., 2019, In: Computational and Structural Biotechnology Journal. 17, p. 1278-1289 12 p.

    Research output: Contribution to journalReviewpeer-review

  43. Published

    Short-chain lipid-conjugated pH sensors for imaging of transporter activities in reconstituted systems and living cells

    Kuehnel, R. M., Grifell-Junyent, M., Jørgensen, I. L., Kemmer, G. C., Schiller, J., Palmgren, Michael, Justesen, B. H. & Pomorski, Thomas Guenther, 2019, In: Analyst. 144, 9, p. 3030-3037

    Research output: Contribution to journalJournal articlepeer-review

  44. Published

    Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes

    Maric, S., Lind, T. K., Raida, M. R., Bengtsson, E., Fredrikson, G. N., Rogers, S., Moulin, M., Haertlein, M., Forsyth, V. T., Wenk, M. R., Pomorski, Thomas Guenther, Arnebrant, T., Lund, R. & Cárdenas, M., 2019, In: Scientific Reports. 9, p. 1-14 7591.

    Research output: Contribution to journalJournal articlepeer-review

  45. 2018
  46. Published
  47. Published

    Ergosterol is mainly located in the cytoplasmic leaflet of the yeast plasma membrane

    Solanko, L. M., Sullivan, D. P., Sere, Y. Y., Szomek, M., Lunding, A., Solanko, K., Pizovic, A., Stanchev, L. D., Pomorski, Thomas Guenther, Menon, A. K. & Wüstner, D., 2018, In: Traffic. 19, 3, p. 198-214 7 p.

    Research output: Contribution to journalJournal articlepeer-review

  48. Published

    LEGO-inspired drug design: unveiling a class of Benzo[d]thiazoles containing a 3,4-Dihydroxyphenyl moiety as plasma membrane H+-ATPase inhibitors

    Thanh Tung, T., Dao, T. T., Grifell Junyent, M., Palmgren, Michael, Pomorski, Thomas Guenther, Fuglsang, Anja Thoe, Christensen, Søren Brøgger & Nielsen, J., 2018, In: ChemMedChem. 13, 1, 11 p.

    Research output: Contribution to journalJournal articlepeer-review

  49. 2017
  50. Published

    Membrane protein reconstitution into giant unilamellar vesicles: a review on current techniques

    Jørgensen, I. L., Kemmer, G. C. & Pomorski, Thomas Guenther, 2017, In: European Biophysics Journal. 46, 2, p. 103-119 17 p.

    Research output: Contribution to journalJournal articlepeer-review

  51. Published

    Phospholipid flipping involves a central cavity in P4 ATPases

    Jensen, M. S., Costa, S., Duelli, A. S., Andersen, P. A., Poulsen, L. R., Stanchev, L. D., Gourdon, Pontus Emanuel, Palmgren, Michael, Pomorski, Thomas Guenther & Lopez Marques, Rosa Laura, 2017, In: Scientific Reports. 7, 13 p., 17621.

    Research output: Contribution to journalJournal articlepeer-review

  52. Published

    Purification and characterisation of the yeast plasma membrane ATP binding cassette transporter Pdr11p

    Laub, K. R., Marek, M., Stanchev, L. D., Herrera, S. A., Kanashova, T., Bourmaud, A., Dittmar, G. & Pomorski, Thomas Guenther, 2017, In: PLOS ONE. 12, 9, 18 p., e0184236.

    Research output: Contribution to journalJournal articlepeer-review

  53. 2016
  54. Published

    Application of image cytometry to characterize heterologous lipid flippases in yeast

    Jensen, M. S., Costa, S., Theorin, L., Christensen, Jan Pravsgaard, Pomorski, Thomas Guenther & Lopez Marques, Rosa Laura, 2016, In: Cytometry. Part A. 89, 7, p. 673–680 8 p.

    Research output: Contribution to journalJournal articlepeer-review

  55. Published

    Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives

    Marek, M. & Pomorski, Thomas Guenther, 2016, P-Type ATPases: methods and protocols. Bublitz, M. (ed.). Springer, Vol. 2.. p. 181-191 11 p. (Methods in Molecular Biology, Vol. 1377).

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

  56. Published

    Biophysical study of resin acid effects on phospholipid membrane structure and properties

    Jagalski, V., Barker, R., Topgaard, D., Pomorski, Thomas Guenther, Hamberger, B. R. & Cardenas Gomez, M., 2016, In: BBA Biomembranes. 1858, 11, p. 2827-2838 12 p.

    Research output: Contribution to journalJournal articlepeer-review

  57. Published

    Cell-based lipid flippase assay employing fluorescent lipid derivatives

    Jensen, M. S., Costa, S., Pomorski, Thomas Guenther & Lopez Marques, Rosa Laura, 2016, P-Type ATPases: methods and protocols. Bublitz, M. (ed.). Springer, Vol. VI. p. 371-382 12 p. (Methods in Molecular Biology, Vol. 1377).

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

  58. Published

    Direct observation of proton pumping by a eukaryotic P-type ATPase

    Veshaguri, S., Christensen, S. F., Kemmer, G. C., Ghale, G., Møller, M. P., Lohr, C., Christensen, A. L., Justesen, B. H., Jørgensen, I. L., Schiller, J., Hatzakis, Nikos, Grabe, M., Pomorski, Thomas Guenther & Stamou, Dimitrios, 2016, In: Science. 351, 6280, p. 1469-1473 5 p.

    Research output: Contribution to journalJournal articlepeer-review

  59. Published

    Lipid somersaults: uncovering the mechanisms of protein-mediated lipid flipping

    Pomorski, Thomas Guenther & Menon, A. K., 2016, In: Progress in Lipid Research. 64, p. 69-84 16 p.

    Research output: Contribution to journalJournal articlepeer-review

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ID: 9099036